Free sphingobases induce RBOHD-dependent reactive oxygen species production in Arabidopsis leaves.

abstract：:Secreted proteins are indispensable for the development and differentiation of multicellular organisms. Cloning and characterization of novel or hypothetical genes encoding these proteins are therefore inviting great incentives. Using bioinformatics tools and experimental approaches, we isolated and characterized a hu...

journal_title：FEBS letters

authors： Zeng LC,Liu F,Zhang X,Zhu ZD,Wang ZQ,Han ZG,Ma WJ

更新日期：2004-07-30 00:00:00

abstract：:The relationship between ground state cis-trans isomerization and protonation state is explored for a model green fluorescent protein chromophore, 4-hydroxybenzylidene-1,2-dimethylimidazolinone (HBDI). We find that the protonation state has only a modest effect on the free energy differences between cis and trans isom...

journal_title：FEBS letters

authors： He X,Bell AF,Tonge PJ

更新日期：2003-08-14 00:00:00

abstract：:Measurements of the effects of NH3/NH4+ on glucose-induced electrical activity in beta-cells from microdissected mouse islets of Langerhans and on intracellular pH in single collagenase-isolated islets pre-loaded with a fluorescent pH probe were performed and are reported here. Application of NH3/NH4+ (15 mM) in the p...

journal_title：FEBS letters

authors： Rosario LM,Rojas E

更新日期：1986-05-05 00:00:00

abstract：:Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M, interferon-gamma 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or i...

journal_title：FEBS letters

authors： Harrison M,Dunger AM,Berg S,Mabley J,John N,Green MH,Green IC

更新日期：1998-09-18 00:00:00

abstract：:The primary structure of rhodopsin from the octopus Paroctopus defleini has been determined by parallel analysis of the protein and corresponding cDNA. The amino acid sequence is most similar to the recently cloned Drosophila opsins. Similarities to bovine and human opsins are also evident. The transmembrane topology ...

journal_title：FEBS letters

authors： Ovchinnikov YuA,Abdulaev NG,Zolotarev AS,Artamonov ID,Bespalov IA,Dergachev AE,Tsuda M

更新日期：1988-05-09 00:00:00

abstract：:Incubation of phosphatidylethanolamine containing liposomes with malondialdehyde and other aldehydes with different chain lengths results in the fluorescence of chromolipids. Relative to malondialdehyde, the fluorescence was greatly enhanced with increasing chain length upon incubation of 2-alkenals with phospholipids...

journal_title：FEBS letters

authors： Montfoort A,Bezstarosti K,Groh MM,Koster JF

更新日期：1987-12-21 00:00:00

abstract：:The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expr...

journal_title：FEBS letters

authors： Bae SH,Kim DH,Shin SK,Choi JS,Park KS

更新日期：2014-01-03 00:00:00

abstract：:The full-length cDNA encoding the human calbindin-D9k (CaBP-9k) has been cloned using reverse transcription/PCR methodology with rat- and bovine-derived primers and intestinal RNA. A core product, and both a 5' and 3' product encompassing the full-length cDNA were obtained. The clones include coding region for 79 amin...

journal_title：FEBS letters

authors： Jeung EB,Krisinger J,Dann JL,Leung PC

更新日期：1992-07-28 00:00:00

abstract：:Cripto-1 and OCT4, expressed in stem cells and cancers, play important roles in tumorigenesis. Here, we demonstrate that Cripto-1 expression is regulated by OCT4 in human embryonic carcinoma NCCIT cells. The endogenous expression of Cripto-1 and OCT4 is significantly reduced during differentiation. Cripto-1 expression...

journal_title：FEBS letters

authors： Park SW,Do HJ,Han MH,Choi W,Kim JH

更新日期：2018-01-01 00:00:00

abstract：:The macromolecular diversity and concentrations in the fluid phase of cytoplasm constitute conditions necessary and sufficient for aqueous phase separation. Consequences of phase separation in cytoplasm, including its 'compartmentation', are inferred from analogies with the physicochemical properties of aqueous two-ph...

journal_title：FEBS letters

authors： Walter H,Brooks DE

更新日期：1995-03-20 00:00:00

abstract：:Tyrosine-rich acidic matrix protein (TRAMP; 22 kDa extracellular matrix protein; dermatopontin) is a protein that co-purifies with lysyl oxidase and with dermatan sulphate proteoglycans, with possible functions in cell-matrix interactions and matrix assembly. Using a rabbit polyclonal antiserum raised against porcine ...

journal_title：FEBS letters

authors： Forbes EG,Cronshaw AD,MacBeath JR,Hulmes DJ

更新日期：1994-09-12 00:00:00

abstract：:There are two secondary structure models for the eukaryotic selenocysteine (Sec) tRNA(Sec). One model, the 9/4 structure, was experimentally tested and possesses acceptor and T-stems with 9 and 4 bp, respectively [Sturchler et al., 1993; Hubert et al., 1998]. The other one, the 7/5 secondary structure with a bulge in ...

journal_title：FEBS letters

authors： Mizutani T,Goto C

更新日期：2000-01-28 00:00:00

abstract：:Specific binding sites for 3,3'-T2 can be detected in swollen and osmotically treated mitochondria (OTM) from normal and hypothyroid rat liver. In hypothyroid animals, maximal values of binding were obtained at 0 degrees C while values were lower at 37 degrees C and no specific binding could be observed at 60 degrees ...

journal_title：FEBS letters

authors： Lanni A,Moreno M,Horst C,Lombardi A,Goglia F

更新日期：1994-09-05 00:00:00

abstract：:Amino acid sequencing of a 48/46 kDa glycoprotein from human plantar callus, recognised by antisera raised against the desmosomal cadherins DGII/III, has revealed N-terminal homology to the DNA-derived sequence of human and bovine DGII/III. However, a tryptic fragment has homology only with a bovine clone. We propose ...

journal_title：FEBS letters

authors： King IA,Magee AI,Rees DA,Buxton RS

更新日期：1991-07-29 00:00:00

abstract：:Post-absorptive gluconeogenesis from lactate measured in vivo increases 3-fold in 24-month-old rats compared to 3-month-old animals. Fractional lactate turnover rates showed no significant differences between the two groups of animals. Lower plasma glucose concentrations and insulin-glucagon ratios may explain the inc...

journal_title：FEBS letters

authors： Satrústegui J,Cuezva JM,Machado A

更新日期：1986-03-03 00:00:00

abstract：:The rise of antibiotic resistance combined with the lack of new products entering the market has led to bacterial infections becoming one of the biggest threats to global health. Therefore, there is an urgent need to identify novel antibiotic targets, such as dihydrodipicolinate synthase (DHDPS), an enzyme involved in...

journal_title：FEBS letters

authors： Impey RE,Lee M,Hawkins DA,Sutton JM,Panjikar S,Perugini MA,Soares da Costa TP

更新日期：2020-05-01 00:00:00

abstract：:c-Myc is a transcriptional regulator involved in carcinogenesis through its role in growth control and cell cycle progression. Here we attempt to relate its role in stimulating the G1-S transition to the ability to affect functioning of key cell cycle regulators, and we focus on how its property of modulating transcri...

journal_title：FEBS letters

authors： Nasi S,Ciarapica R,Jucker R,Rosati J,Soucek L

更新日期：2001-02-16 00:00:00

abstract：:We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica, aerotolerant anaerobic Bacteroides fragilis, and facultative anaerobic Salmonella typhimurium after exposure to O2 or H2O2. Using HPLC with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8OHdG) as a damage marker. O2 ind...

journal_title：FEBS letters

authors： Takeuchi T,Nakaya Y,Kato N,Watanabe K,Morimoto K

更新日期：1999-05-07 00:00:00

abstract：:Sialic acids comprise a family of terminal sugars essential for a variety of biological recognition systems. UDP-N-acetylglucosamine 2-epimerase catalyzes the first step of their biosynthesis. Periodate-oxidized UDP-N-acetylglucosamine, namely 2',3'-dialdehydo-UDP-alpha-D-N-acetylglucosamine, was found to be an effect...

journal_title：FEBS letters

authors： Blume A,Chen H,Reutter W,Schmidt RR,Hinderlich S

更新日期：2002-06-19 00:00:00

abstract：:The ubiquitin-like protein Urm1 from budding yeast and its E1-like activator Uba4 have dual roles in protein urmylation and tRNA thiolation pathways. To study whether these are conserved among eukaryotes, we used gene shuffles to replace the yeast proteins by their human counterparts, hURM1 and hUBA4/MOCS3. As judged ...

journal_title：FEBS letters

authors： Jüdes A,Ebert F,Bär C,Thüring KL,Harrer A,Klassen R,Helm M,Stark MJ,Schaffrath R

更新日期：2015-04-02 00:00:00

abstract：:A simple model is put forward to explain the long-known three-base periodicity in coding DNA. We propose the concept of same-phase triplet clustering, i.e. a condition wherein a triplet appears several times in one phase without interruption by the two other possible phases. For instance, in the sequence (i): NTT_GNN_...

journal_title：FEBS letters

authors： Sánchez J,López-Villaseñor I

更新日期：2006-11-27 00:00:00

abstract：:Oil crops are in increasing demand both for food and as renewable sources of chemicals. It is therefore vital to understand how oil accumulation is regulated. Different ways of obtaining such information are discussed with an emphasis on metabolic control analysis. The usefulness of the latter has been well-illustrate...

journal_title：FEBS letters

authors： Harwood JL,Guschina IA

更新日期：2013-06-27 00:00:00

abstract：:The adipose-derived plasma protein, adiponectin (APN), has various protective effects on cardiovascular diseases. In this study, we show that endogenous APN is required for full cyclooxygenase-2 (COX-2) induction by ischemia-reperfusion injury in the heart in vivo. In rat neonatal cardiac myocytes, APN-induced COX-2 e...

journal_title：FEBS letters

authors： Ikeda Y,Ohashi K,Shibata R,Pimentel DR,Kihara S,Ouchi N,Walsh K

更新日期：2008-04-02 00:00:00

abstract：:The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...

journal_title：FEBS letters

authors： Miyamoto K,Nishimura K,Masuda T,Tsuji H,Inokuchi H

更新日期：1992-10-05 00:00:00

abstract：:The angiotensin II type I (AT(1)) receptor mediates regulation of blood pressure and water-electrolyte balance by Ang II. Substitution of Gly for Asn(111) of the AT(1) receptor constitutively activates the receptor leading to Gq-coupled IP(3) production independent of Ang II binding. The Ang II-activated conformation ...

journal_title：FEBS letters

authors： Lee C,Hwang SA,Jang SH,Chung HS,Bhat MB,Karnik SS

更新日期：2007-05-29 00:00:00

abstract：:The present report provides evidence that, in A431 cells, interferon gamma (IFNgamma) induces the rapid (within 5 min), and reversible, tyrosine phosphorylation of the epidermal growth factor receptor (EGFR). IFNgamma-induced EGFR transactivation requires EGFR kinase activity, as well as activity of the Src-family tyr...

journal_title：FEBS letters

authors： Burova E,Vassilenko K,Dorosh V,Gonchar I,Nikolsky N

更新日期：2007-04-03 00:00:00

abstract：:Inducible heat shock protein 70 (Hsp70) is one of the most important HSPs for maintenance of cell integrity during normal cellular growth as well as pathophysiological conditions. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial signaling transducer that regulates a diverse array of physiologica...

journal_title：FEBS letters

authors： Chen H,Wu Y,Zhang Y,Jin L,Luo L,Xue B,Lu C,Zhang X,Yin Z

更新日期：2006-05-29 00:00:00

abstract：:A series of oligomeric double and triple helical DNAs with irregular sequences of homopurine and homopyrimidine strands were prepared. DNA triplexes were identified by CD spectroscopy and thermal denaturation profiles (biphasic helix-coil transition). Condensation of oligonucleotides on single and double-stranded DNA ...

journal_title：FEBS letters

authors： Dolinnaya NG,Pyatrauskene OV,Shabarova ZA

更新日期：1991-06-24 00:00:00

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:Two-dimensional hydrophobic clusters analysis (HCA) was used to compare the distribution of hydrophobic clusters along various actin sequence. HCA-deduced patterns were not altered by amino-acid variations throughout the evolution of actin and we observed similar hydrophobic motifs comprising myosin subfragment-1 ATP-...

journal_title：FEBS letters

authors： Labbé JP,Boyer M,Benyamin Y

更新日期：1995-10-16 00:00:00