Abstract:
:In addition to the selective amplification of cDNA from total RNA by the PCR method, the distinctive properties of ferredoxin-expressing colonies can be used for cloning a ferredoxin cDNA. This strategy for cloning and expressing cDNA in E. coli was applied to a sheep adreno-ferredoxin. The expressed sheep ferredoxin showed a spectral pattern typical of [2Fe-2S] proteins. The amino acid sequence deduced from the DNA sequence showed that the mature form of sheep ferredoxin consists of 128 amino acid residues. This rapid and simple method for cloning and expressing cDNA can be applied to other ferredoxins.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Matsuo Y,Tsujita M,Mizoguchi K,Ichikawa Ydoi
10.1016/0014-5793(92)81232-bkeywords:
subject
Has Abstractpub_date
1992-04-20 00:00:00pages
132-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(92)81232-Bjournal_volume
301pub_type
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