Demonstration of cell-specific phosphorylation of LTC4 synthase.

Abstract:

:PMA-induced leukotriene C4 synthase (LTC4S) phosphorylation was investigated over a period of 8 h in a monocytic cell line (THP-1). The level of LTC4S phosphorylation was increased 3-5 fold over a 4 h period decreasing to basal levels after 8 h. This phosphorylation event was found to be specific to THP-1 cells as there was a lack of LTC4S phosphorylation in both COS-7 and K-562 cells, and was also found to be dependent on the cellular confluency. In the presence of specific protein kinase C (PKC) inhibitors, a dose-dependent inhibition of the phosphorylation of LTC4S became evident, an effect not seen with PKA and tyrosine kinase inhibitors. This represents the first direct demonstration of LTC4S phosphorylation in whole cells.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Gupta N,Nicholson DW,Ford-Hutchinson AW

doi

10.1016/s0014-5793(99)00397-x

keywords:

subject

Has Abstract

pub_date

1999-04-16 00:00:00

pages

66-70

issue

1

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(99)00397-X

journal_volume

449

pub_type

杂志文章
  • Ribosomal protein L16 binds to the 3'-end of transfer RNA.

    abstract::Escherichia coli 50 S ribosomal subunits were reconstituted with and without protein L16 present. The latter particles, although active in puromycin reaction, were unable to use CACCA-Phe as an acceptor substrate. We also found that L16 interacts directly with this oligonucleotide and, in the complex with tRNA, protec...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)80043-5

    authors: Maimets T,Remme J,Villems R

    更新日期:1984-01-23 00:00:00

  • Sodium butyrate suppresses apoptosis in human Burkitt lymphomas and murine plasmacytomas bearing c-myc translocations.

    abstract::We report that sodium butyrate, a natural product of fiber degradation by colonic bacteria, markedly suppresses c-Myc-mediated apoptosis induced in murine plasmacytomas and human Burkitt lymphomas by growth factor deprivation, but not in cell lines devoid of c-myc translocations. Attenuation of cell death is associate...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)00926-0

    authors: Alexandrov I,Romanova L,Mushinski F,Nordan R

    更新日期:1998-08-28 00:00:00

  • Molecular mechanism and physiological role of pexophagy.

    abstract::Pexophagy is a selective autophagy process wherein damaged and/or superfluous peroxisomes undergo vacuolar degradation. In methylotropic yeasts, where pexophagy has been studied most extensively, this process occurs by either micro- or macropexophagy: processes analogous to micro- and macroautophagy. Recent studies ha...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2010.01.019

    authors: Manjithaya R,Nazarko TY,Farré JC,Subramani S

    更新日期:2010-04-02 00:00:00

  • Dual role of tropomyosin on chemically modified actin filaments from skeletal muscle.

    abstract::Actin filaments copolymerized with both intact and chemically modified actin monomers restored their sliding activity when they were supplemented with tropomyosin extracted from skeletal muscle. In contrast, the ATPase activation of the copolymers was decreased when supplemented with tropomyosin. The results indicate ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(96)00218-9

    authors: Honda H,Kitano Y,Hatori K,Matsuno K

    更新日期:1996-03-25 00:00:00

  • Developmental changes in the lateral diffusion of Leydig cell membranes measured by the FRAP method.

    abstract::A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)80189-8

    authors: Zlatanov IV,Foley M,Birmingham J,Garland PB

    更新日期:1987-09-28 00:00:00

  • Structural insight into the interaction of ADP-ribose with the PARP WWE domains.

    abstract::The WWE domain is often identified in proteins associated with ubiquitination or poly-ADP-ribosylation. Structural information about WWE domains has been obtained for the ubiquitination-related proteins, such as Deltex and RNF146, but not yet for the poly-ADP-ribose polymerases (PARPs). Here we determined the solution...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2012.09.009

    authors: He F,Tsuda K,Takahashi M,Kuwasako K,Terada T,Shirouzu M,Watanabe S,Kigawa T,Kobayashi N,Güntert P,Yokoyama S,Muto Y

    更新日期:2012-11-02 00:00:00

  • Gene duplication in bovine brain myelin proteolipid and homology with related proteins.

    abstract::Analysis of the amino acid sequence of bovine brain myelin proteolipid reveals not only extensive internal homology, but also homology with portions of the myelin basic protein, the peripheral nerve myelin protein, Po, and with the small proteolipid subunit of mitochondrial ATP synthase. These results suggest that the...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(83)80732-7

    authors: Laursen RA,Samiullah M,Lees MB

    更新日期:1983-09-05 00:00:00

  • Cyclooxygenase-2 up-regulation after FLAP transfection in human adenocarcinoma cell line HT29 cl.19A.

    abstract::Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents a...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)01196-x

    authors: Battu S,Beneytout JL,Pairet M,Rigaud M

    更新日期:1998-10-16 00:00:00

  • Kinetic mechanism and ATP-binding site reactivity of p38gamma MAP kinase.

    abstract::Activated p38gamma MAP kinase exhibited significant basal ATPase activity in the absence of a kinase substrate, and addition of a phosphoacceptor substrate increased k(cat)/K(m)20-fold. AMP-PCP was competitive with ATP binding and non-competitive with phosphoacceptor substrate binding. The nucleotide binding site affi...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)01488-x

    authors: Fox T,Fitzgibbon MJ,Fleming MA,Hsiao HM,Brummel CL,Su MS

    更新日期:1999-11-19 00:00:00

  • Effect of temperature on the role of Hsp104 and trehalose in barotolerance of Saccharomyces cerevisiae.

    abstract::We have studied the effect of temperature on the contribution of Hsp104 and trehalose to barotolerance using mutants deficient in Hsp104 and trehalose synthesis. When compared with a corresponding wild type strain, mutants of Hsp104 did not show temperature dependent barotolerance when the incubation temperature durin...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(97)01141-1

    authors: Iwahashi H,Obuchi K,Fujii S,Komatsu Y

    更新日期:1997-10-13 00:00:00

  • Alternative splicing of the human Shaker K+ channel beta 1 gene and functional expression of the beta 2 gene product.

    abstract::Mammalian voltage-activated Shaker K+ channels associate with at least three cytoplasmic proteins: Kv beta 1, Kv beta 2 and Kv beta 3. These beta subunits contain variable N-termini, which can modulate the inactivation of Shaker alpha subunits, but are homologous throughout an aldo-keto reductase core. Human and ferre...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)00785-8

    authors: McCormack K,McCormack T,Tanouye M,Rudy B,Stühmer W

    更新日期:1995-08-14 00:00:00

  • Decrease of cellular ATP by dihexanoylglycerol may limit responses to protein kinase C activation.

    abstract::1,2-sn-Dihexanoylglycerol (HHG) reduced the ATP content of HL-60 cells. This concentration-related (10-100 microM) effect reached a maximum of over 90%, was enantiomerically specific and not accompanied by release of lactate dehydrogenase. Oleoylacetylglycerols (3-100 microM) had no effect on ATP levels while phorbol ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)80036-4

    authors: Dawson J,Thompson NT,Bonser RW,Hodson HF,Garland LG

    更新日期:1987-04-06 00:00:00

  • Complete amino acid sequence of human seminal plasma beta-inhibin. Prediction of post Gln-Arg cleavage as a maturation site.

    abstract::The complete sequence of a 94 amino acid human seminal plasma polypeptide exhibiting inhibin-like activity is presented. This molecule, called beta-inhibin, selectively and specifically suppresses the release of pituitary FSH in vivo as well as in vitro. It does not affect the secretion of LH. Such a novel acidic prot...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)80766-8

    authors: Seidah NG,Arbatti NJ,Rochemont J,Sheth AR,Chrétien M

    更新日期:1984-10-01 00:00:00

  • Antithrombin Glasgow, 393 Arg to His: a P1 reactive site variant with increased heparin affinity but no thrombin inhibitory activity.

    abstract::Antithrombin Glasgow is a hereditary abnormal antithrombin that has lost thrombin inhibitory activity. It was isolated from the plasma of a 41-year-old male with a history of thrombotic events. Antithrombin Glasgow was purified from plasma using heparin-Sepharose chromatography at pH 7.4 eluting with increasing concen...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(88)80841-x

    authors: Owen MC,Beresford CH,Carrell RW

    更新日期:1988-04-25 00:00:00

  • Metabolic and signaling properties of an Itpk gene family in Glycine max.

    abstract::We have cloned and characterized four Itpk genes from soybean. All four recombinant Itpk proteins showed canonical Ins(1,3,4)P3 5/6-kinase activity, but a kinetic analysis raised questions about its biological significance. Instead, we provide evidence that one alternative biological role for soybean Itpks is to inter...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2008.04.054

    authors: Stiles AR,Qian X,Shears SB,Grabau EA

    更新日期:2008-06-11 00:00:00

  • A preferential pole for exocytosis in cultured chromaffin cells revealed by confocal microscopy.

    abstract::Histological studies suggest that adrenal medulla chromaffin cells in situ are polarised, but functional evidence is lacking. We present here the first demonstration for polarisation of exocytosis in isolated, spherical, bovine chromaffin cells. Cells were stimulated with 70 mM K(+) to cause a marked enhancement of ca...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)01115-1

    authors: Cuchillo-Ibáñez I,Michelena P,Albillos A,García AG

    更新日期:1999-10-01 00:00:00

  • The sodium ion pumping oxaloacetate decarboxylase of Klebsiella pneumoniae. Metal ion content, inhibitors and proteolytic degradation studies.

    abstract::Oxaloacetate decarboxylase of Klebsiella pneumoniae was shown to contain between 0.6 and 1.0 mol zinc per mol enzyme in different preparations. The decarboxylase activity was completely abolished after 15 min incubation with 1 mM Hg(NO3)2 in phosphate buffer, while the activity decreased only 20% if the incubation was...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80165-d

    authors: Dimroth P,Thomer A

    更新日期:1992-03-23 00:00:00

  • Targeted cleavage of HIV-1 envelope gene by a DNA enzyme and inhibition of HIV-1 envelope-CD4 mediated cell fusion.

    abstract::With the ultimate aim of developing an effective antiviral strategy against HIV-1, a mono-DNA enzyme possessing the 10-23 catalytic motif [Santoro and Joyce (1997) Proc. Natl. Acad. Sci. USA 94, 4264-4266] was synthesized against the HIV-1 envelope gene. We tested the in vitro cleavage efficiency of the 178 bp long tr...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)00799-6

    authors: Dash BC,Harikrishnan TA,Goila R,Shahi S,Unwalla H,Husain S,Banerjea AC

    更新日期:1998-07-24 00:00:00

  • Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc1 site of the mitochondrial respiratory chain.

    abstract::Antimycin, 2-nonyl-4-hydroxyquinoline N-oxide and funiculosin induce O.2(-) generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3-dimercaptopropanol inhibit O.2(-) generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulate...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(83)80200-2

    authors: Ksenzenko M,Konstantinov AA,Khomutov GB,Tikhonov AN,Ruuge EK

    更新日期:1983-05-02 00:00:00

  • Minimal functional size of porcine lung and testicular angiotensin-converting enzymes deduced from radiation inactivation analysis. Interaction of two highly homologous domains in somatic isoenzyme.

    abstract::Domain structures of porcine lung and testicular angiotensin-converting enzymes (ACE) were studied by radiation inactivation to test the hypothesis that lung ACE has two catalytic sites localized to discrete, structurally independent domains (the N- and C-domains) of approximately equal size. The minimum functional si...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80882-h

    authors: Sakaguchi H,Hirose S,Kume T,Hagiwara H

    更新日期:1992-06-29 00:00:00

  • A large conductance ion channel in the nuclear envelope of a higher plant cell.

    abstract::To detect and characterize ion channel activity in the nuclear envelope of a higher plant cell, we performed patch clamp experiments on nuclei isolated from coconut endosperm cells and on giant liposomes containing nuclear envelope fragments prepared from the same cells. An ion channel exhibiting a number of conductan...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80290-w

    authors: Matzke AJ,Behensky C,Weiger T,Matzke MA

    更新日期:1992-05-04 00:00:00

  • Phosphorylation of the L-type calcium channel beta subunit is involved in beta-adrenergic signal transduction in canine myocardium.

    abstract::Cyclic AMP-mediated phosphorylation of calcium channel subunits was studied in vitro and in vivo in preparations from dog heart. Calcium channels in native cardiac membranes were phosphorylated by cAMP-dependent protein kinase (PKA) solubilized with digitonin and subsequently immunoprecipitated using a polyclonal anti...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80733-b

    authors: Haase H,Karczewski P,Beckert R,Krause EG

    更新日期:1993-12-06 00:00:00

  • Direct protein-protein interactions and substrate channeling between cellular retinoic acid binding proteins and CYP26B1.

    abstract::Cellular retinoic acid binding proteins (CRABPs) bind all-trans-retinoic acid (atRA) tightly. This study aimed to determine whether atRA is channeled directly to cytochrome P450 (CYP) CYP26B1 by CRABPs, and whether CRABPs interact directly with CYP26B1. atRA bound to CRABPs (holo-CRABP) was efficiently metabolized by ...

    journal_title:FEBS letters

    pub_type: 信件

    doi:10.1002/1873-3468.12303

    authors: Nelson CH,Peng CC,Lutz JD,Yeung CK,Zelter A,Isoherranen N

    更新日期:2016-08-01 00:00:00

  • Pseudovitamin B(12) is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions.

    abstract::We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B(12) activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear ma...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.09.012

    authors: Santos F,Vera JL,Lamosa P,de Valdez GF,de Vos WM,Santos H,Sesma F,Hugenholtz J

    更新日期:2007-10-16 00:00:00

  • Solution structure of an atypical WW domain in a novel beta-clam-like dimeric form.

    abstract::The WW domain is known as one of the smallest protein modules with a triple-stranded beta-sheet fold. Here, we present the solution structure of the second WW domain from the mouse salvador homolog 1 protein. This WW domain forms a homodimer with a beta-clam-like motif, as evidenced by size exclusion chromatography, a...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.01.008

    authors: Ohnishi S,Güntert P,Koshiba S,Tomizawa T,Akasaka R,Tochio N,Sato M,Inoue M,Harada T,Watanabe S,Tanaka A,Shirouzu M,Kigawa T,Yokoyama S

    更新日期:2007-02-06 00:00:00

  • C1qTNF-related protein-6 mediates fatty acid oxidation via the activation of the AMP-activated protein kinase.

    abstract::C1qTNF-related proteins (CTRPs) are involved in diverse processes including metabolism, inflammation host defense, apoptosis, cell differentiation, autoimmunity, hibernation, and organogenesis. However, the physiological role of CTRP6 remains poorly understood. Here we demonstrate that the globular domain of CTRP6 med...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2010.01.040

    authors: Lee W,Kim MJ,Park EJ,Choi YJ,Park SY

    更新日期:2010-03-05 00:00:00

  • Anti-cytokine autoantibodies are ubiquitous in healthy individuals.

    abstract::Anti-cytokine autoantibodies in healthy individuals have been widely reported but the occurrence is variable and inconstant. We hypothesized that cytokine-binding in vivo may explain their variable and infrequent detection. Therefore, we focused on the detection of the cytokine-autoantibody complexes and found that an...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.04.029

    authors: Watanabe M,Uchida K,Nakagaki K,Kanazawa H,Trapnell BC,Hoshino Y,Kagamu H,Yoshizawa H,Keicho N,Goto H,Nakata K

    更新日期:2007-05-15 00:00:00

  • Molecular cloning in yeast by in vivo homologous recombination of the yeast putative alpha1 subunit of the voltage-gated calcium channel.

    abstract::Saccharomyces cerevisiae has only one gene encoding a putative voltage-gated Ca2+ channel pore-forming subunit, CCH1, which is not possible to be cloned by conventional molecular cloning techniques using Escherichia coli. Here, we report the successful cloning of CCH1 in yeast by in vivo homologous recombination witho...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2004.09.021

    authors: Iida K,Tada T,Iida H

    更新日期:2004-10-22 00:00:00

  • Downregulation of collagen synthesis in fibroblasts within three-dimensional collagen lattices involves transcriptional and posttranscriptional mechanisms.

    abstract::Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiment...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80006-g

    authors: Eckes B,Mauch C,Hüppe G,Krieg T

    更新日期:1993-03-01 00:00:00

  • Phosphotyrosine-containing proteins in Dictyostelium discoideum.

    abstract::Phosphotyrosine-containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and immunoprecipitation using a monoclonal anti-phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205-220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(90)81007-b

    authors: Schweiger A,Mihalache O,Muhr A,Adrian I

    更新日期:1990-07-30 00:00:00