Abstract:
:To identify the novel genes involved in lipid metabolism and lipid droplet formation that may play important roles in Hepatitis C virus (HCV) propagation, we have screened the small interfering RNA library using cell culture derived HCV (HCVcc)-infected cells. We selected and characterized the gene encoding farnesyl-diphosphate farnesyltransferase 1 (FDFT1). siRNA-mediated knockdown of FDFT1 impaired HCV replication in both subgenomic replicon and HCVcc infected cells. Moreover, YM-53601, an inhibitor of FDFT1 enzyme activity, abrogated HCV propagation. HCV infection increased FDFT1 protein level but not FDFT1 mRNA level. These results suggest that HCV may modulate FDFT1 protein level to facilitate its own propagation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Park EM,Nguyen LN,Lim YS,Hwang SBdoi
10.1016/j.febslet.2014.03.043subject
Has Abstractpub_date
2014-05-02 00:00:00pages
1813-20issue
9eissn
0014-5793issn
1873-3468pii
S0014-5793(14)00262-2journal_volume
588pub_type
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