Abstract:
:A steady-state framework was applied to the ubiquitous tricyclic enzyme cascade structure, as seen in the mitogen-activated protein (MAP) kinase system, to analyze the effect of upstream kinase concentrations on final output response. The results suggest that signal amplification achieved by the cascade structure ensured that the modifying enzymes at various steps of the cascade were nearly saturated. Thus, there was no change in the response sensitivity with increasing upstream kinase concentration. Analysis was also extended to branching of a signaling pathway as an example of cross-talk. It was observed that the cascade structure confers a larger share of the signal transduction properties to its last kinase. This phenomenon in enzyme cascades may explain how the response of the terminal MAP kinase is unaffected by cross-talk of upstream kinases.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mutalik VK,Singh AP,Edwards JS,Venkatesh KVdoi
10.1016/S0014-5793(04)00021-3keywords:
subject
Has Abstractpub_date
2004-01-30 00:00:00pages
79-84issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304000213journal_volume
558pub_type
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