Abstract:
:Vibrio cholerae causes the cholera disease through secretion of cholera toxin (CT), resulting in severe diarrhoea by modulation of membrane transporters in the intestinal epithelium. Genes encoding membrane-spanning transporters identified as being differentially expressed during cholera disease in a microarray screening were studied by real-time PCR, immunohistochemistry and in a CaCo-2 cell model. Two amino acid transporters, SLC7A11 and SLC6A14, were upregulated in acute cholera patients compared to convalescence. Five other transporters were downregulated; aquaporin 10, SLC6A4, TRPM6, SLC23A1 and SLC30A4, which have specificity for water, serotonin (5-HT), magnesium, vitamin C and zinc, respectively. The majority of these changes appear to be attempts of the host to counteract the secretory response. Our results also support the concept that epithelial cells are involved in 5-HT signalling during acute cholera.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Flach CF,Qadri F,Bhuiyan TR,Alam NH,Jennische E,Holmgren J,Lönnroth Idoi
10.1016/j.febslet.2007.06.001subject
Has Abstractpub_date
2007-07-10 00:00:00pages
3183-8issue
17eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00651-5journal_volume
581pub_type
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