Transcriptomic analyses suggest that mucopolysaccharidosis patients may be less susceptible to COVID-19.

abstract：:The thioredoxin-catalyzed insulin reduction by dihydrolipoate was applied to study the 2-oxoacid: lipoate oxidoreductase activity of 2-oxoacid dehydrogenase complexes. The enzymatic and non-enzymatic mechanisms of the transfer of reducing equivalents from the complexes to free lipoic acid (alpha-lipoic acid, 6,8-thioo...

journal_title：FEBS letters

authors： Bunik V,Shoubnikova A,Loeffelhardt S,Bisswanger H,Borbe HO,Follmann H

更新日期：1995-09-04 00:00:00

abstract：:Pasteurella multocida toxin is a potent mitogen for cultured Swiss 3T3 cells where it causes an accumulation of inositol phosphates and activation of protein kinase C. The gene sequence described here coded for a 146 kDa protein. The ORF was preceded by a ribosome binding site and followed by a stem loop. There was no...

journal_title：FEBS letters

authors： Lax AJ,Chanter N,Pullinger GD,Higgins T,Staddon JM,Rozengurt E

更新日期：1990-12-17 00:00:00

abstract：:Endothelial cells play a pivotal role in the development of atherosclerosis. An 'activated' phenotype of these cells is manifested by signal transduction-dependent expression of genes encoding cytokines, pro- and anticoagulant factors, and cell adhesion molecules. In the current study we examined the effect of ouabain...

journal_title：FEBS letters

authors： Bereta J,Cohen MC,Bereta M

更新日期：1995-12-11 00:00:00

abstract：:The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized ...

journal_title：FEBS letters

authors： Tanimura S,Ohtsuka S,Mitsui K,Shirouzu K,Yoshimura A,Ohtsubo M

更新日期：1999-03-19 00:00:00

abstract：:Vibrio cholerae causes the cholera disease through secretion of cholera toxin (CT), resulting in severe diarrhoea by modulation of membrane transporters in the intestinal epithelium. Genes encoding membrane-spanning transporters identified as being differentially expressed during cholera disease in a microarray screen...

journal_title：FEBS letters

authors： Flach CF,Qadri F,Bhuiyan TR,Alam NH,Jennische E,Holmgren J,Lönnroth I

更新日期：2007-07-10 00:00:00

abstract：:The number of synthetic UTP analogues containing methyl groups in different positions of the ribose moiety were tested as substrates for T7 RNA polymerase (T7 RNAP). Two of these compounds (containing substituents in the 5' position) were shown to be weak substrates of T7 RNAP. 3'Me-UTP was neither substrate nor inhib...

journal_title：FEBS letters

authors： Tunitskaya VL,Rusakova EE,Padyukova NSh,Ermolinsky BS,Chernyi AA,Kochetkov SN,Lysov YuP,Mikhailov SN

更新日期：1997-01-06 00:00:00

abstract：:Nucleoside modifications in tRNA anticodons regulate ribosome dynamics during translation elongation and, thereby, fine-tune global protein synthesis rates. The highly conserved eukaryotic Elongator complex conducts specific C5-substitutions in tRNA wobble base uridines. It harbors two copies of each of its six indivi...

journal_title：FEBS letters

authors： Dauden MI,Jaciuk M,Müller CW,Glatt S

更新日期：2018-02-01 00:00:00

abstract：:The effects of ADP, ATP, citrate and EDTA on iron-dependent microsomal and liposomal lipid peroxidation, and on 59FeCl3 binding to the lipid membranes were measured. The aim was to test if initiation of lipid peroxidation is a site-specific mechanism requiring bound iron. In the absence of chelator, iron was bound to ...

journal_title：FEBS letters

authors： Vile GF,Winterbourn CC

更新日期：1987-05-04 00:00:00

abstract：:In the presence of ammonium sulfate the absorption spectra of a peroxidase from the fungus Arthromyces ramosus (ARP) showed that the low-spin component increased as the pH increased from 6.0 to 9.0, whereas in its absence ARP remained in the high-spin state in the pH range investigated. The crystal structure of ARP at...

journal_title：FEBS letters

authors： Kunishima N,Amada F,Fukuyama K,Kawamoto M,Matsunaga T,Matsubara H

更新日期：1996-01-15 00:00:00

abstract：:WW domains harbor substrates containing proline-rich motifs, but the substrate specificity and binding mechanism remain elusive for those WW domains less amenable for structural studies, such as human WWP2 (hWWP2). Herein we have employed multiple techniques to investigate the second WW domain (WW2) in hWWP2. Our resu...

journal_title：FEBS letters

authors： Jiang J,Wang N,Jiang Y,Tan H,Zheng J,Chen G,Jia Z

更新日期：2015-07-08 00:00:00

abstract：:The WRN protein is mutated in the chromosomally unstable Werner syndrome (WS) and the Nbs1 protein is mutated in Nijmegen breakage syndrome (NBS). The Nbs1 protein is an integral component of the M/R/N complex. Although WRN is known to interact with this complex in response to gamma-irradiation, the mechanism of actio...

journal_title：FEBS letters

authors： Cheng WH,Sakamoto S,Fox JT,Komatsu K,Carney J,Bohr VA

更新日期：2005-02-28 00:00:00

abstract：:Activation gating in KcsA is elicited by changes in intracellular proton concentration. Thompson et al. identified a charge cluster around the inner gate that plays a key role in defining proton activation in KcsA. Here, through functional and spectroscopic approaches, we confirmed the role of this charge cluster and ...

journal_title：FEBS letters

authors： Cuello LG,Cortes DM,Jogini V,Sompornpisut A,Perozo E

更新日期：2010-03-19 00:00:00

abstract：:Tomato Cf-9, a receptor-like protein (RLP), confers resistance to races of the fungal pathogen Cladosporium fulvum that express the Avr9 avirulence gene. CITRX (Cf-9-interacting thioredoxin) was previously identified in a yeast two-hybrid screen as a protein interacting with the cytoplasmic domain of Cf-9 and shown to...

journal_title：FEBS letters

authors： Nekrasov V,Ludwig AA,Jones JD

更新日期：2006-07-24 00:00:00

abstract：:The androgen-binding protein (ABP) has been purified 87,500-fold from rat testis using 4 steps of HPLC, with a yield of 14%. The molecule was 99% pure with a specific activity estimated to 16,600 pmol/mg protein. The iodinated molecule was eluted in 2 peaks in Sephacryl S300 gel filtration with a molecular mass estima...

journal_title：FEBS letters

authors： Gueant JL,Khanfri J,Gerard H,Fremont S,Gerard A,Grignon G,Nicolas JP

更新日期：1986-10-27 00:00:00

abstract：:To better understand the mechanism of intracellular Ca2+ mobilization, mouse oocytes were micro-injected with 'caged'-inositol-1,4,5 triphosphate caged-InsP3) together with the Ca2+ indicator Fluo-3 to directly induce and monitor Ca2+ redistribution. Photo-released InsP3 elicits [Ca2+]i changes exhibiting several kine...

journal_title：FEBS letters

authors： Peres A

更新日期：1990-11-26 00:00:00

abstract：:Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory p...

journal_title：FEBS letters

authors： Kairane C,Zilmer M,Mutt V,Sillard R

更新日期：1994-05-23 00:00:00

abstract：:The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, 56, 62, 67, 105 and 125 kDa. None of these complexes could be cross-linked following ribonucleas...

journal_title：FEBS letters

authors： Boix J,Habets WJ,van Venrooij WJ,Smith HC

更新日期：1990-10-15 00:00:00

abstract：:Uptake of taurine in human placental brush-border membrane vesicles was greatly stimulated in the presence of an inwardly-directed Na+ + Cl- -gradient and uphill transport of taurine could be demonstrated under these conditions. Na+ as well as Cl- were obligatory for this uptake and both ion gradients could energize t...

journal_title：FEBS letters

authors： Miyamoto Y,Balkovetz DF,Leibach FH,Mahesh VB,Ganapathy V

更新日期：1988-04-11 00:00:00

abstract：:The four-step caffeine biosynthetic pathway includes three methylation steps that utilise S-adenosyl-L-methionine (SAM) as the methyl donor. In the process SAM is converted to S-adenosyl-L-homocysteine (SAH) which in turn is hydrolysed to L-homocysteine and adenosine. Significant amounts of radioactivity from [methyl-...

journal_title：FEBS letters

authors： Koshiishi C,Kato A,Yama S,Crozier A,Ashihara H

更新日期：2001-06-15 00:00:00

abstract：:Analysis of glutamic acid decarboxylase (GDC) (EC 4.1.1.15) from Escherichia coli ATCC 11246 revealed the presence of six pyridoxal phosphates (PLPs) as well as six covalently bound pyrroloquinoline quinones (PQQs) per hexameric enzyme molecule. This is the second example of a pyridoxo-quinoprotein, suggesting that ot...

journal_title：FEBS letters

authors： van der Meer RA,Groen BW,Duine JA

更新日期：1989-03-27 00:00:00

abstract：:A high-potential iron-sulfur cluster with characteristics similar to a Rieske-type center was detected in the plasma membrane of Sulfolobus acidocaldarius by EPR spectroscopy. In the reduced form this center has g-values of gz = 2.031, gy = 1.890 and gx = 1.725 (gav = 1.88, rhombicity = 0.37) and its reduction potenti...

journal_title：FEBS letters

authors： Anemüller S,Schmidt CL,Schäfer G,Teixeira M

更新日期：1993-02-22 00:00:00

abstract：:The Ca(2+)-activated photoprotein, aequorin, contains six tryptophan residues and has a bioluminescence emission maximum at 465 nm. On converting the six tryptophan residues to phenylalanine, the mutant aequorins exhibited varied luminescence activities and spectra, but one mutant, with tryptophan-86 replaced by pheny...

journal_title：FEBS letters

authors： Ohmiya Y,Ohashi M,Tsuji FI

更新日期：1992-04-20 00:00:00

abstract：:The E-E* model for calcium pumping by the CaATPase of sarcoplasmic reticulum includes two distinct conformational states of the enzyme, E and E*. Exterior Ca2+ binds only to E and interior Ca2+ binds only to E*. Therefore, it is expected that there will be competition between the binding of calcium to the unphosphoryl...

journal_title：FEBS letters

authors： Myung J,Jencks WP

更新日期：1991-01-14 00:00:00

abstract：:We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-alpha(TNF-alpha) and cycloheximide (CHX). By affinity purification and mass spectrometry, we identified RNA polymerase II-associated protein 3 (RPAP3) as a binding protein of Monad. Overexpressio...

journal_title：FEBS letters

authors： Itsuki Y,Saeki M,Nakahara H,Egusa H,Irie Y,Terao Y,Kawabata S,Yatani H,Kamisaki Y

更新日期：2008-07-09 00:00:00

abstract：:Ca2+ release from sarcoplasmic reticulum membranes, activated by alkaline pH occurs only when EGTA is present in the release medium. Addition of very low concentrations of Ca2+ to the medium inhibits Ca2+ release. The concentration of free Ca2+ required for 50% inhibition ranges from between 5 and 20 nM in different e...

journal_title：FEBS letters

authors： Argaman A,Shoshan-Barmatz V

更新日期：1989-01-16 00:00:00

abstract：:Two genes code for calcitonin gene-related peptides (CGRPs). One expresses by tissue-specific alternate splicing calcitonin and CGRP I mRNAs, the other CGRP II mRNA. Calcitonin is the marker of sporadic or hereditary human medullary thyroid carcinoma (MTC). CGRP II expression is not well established in normal or tumor...

journal_title：FEBS letters

authors： Lasmoles F,Minvielle S,Cohen R,Guliana JM,Delehaye MC,Segond N,Calmettes C,Milhaud G,Moukhtar MS

更新日期：1990-12-17 00:00:00

abstract：:125I-labelled angiotensin II (A II) specifically binds to solubilized receptors extracted from rat isolated glomeruli using CHAPS (3-[3-( cholamidopropyl ) dimethylammonio ]-1-propanesulfonate). The yield of solubilization of the binding sites was 3.3%. Equilibrium was reached after 15-20 min and specific binding repr...

journal_title：FEBS letters

authors： Chansel D,Ardaillou N,Ardaillou R

更新日期：1984-05-21 00:00:00

abstract：:We have demonstrated over a decade ago that hepoxilins cause the release of insulin from isolated pancreatic islets of Langerhans in vitro. However, no studies are available so far to indicate whether these compounds are active in vivo. The present study is the first to our knowledge which demonstrates that hepoxilins...

journal_title：FEBS letters

authors： Pace-Asciak CR,Demin PM,Estrada M,Liu G

更新日期：1999-11-19 00:00:00

abstract：:Structural gene expression of human immunodeficiency virus type 1 (HIV-1) requires a viral regulatory protein, Rev transactivator. We investigated Rev-dependency of HIV-1 gene expression by various reporter systems. Expression of unspliced and single-spliced viral mRNAs was demonstrated to be differentially dependent ...

journal_title：FEBS letters

authors： Sakai H,Furuta RA,Tokunaga K,Kawamura M,Hatanaka M,Adachi A

更新日期：1995-05-29 00:00:00

abstract：:Interrogation of the Human Genome data for sequences related to the von Willebrand factor A-domain module identified a previously unreported 4.1 kb full-length cDNA that is predicted to encode a new member of the collagen superfamily of extracellular matrix proteins, collagen XXI. The domain organization of collagen X...

journal_title：FEBS letters

authors： Fitzgerald J,Bateman JF

更新日期：2001-09-14 00:00:00