Abstract:
:alphaArg-376, betaLys-155, and betaArg-182 are catalytically important ATP synthase residues that were proposed to be involved in substrate Pi binding and subsequent steps of ATP synthesis [Senior, A.E., Nadanaciva, S. and Weber, J. (2002) Biochim. Biophys. Acta 1553, 188-211]. Here, it was shown using purified Escherichia coli F(1)-ATPase that whereas Pi protected wild-type from reaction with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, mutations betaK155Q, betaR182Q, betaR182K, and alphaR376Q abolished protection. Therefore, in ATP synthesis initial binding of substrate Pi in open catalytic site betaE is supported by each of these three residues.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ahmad Z,Senior AEdoi
10.1016/j.febslet.2004.12.022keywords:
subject
Has Abstractpub_date
2005-01-17 00:00:00pages
523-8issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(04)01557-1journal_volume
579pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The domains of the pore-forming protein responsible for the gate and channel formations were separated and identified in the outer membrane of Pseudomonas aeruginosa. The proteolytic cleavage of the 46K channel protein, protein D2, yielded two major domains with apparent M(r) of 27K and 19K. We identified the 27K poly...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80826-3
更新日期:1992-07-13 00:00:00
abstract::Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposur...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.007
更新日期:2006-12-11 00:00:00
abstract::Differentiation enhancing factor (DEF) is a 29 kDa protein expressed in murine erythroleukemia (MEL) cells and active in promoting a significant increase in the rate of hexamethylenebisacetamide induced differentiation of these cells. The factor was recently shown to possess an amino acid sequence identical to that re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00716-m
更新日期:1995-07-24 00:00:00
abstract::Farnesoic acid is a signaling molecule that inhibits the transition from budding yeast to filament formation in Candida albicans, but the molecular mechanism regulated by this substance is unknown. In this study, we analyzed the function of CaPHO81, which is induced by farnesoic acid. The pho81Δ mutant cells existed e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.10.026
更新日期:2010-11-19 00:00:00
abstract::The relationship between the antioxidant effects of reduced coenzyme Q10 (ubiquinol, UQH2) and vitamin E (alpha-tocopherol) was investigated in beef heart submitochondrial particles in which lipid peroxidation was initiated by incubation with ascorbate + ADP-Fe3+. These effects were examined after extraction of coenzy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80720-n
更新日期:1991-07-08 00:00:00
abstract::The rate of whole cell H2O2 metabolism in several salivarian and stercorarian trypanosomes and Leishmania species was measured. These cells metabolized H2O2 at rates between 2.3 and 48.2 nmol/10(8) cells per min depending upon the species employed. H2O2 metabolism was largely insensitive to NaN3, implying that typical...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80968-7
更新日期:1987-09-14 00:00:00
abstract::Exposure of Ehrlich ascites tumor cells to 5-azacytidine for 5 h resulted in a partial loss of ability of DNA to stimulate ADP-ribosyltransferase activity, as assessed in a reconstituted in vitro enzyme system consisting of purified calf thymus enzyme, calf thymus whole histone and DNA isolated from the cells. The deg...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80154-b
更新日期:1990-03-12 00:00:00
abstract::The glycine-alanine (GA) repeat of the Epstein-Barr virus nuclear antigen-1 inhibits in cis ubiquitin-dependent proteolysis in mammalian cells through a yet unknown mechanism. In the present study we demonstrate that the GA repeat targets an evolutionarily conserved step in proteolysis since it can prevent the degrada...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01296-1
更新日期:2003-12-04 00:00:00
abstract::The ubiquitin-like modifier, FAT10, is involved in proteasomal degradation and antigen processing. As ubiquitin and the ubiquitin-like modifier, ISG15, cotranslationally modify proteins, we investigated whether FAT10 could also be conjugated to newly synthesized proteins. Indeed, we found that nascent proteins are mod...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12512
更新日期:2017-01-01 00:00:00
abstract::To understand the role of nitric oxide (NO) in controlling the specific DNA-binding activities of transcriptional factors, we investigated the in vitro effect of the NO-donor sodium nitroprusside (SNP) on the AP-1 activity of cultured mouse cerebellar granule cells. A gel-mobility assay showed that SNP inhibited AP-1 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00839-6
更新日期:1994-08-29 00:00:00
abstract::Decades of neuroscience research have shed light on the hippocampus as a key structure for the formation of episodic memory. The hippocampus is divided into distinct subfields - CA1, CA2 and CA3. While accumulating evidence points to cellular and synaptic heterogeneity within each subfield, this heterogeneity has not ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.06.004
更新日期:2014-08-01 00:00:00
abstract::Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80800-7
更新日期:1988-02-29 00:00:00
abstract::The mitochondrial outer membrane contains a protein complex with at least eight subunits responsible for recognition and translocation of preproteins synthesized in the cytosol. Two subunits, the receptors Tom20 and Tom70, contain tetratrico peptide repeats that are thought to be involved in protein-protein interactio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00156-1
更新日期:1996-03-11 00:00:00
abstract::We report the first evidence of a mitochondrial NO synthase (mtNOS) in bird skeletal muscle. In vitro, mtNOS activity stimulated by L-arginine reduced intermyofibrillar mitochondrial oxygen uptake and ATP synthesis rates, stimulated endogenous H(2)O(2) generation, but had no effect on oxidative phosphorylation efficie...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.11.032
更新日期:2011-01-03 00:00:00
abstract::In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3beta,5alpha,6beta-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells. We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01831-7
更新日期:2000-07-28 00:00:00
abstract::Apomyoglobin is a widely used model for studying the molecular mechanisms of globular protein folding. This work aimed to analyze the effects of rigidity and length of loops linking protein secondary structure elements on the stability of the molten globule intermediate state. For this purpose, we studied folding/unfo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13905
更新日期:2020-10-01 00:00:00
abstract::We have studied the effects of 'hydrophobic mismatch' between a poly-Leu transmembrane helix (TMH) and the ER membrane using a glycosylation mapping approach. The simplest interpretation of our results is that the lumenal end of the TMH is located deeper in the membrane for both short (negative mismatch) and long (pos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02415-2
更新日期:2001-05-11 00:00:00
abstract::The eye lens protein and chaperonin, alpha-crystallin, was studied by differential scanning microcalorimetry, spectroscopy and size exclusion chromatography. The thermal transition of alpha-crystallin proceeds at Ttrs = 59.8 +/- 0.6 degrees C with an enthalpy change of delta H = 336 +/- 9 kJ per mol subunit. Disagreem...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00867-8
更新日期:1996-09-16 00:00:00
abstract::Bovine leukaemia virus (BLV) is the aetiological agent of Leukosis enzootica bovis [Viral Oncology (1980), G. Klein (Ed.) Raven Press, New York, pp. 231-238], a widely spread disease in cattle. BLV is reported as the animal model of human T-cell leukaemia virus (HLTV) which is the causative agent of adult T-cell leuka...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81798-5
更新日期:1993-07-12 00:00:00
abstract::Myometrial membranes, obtained from estrogen-dominated (day 0) rat uteri, were immunoblotted with antiserum (SG1), which recognizes the alpha subunits of both Gi1 and Gi2, with antiserum (LE2) specific for Gi2 alpha, and with I3B antiserum, specific for Gi3 alpha. The data revealed the absence of detectable levels of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80070-j
更新日期:1991-01-14 00:00:00
abstract::A human liver cDNA clone which encodes the UDP-glucose pyrophosphorylase was isolated by complementation of a bacterial galU mutant. The deduced amino acid sequence of the human enzyme comprised 508 amino acids with a calculated molecular mass of 56,950. The human enzyme significantly resembles those of potato tuber a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80213-e
更新日期:1993-08-23 00:00:00
abstract::The α-subunit of the cardiac voltage-gated sodium channel (NaV1.5) plays a central role in cardiomyocyte excitability. We have recently reported that NaV1.5 is post-translationally modified by arginine methylation. Here, we aimed to identify the enzymes that methylate NaV1.5, and to describe the role of arginine methy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.043
更新日期:2013-10-01 00:00:00
abstract::The WW domain is known as one of the smallest protein modules with a triple-stranded beta-sheet fold. Here, we present the solution structure of the second WW domain from the mouse salvador homolog 1 protein. This WW domain forms a homodimer with a beta-clam-like motif, as evidenced by size exclusion chromatography, a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.01.008
更新日期:2007-02-06 00:00:00
abstract::Oogenesis of amphibians is an atypical situation in which histone mRNA is polyadenylated. The poly(A) tract on H4 mRNA has been examined by S1 nuclease analysis. Throughout oogenesis the poly(A) tract is very short, and nonexistent on some mRNA molecules. The poly(A) tract is completely removed during maturation of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81075-9
更新日期:1985-01-28 00:00:00
abstract::Aminoacyl-tRNA synthetases, a group of enzymes catalyzing aminoacyl-tRNA formation, may possess inherent editing activity to clear mistakes arising through the selection of non-cognate amino acid. It is generally assumed that both editing substrates, non-cognate aminoacyl-adenylate and misacylated tRNA, are hydrolyzed...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.09.058
更新日期:2007-10-30 00:00:00
abstract::In the presence of ammonium sulfate the absorption spectra of a peroxidase from the fungus Arthromyces ramosus (ARP) showed that the low-spin component increased as the pH increased from 6.0 to 9.0, whereas in its absence ARP remained in the high-spin state in the pH range investigated. The crystal structure of ARP at...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01458-6
更新日期:1996-01-15 00:00:00
abstract::For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.04.035
更新日期:2012-06-04 00:00:00
abstract::Two-dimensional nuclear magnetic resonance spectroscopy (2D-NMR) was used to assign the proton resonances of ferricytochrome C553 from Desulfovibrio vulgaris Hildenborough. The spin systems of 76 out of 79 amino acids were identified by J-correlation spectroscopy (COSY and HOHAHA) in H20 and D20 and correlated by nucl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00580-7
更新日期:1996-07-01 00:00:00
abstract::Anaerobic biosynthesis of carotenoids in the purple facultative photosynthetic bacterium Rhodobacter sphaeroides was studied using mass spectrometry. We have demonstrated that (18)O from H2(18)O was incorporated into the 1-methoxy group of spheroidene and spheroidenone, the two major carotenoids produced by this bacte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00006-9
更新日期:1997-02-10 00:00:00
abstract::The hemoregulatory peptide PyroGlu-Glu-Asp-Cys-Lys (HP5b), which inhibits myelopoietic colony formation in vitro, is shown to be a sequence motif which is also part of the effector domain of Gi alpha proteins. Out of 8 synthetic peptides with sequence variations of HP5b, those with the closest similarity to the Gi alp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81106-x
更新日期:1990-08-20 00:00:00