Abstract:
:DNA enzymes are RNA-cleaving single-stranded DNA molecules. We designed DNA enzymes targeting the PB2 mRNA translation initiation (AUG) region of the influenza A virus (A/PR/8/34). The modified DNA enzymes have one or two N3'-P5' phosphoramidate bonds at both the 3'- and 5'-termini of the oligonucleotides, which significantly enhanced their nuclease resistance. These modified DNA enzymes had the same cleavage activity as the unmodified DNA enzymes, determined by kinetic analyses, and reduced influenza A virus replication by more than 99%, determined by plaque formation. These DNA enzymes are highly specific; their protective effect was not observed in influenza B virus (B/Ibaraki)-infected Madin-Darby canine kidney cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Takahashi H,Hamazaki H,Habu Y,Hayashi M,Abe T,Miyano-Kurosaki N,Takaku Hdoi
10.1016/S0014-5793(04)00073-0keywords:
subject
Has Abstractpub_date
2004-02-27 00:00:00pages
69-74issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304000730journal_volume
560pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::To identify genes whose expression is modulated by heregulin-beta1 (HRG), a regulatory polypeptide for mammary epithelial cells, we performed differential display screening of MCF-7 cell mRNA. One cDNA clone upregulated by HRG was identical to human ADP-ribosylation factor 3 (ARF3), a guanine nucleotide-binding protei...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02994-0
更新日期:2002-07-31 00:00:00
abstract::Blebs of the sarcoplasmic reticulum (SR) membrane of heart muscle cells were generated after saponin perforation of the plasma membrane followed by complete hypercontraction of the cell. Although characteristic proteins of the plasma membrane, namely the beta1-adrenoreceptor and Galphai, were stained by monoclonal ant...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02862-9
更新日期:2001-09-21 00:00:00
abstract::Mice deficient in granulocyte-macrophage colony stimulating factor (GM-CSF) develop pulmonary alveolar proteinosis (PAP). We found that bronchoalveolar lavage fluid (BALF) from 11 patients with idiopathic pulmonary alveolar proteinosis (IPAP) suppressed the growth of peripheral blood monocytes and TF-1 cells, a cell l...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01668-8
更新日期:1999-01-15 00:00:00
abstract::RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.02.052
更新日期:2012-04-05 00:00:00
abstract::Human parainfluenza virus type 3 (hPIV3) recognizes both α2,3- and α2,6-linked sialic acids, whereas human parainfluenza virus type 1 (hPIV1) recognizes only α2,3-linked sialic acids. To identify amino acid residues that confer α2,6-linked sialic acid recognition of hPIV3, amino acid residues in or neighboring the sia...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.03.036
更新日期:2015-05-08 00:00:00
abstract::Chemotherapy is a major anticancer therapeutic modality, however, multidrug resistance (MDR) is frequently observed and hinders treatment efficacy. Here, we investigated the role and potential mechanism of the long noncoding RNA (lncRNA) FENDRR in adriamycin resistance of chronic myeloid leukaemia (CML) cells. FENDRR ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13480
更新日期:2019-08-01 00:00:00
abstract::tRNAs play a central role in protein translation, acting as the carrier of amino acids. By cloning microRNAs, we unexpectedly obtained some tRNA fragments generated by tRNA cleavage in the anticodon loop. These tRNA fragments are present in many cell lines and different mouse tissues. In addition, various stress condi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.12.043
更新日期:2009-01-22 00:00:00
abstract::The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.05.004
更新日期:2007-06-12 00:00:00
abstract::Mouse spermatozoa stimulated with epidermal growth factor (EGF) or zona pellucida (ZP) experienced phosphatidylinositol 4,5-bisphosphate hydrolysis, diacylglycerol (DAG) generation and acrosomal exocytosis. The agonists showed additive effects but the action of EGF is likely to be mediated by a distinct receptor becau...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00114-o
更新日期:1995-03-06 00:00:00
abstract::Colneleic acid (9-[1'(E),3'(Z)-nonadienyloxy]-8(E)-nonenoic acid) is produced from linoleic acid by the sequential action of 9-lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemica...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00171-6
更新日期:2003-03-13 00:00:00
abstract::14-3-3s predominantly form homo-/heterodimers that are in equilibrium with corresponding monomers. Dimer/monomer equilibrium depends on the nature and phosphorylation of Ser58 of certain 14-3-3 isoforms. The structure and properties of 14-3-3 dimers are well characterized, whereas 14-3-3 monomers are less investigated...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.10.048
更新日期:2012-12-14 00:00:00
abstract::Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80661-x
更新日期:1985-05-06 00:00:00
abstract::CHO SSF3 cells grow as a suspension culture in unmodified commercial medium with only low-molecular weight ingredients. Continuous serum-free culture unexpectedly induced expression of a low dihydrofolate reductase activity in the originally dhfr- CHO cells. Nevertheless, it was possible with methotrexate to induce am...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01328-8
更新日期:1995-12-27 00:00:00
abstract::Cathelicidins are a group of myeloid antimicrobial peptide precursors found in a variety of mammalian species. Transcripts of this family show a highly conserved 5' region corresponding to the 5' untranslated region, signal peptide and propiece, and diverse 3' regions encoding structurally varied C-terminal sequences ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01310-0
更新日期:1997-11-17 00:00:00
abstract::Thymocytes previously loaded with quin 2 were rapidly permeabilized by the photon activation of erythrosin and the rate of permeabilization monitored by measuring fluorimetrically the increasing saturation of quin 2 with calcium. The extent of permeabilization was assessed also by the loss of [3H]quin 2 from the thymo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81530-2
更新日期:1987-03-23 00:00:00
abstract::A 32 kDa phospholipase A2 inhibitory protein was isolated from pig thyroid gland after calcium precipitation and fast protein liquid anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis revealed the purity of the protein. The protein activity was assessed by the inhibition of pancreatic phospholipase ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81273-0
更新日期:1988-08-01 00:00:00
abstract::32 consensus patterns for a set of functional regions and structural motifs in protein sequences were constructed. The pattern definition is heuristic and based on 11 selected steric and physicochemical properties. By comparison with these patterns, it was possible to identify, without false detection, 1532 sites in 8...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81818-6
更新日期:1989-10-23 00:00:00
abstract::The uncoupling protein-3 (UCP3) is a mitochondrial protein expressed mainly in skeletal muscle. Among several hypotheses for its physiological function, UCP3 has been proposed to prevent excessive production of reactive oxygen species. In the present study, we evaluated the effect of an oxidative stress induced by hyp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.084
更新日期:2005-06-20 00:00:00
abstract::Comparison of the phosphotyrosine-specific dephosphorylation of the autophosphorylated receptors for epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) in Swiss 3T3 cell membranes by the endogenous phosphatases revealed striking differences. EGF receptor dephosphorylation was clearly faster than P...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80352-u
更新日期:1993-10-04 00:00:00
abstract::The regulation of genes for acetylcholine receptor (AChR), myogenic factors and other muscle-specific proteins has been analyzed in experimental autoimmune myasthenia gravis (EAMG) and following denervation. The levels of the transcripts for the myogenic factors, MyoD1, myogenin and MRF4, were measured using Northern ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80089-y
更新日期:1992-03-24 00:00:00
abstract::Many biochemical reactions are confined to interfaces, such as membranes or cell walls. Despite their importance, no canonical rate laws describing the kinetics of surface-active enzymes exist. Combining the approach chosen by Michaelis and Menten 100 years ago with concepts from surface chemical physics, we here pres...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.026
更新日期:2013-09-02 00:00:00
abstract::Domain structures of porcine lung and testicular angiotensin-converting enzymes (ACE) were studied by radiation inactivation to test the hypothesis that lung ACE has two catalytic sites localized to discrete, structurally independent domains (the N- and C-domains) of approximately equal size. The minimum functional si...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80882-h
更新日期:1992-06-29 00:00:00
abstract::The molecular organisation of the Prochlorococcus marinus rnpB gene and the catalytic activity of the encoded RNA were characterised. Kinetic parameters for several pre-tRNA substrates were comparable to those from other eubacterial RNase P RNAs, although unusually high cation concentrations were required. The CCA-end...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00729-7
更新日期:1998-07-17 00:00:00
abstract::PMA-induced leukotriene C4 synthase (LTC4S) phosphorylation was investigated over a period of 8 h in a monocytic cell line (THP-1). The level of LTC4S phosphorylation was increased 3-5 fold over a 4 h period decreasing to basal levels after 8 h. This phosphorylation event was found to be specific to THP-1 cells as the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00397-x
更新日期:1999-04-16 00:00:00
abstract::Exposure of protein kinase C to low concentrations of either N-chlorosuccinimide or H2O2 resulted in rapid and parallel loss of phosphotransferase activity and phorbol ester binding. This oxidative inactivation of protein kinase C also occurred in intact cells exposed to a low concentration of H2O2. With H2O2 treatmen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81164-x
更新日期:1987-12-10 00:00:00
abstract::As a result of an experimental confluence between carcinogenesis and oxidative phosphorylation, we published in 1971 'A Unitary Hypothesis for Carcinogenesis' [(1971) J. Antibiot. Tokyo 24, 405-417]. According to this hypothesis, damaged mitochondria release mitochondrial genetic material which like that from an oncog...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81754-5
更新日期:1989-10-09 00:00:00
abstract::We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippa...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12247
更新日期:2016-07-01 00:00:00
abstract::We previously identified and purified a nucleolar phosphoprotein, nucleophosmin/B23, as a stimulatory factor for replication from the adenovirus chromatin. We show here that nucleophosmin/B23 functions as a histone chaperone protein such as nucleoplasmin, TAF-I, and NAP-I. Nucleophosmin/B23 was shown to bind to histon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02939-8
更新日期:2001-10-12 00:00:00
abstract::The antibacterial activity of many small, positively charged peptides and proteins is based on pore formation in lipid bilayers. It is here proposed to arise from an electroporation effect. This hypothesis is supported by calculations of the electrostatic potential of NK-lysin associated to a membrane. For a significa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01520-3
更新日期:1999-11-26 00:00:00
abstract::NELL1 is an extracellular protein inducing osteogenic differentiation and bone formation of osteoblastic cells. To elucidate the intracellular signaling cascade evoked by NELL1, we have shown that NELL1 protein transiently activates the MAPK signaling cascade, induces the phosphorylation of Runx2, and promotes the rap...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.12.006
更新日期:2008-01-23 00:00:00