Abstract:
:The angiotensin II type I (AT(1)) receptor mediates regulation of blood pressure and water-electrolyte balance by Ang II. Substitution of Gly for Asn(111) of the AT(1) receptor constitutively activates the receptor leading to Gq-coupled IP(3) production independent of Ang II binding. The Ang II-activated conformation of the AT1(N111G) receptor was proposed to be similar to that of the wild-type AT(1) receptor, although, various aspects of the Ang II-induced conformation of this constitutively active mutant receptor have not been systematically studied. Here, we provide evidence that the conformation of the active state of the wild-type and the constitutively active AT(1) receptors are different. Upon Ang II binding an activated conformation of the wild-type AT(1) receptor activates G protein and recruits beta-arrestin. In contrast, the agonist-bound AT1(N111G) mutant receptor preferentially couples to Gq and is inadequate in beta-arrestin recruitment.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lee C,Hwang SA,Jang SH,Chung HS,Bhat MB,Karnik SSdoi
10.1016/j.febslet.2007.04.069subject
Has Abstractpub_date
2007-05-29 00:00:00pages
2517-22issue
13eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00468-1journal_volume
581pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Combining mutation and chemical modification, we have introduced Asp, Gln, Cys, S-carboxymethylcysteine (Cax) and S-carbamoylmethylcysteine (Cam) into the positions of Glu190 and Glu201 of the beta subunit of F1-ATPase from the thermophilic Bacillus PS3. The steady-state ATPase activities of alpha 3 beta 3 gamma compl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00588-5
更新日期:1994-07-04 00:00:00
abstract::In endocrine cells the regulated secretion of hormones, peptides, enzymes and neurotransmitters into the external medium occurs when mature secretory granules fuse with the plasma membrane. Secretory granules form at the trans-Golgi network (TGN) by envelopment of the dense-core aggregate of regulated secretory protei...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(91)80805-d
更新日期:1991-07-22 00:00:00
abstract::The present study discusses the efficacy of Aloe emodin-8-O-glycoside (AEG), a plant derived anthroquinone, on alleviating insulin resistance and augmenting glycogen synthesis in L6 myotubes and 3T3L1 adipocytes. Dose-dependent increase in glucose uptake activity (GUA) was observed in both cell lines. Immunoblot analy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.004
更新日期:2010-07-16 00:00:00
abstract::The informational content of the primary structure of thymopoietin (TP) is investigated using the informational spectrum method (ISM). We show that the sequence of TP shares common information with the sequences of long postsynaptic snake neurotoxins, although no apparent similarity was found among their primary struc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80561-1
更新日期:1988-10-24 00:00:00
abstract::The amino acid sequence of the alpha-subunit of Gi, the human adenylate cyclase inhibiting GTP-binding protein, has been deduced from the nucleotide sequence of a DNA clone complementary to Gi alpha mRNA from differentiated U937 cells. The cDNA encodes a polypeptide of 355 amino acids (Mr 40456). The amino acid sequen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81428-x
更新日期:1987-01-26 00:00:00
abstract::Yeast tRNA(Lys)CUU is nucleus-encoded and is partially imported into the mitochondria. Another lysine isoacceptor, tRNA(Lys)SUU, is also nucleus-encoded but is not imported. These two tRNAs differ in 21 bases. We have previously localised import selectivity determinants in the anticodon arm. By in vitro import of muta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01653-6
更新日期:1999-01-15 00:00:00
abstract::Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80876-k
更新日期:1990-06-04 00:00:00
abstract::Measurement of the weight of desmosterol produced during its biosynthesis in the presence of tritiated water and triparanol has permitted a direct determination of the relative flux of carbon and tritium (the H/C ratio) into sterol in hepatocytes. The H/C ratio increased with time of incubation irrespective of the nut...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81264-3
更新日期:1985-08-05 00:00:00
abstract::6-Phosphofructo-2-kinase catalyzes the synthesis and degradation of fructose 2,6-bisphosphate, activator of phosphofructokinase-1 and inhibitor of fructose 1,6-bisphosphatase. These properties confer to this bifunctional enzyme a key role in the control of glycolysis and gluconeogenesis. Several mammalian isozymes gen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00808-1
更新日期:2003-08-28 00:00:00
abstract::Vascular endothelial growth factor (VEGF) mRNA expression was analysed in rabbit vascular smooth muscle cells following exposure to hypoxia and platelet-derived growth factor-BB (PDGF-BB). Hypoxia potently upregulated VEGF mRNA steady-state levels in a time- and concentration-dependent manner reaching a maximum level ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01458-d
更新日期:1995-01-30 00:00:00
abstract::Interactions of U2 snRNA with anti-Sm precipitable proteins in RNA-free cytoplasmic complexes were analyzed. U2 snRNA was found to bind specifically with proteins in the 6S complexes but not in the 20S complexes. The binding activity was preserved using U2 snRNA having a mutated Sm binding site. Label-transfer experim...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00095-1
更新日期:1997-03-03 00:00:00
abstract::As part of a search for selective, mechanism-based covalent inhibitors of human pancreatic α-amylase we describe the chemoenzymatic synthesis of the disaccharide analog α-glucosyl epi-cyclophellitol, demonstrate its stoichiometric reaction with human pancreatic α-amylase and evaluate the time dependence of its inhibit...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12143
更新日期:2016-04-01 00:00:00
abstract::Escherichia coli maltose-binding protein (MBP) is frequently used as an affinity tag to facilitate the purification of recombinant proteins. An important additional attribute of MBP is its remarkable ability to enhance the solubility of its fusion partners. MBPs are present in a wide variety of microorganisms includin...
journal_title:FEBS letters
pub_type: 历史文章,杂志文章
doi:10.1016/s0014-5793(03)00070-x
更新日期:2003-02-27 00:00:00
abstract::A steady-state framework was applied to the ubiquitous tricyclic enzyme cascade structure, as seen in the mitogen-activated protein (MAP) kinase system, to analyze the effect of upstream kinase concentrations on final output response. The results suggest that signal amplification achieved by the cascade structure ensu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00021-3
更新日期:2004-01-30 00:00:00
abstract::To identify the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFcgamma2R), peptides derived from the membrane-distal extracellular domain (EC1) of boFcgamma2R corresponding to the homologous region of human FcalphaRI were synthesized. Binding of bovine IgG2 to the different peptides was tested by Dot-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.060
更新日期:2006-02-20 00:00:00
abstract::NELL1 is an extracellular protein inducing osteogenic differentiation and bone formation of osteoblastic cells. To elucidate the intracellular signaling cascade evoked by NELL1, we have shown that NELL1 protein transiently activates the MAPK signaling cascade, induces the phosphorylation of Runx2, and promotes the rap...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.12.006
更新日期:2008-01-23 00:00:00
abstract::Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.10.061
更新日期:2004-12-03 00:00:00
abstract::The concentration of insulin that produces half-maximal stimulation of glycolysis by stripped soleus muscle preparations is markedly increased by the adenosine analogues, 2-chloroadenosine and N6-phenylisopropyladenosine, but is markedly decreased by the methyl xanthine analogue, 8-phenyltheophylline. 2-Chloroadenosin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80820-0
更新日期:1984-02-13 00:00:00
abstract::This study describes the comparative analysis of two insect recombinant aminotransferases, Aedes aegypti 3-hydroxykynurenine (3-HK) transaminase/alanine glyoxylate aminotransferase (Ae-HKT/AGT) and Drosophila melanogaster serine pyruvate aminotransferase (Dm-Spat), which share 52% identity in their amino acid sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03229-5
更新日期:2002-09-11 00:00:00
abstract::Conformational changes in the extradiscal regions of rhodopsin induced by illumination were investigated by modifying the visual pigment by mild treatment with cyanogen bromide prior to and after light exposure. Light induced an increased yield of cleavage of the Met bond 253-254 and a new cleavage at the Met bond 155...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81138-8
更新日期:1985-02-11 00:00:00
abstract::Immunometabolism focuses on the mechanisms regulating the impact of metabolism on lymphocyte activity and autoimmunity outbreak. The adipose tissue is long known to release adipokines, either pro- or anti-inflammatory factors bridging nutrition and immune function. More recently, adipocytes were discovered to also rel...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12733
更新日期:2017-10-01 00:00:00
abstract::In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddyla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.04.011
更新日期:2012-05-21 00:00:00
abstract::DNA polymerase (pol) λ, one of the 15 cellular pols, belongs to the X family. It is a small 575 amino-acid protein containing a polymerase, a dRP-lyase, a proline/serine rich and a BRCT domain. Pol λ shows various enzymatic activities including DNA polymerization, terminal transferase and dRP-lyase. It has been implic...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.03.069
更新日期:2011-09-16 00:00:00
abstract::NADP-malic enzyme (NADP-ME) is a widely distributed enzyme that catalyzes the oxidative decarboxylation of L-malate. Photosynthetic NADP-MEs are found in C4 bundle sheath chloroplasts and in the cytosol of CAM plants, while non-photosynthetic NADP-MEs are either plastidic or cytosolic in various plants. We propose a c...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(00)02331-0
更新日期:2001-02-09 00:00:00
abstract::A tropomyosin-binding protein (app. Mr 17000) was detected in equine platelet preparations by a gel overlay technique. Its isolation, amino acid and partial sequence analyses have shown it to be histone H2B. As with a similar protein from pig platelet preparations [der Terrossian et al. (1983) FEBS Lett. 152, 202-206]...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81130-2
更新日期:1983-06-27 00:00:00
abstract::The amino acid sequence of the zinc-requiring beta-lactamase II from Bacillus cereus strain 569 has been determined. It consists of a single polypeptide chain of 227 residues. It is the only example so far fully characterized of a class B beta-lactamase, and is structurally and mechanistically distinct from both the w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81024-3
更新日期:1985-09-23 00:00:00
abstract::Following the biophysical analysis of plant K+ channels in their natural environment, three members from the green branch of the evolutionary tree of life KAT1, AKT1, and KST1 have recently been identified on the molecular level. Among them, we focussed on the expression and characterization of the Arabidopsis thalian...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00042-7
更新日期:1996-02-19 00:00:00
abstract::The influence of aniso-osmolarity on the activity of the MAP kinases Erk-1 and Erk-2 was studied in C6 glioma cells. Hypo-osmotic treatment (205 mosmol/l) led to an increased activity of Erk-1 and Erk-2 within 3 min, which became maximal at 10 min and returned to basal level within 120 min. In contrast, Erk activity w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01376-2
更新日期:1997-01-03 00:00:00
abstract::Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01028-9
更新日期:1996-10-21 00:00:00
abstract::A specific b subunit arginine, b(Arg-36) in Escherichia coli, displays evolutionary conservation among bacterial F1F0 ATP synthases. Site-directed mutagenesis was used to generate a collection of mutations affecting b(Arg-36). The phenotype differed depending upon the substitution, and the b(Arg-36-Glu) and b(Arg-36-I...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00597-3
更新日期:1998-06-12 00:00:00