Abstract:
:Hemoglobin-binding proteins are necessary for pathogens to obtain iron from Hb. Entamoeba histolytica can grow using Hb as source of iron, but the underlying mechanism has not previously been established. In this work, we identified a 45 kDa Hb-binding protein of E. histolytica, which we named Ehhmbp45. In silico analysis showed that Ehhmbp45 contains the conserved domains needed for Hb-binding, while overlay assays demonstrated that Ehhmbp45 is able to bind Hb. In addition, we found that Ehhmbp45 mRNA levels were up-regulated under iron starvation conditions and were subsequently restored to basal levels when Hb was added to the cell cultures. These findings provide the first insights on the role of Ehhmbp45 in iron acquisition from Hb.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Cruz-Castañeda A,Olivares-Trejo JJdoi
10.1016/j.febslet.2008.06.057subject
Has Abstractpub_date
2008-08-06 00:00:00pages
2806-10issue
18eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00592-9journal_volume
582pub_type
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