Abstract:
:Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the narrow resonance signal characterizing phospholipids inserted into a mixed micelle. Up to 0.25 g detergent/g protein could be incorporated into the membrane without solubilization. Higher detergent concentrations of up to 1.5-2 g detergent/g protein led to gradual solubilization. Although the method allows us to monitor the extent of solubilization of individual phospholipid classes, there was no evidence of either preferential solubilization or retention of a specific class of phospholipids.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Roux M,Champeil Pdoi
10.1016/0014-5793(84)80481-0subject
Has Abstractpub_date
1984-06-11 00:00:00pages
169-72issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(84)80481-0journal_volume
171pub_type
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