Abstract:
:Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and vig4-2 had amino acid substitutions in the C-terminal region, Ala286Val and Ser278Cys, respectively. In accord with these mutations, trimming of the C-terminal hydrophobic part close to the region impaired the function and traffic of the proteins from the endoplasmic reticulum to the Golgi compartments.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Abe M,Hashimoto H,Yoda Kdoi
10.1016/s0014-5793(99)01177-1keywords:
subject
Has Abstractpub_date
1999-09-24 00:00:00pages
309-12issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01177-1journal_volume
458pub_type
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