Abstract:
:For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP fusion protein that retains functionality in bona fide Wnt activity assays, although the secreted protein is rapidly cleaved by extracellular proteases. We can show with this new tool that Wnt2b-EGFP moves along the microtubules of Wnt producing cells and that this directed movement is essential for the secretion of active Wnt protein.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Holzer T,Liffers K,Rahm K,Trageser B,Ozbek S,Gradl Ddoi
10.1016/j.febslet.2012.04.035subject
Has Abstractpub_date
2012-06-04 00:00:00pages
1638-44issue
11eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00324-9journal_volume
586pub_type
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