Hyperoxia-mediated oxidative stress increases expression of UCP3 mRNA and protein in skeletal muscle.

abstract：:Affinity labeling studies have identified several conserved tyrosine residues in the alpha subunit of the nicotinic acetylcholine receptor (alpha Y93, alpha Y190, and alpha Y198) as being in or near the ligand binding site. Mutagenesis studies from several laboratories have shown that substitution of phenylalanine for...

journal_title：FEBS letters

authors： Aylwin ML,White MM

更新日期：1994-07-25 00:00:00

abstract：:The ATP synthase capacity of rat heart myocytes can be measured in sonicates of cultured cardiomyocytes. In these cells, transitions in ATP synthase capacity occur on changing to the anoxic or uncoupled state (drop in ATP synthase capacity of over 40%) or on electrically stimulating the cells to contract (rise of 70%)...

journal_title：FEBS letters

authors： Das AM,Harris DA

更新日期：1989-10-09 00:00:00

abstract：:Understanding protein beta structures has been hindered by the challenge of designing small, well-folded β-sheet systems. A β-capping motif was previously designed to help solve this problem, but not without limitations, as the termini of this β-cap were not fully available for chain extension. Combining Coulombic sid...

journal_title：FEBS letters

authors： Anderson JM,Kier BL,Shcherbakov AA,Andersen NH

更新日期：2014-12-20 00:00:00

abstract：:The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the meth...

journal_title：FEBS letters

authors： Ellermann J,Kobelt A,Pfaltz A,Thauer RK

更新日期：1987-08-17 00:00:00

abstract：:MicroRNAs mainly inhibit coding genes and long non-coding RNA expression. Here, we report that hsa-miR-125b and oncogene SIRT7/oncogenic long non-coding RNA MALAT1 were inversely expressed in bladder cancer. Hsa-miR-125b mimic down-regulated, whereas hsa-miR-125b inhibitor up-regulated the expression of SIRT7 and MALA...

journal_title：FEBS letters

authors： Han Y,Liu Y,Zhang H,Wang T,Diao R,Jiang Z,Gui Y,Cai Z

更新日期：2013-11-29 00:00:00

abstract：:c-Myc is a transcriptional regulator involved in carcinogenesis through its role in growth control and cell cycle progression. Here we attempt to relate its role in stimulating the G1-S transition to the ability to affect functioning of key cell cycle regulators, and we focus on how its property of modulating transcri...

journal_title：FEBS letters

authors： Nasi S,Ciarapica R,Jucker R,Rosati J,Soucek L

更新日期：2001-02-16 00:00:00

abstract：:Sucrose synthase (SuSy) plays an important role in sucrose degradation and occurs both as a soluble and as a membrane-associated enzyme in higher plants. We show that membrane association can vary in vivo in response to gravistimulation, apparently involving SuSy dephosphorylation, and is a reversible process in vitro...

journal_title：FEBS letters

authors： Winter H,Huber JL,Huber SC

更新日期：1997-12-29 00:00:00

abstract：:The calcitonin receptor (CTR) gene in human osteoclasts formed in a human bone marrow cell culture system was examined by reverse transcription-polymerase chain reaction (RT-PCR). The RT-PCR results indicated that the 5'-untranslated region (5'UTR) was different between CTR mRNAs in human osteoclasts and in a mammary ...

journal_title：FEBS letters

authors： Nishikawa T,Ishikawa H,Yamamoto S,Koshihara Y

更新日期：1999-09-24 00:00:00

abstract：:Development of targeted therapies for triple-negative breast cancer (TNBC, a more aggressive subtype) is an unmet medical need. We analyzed data from 887 patients with invasive breast cancer and observed that increased Wnt and histone deacetylase (HDAC) activities are associated with estrogen receptor 1 (ESR1) and pro...

journal_title：FEBS letters

authors： Sulaiman A,Sulaiman B,Khouri L,McGarry S,Nessim C,Arnaout A,Li X,Addison C,Dimitroulakos J,Wang L

更新日期：2016-12-01 00:00:00

abstract：:A hybrid protein from Neisseria meningitidis, which contains both a peroxiredoxin and a glutaredoxin domain, has been isolated. The enzyme was active in the reduction of various peroxides and dehydroascorbate in the presence of reduced glutathione. These findings suggest that both the peroxiredoxin and glutaredoxin do...

journal_title：FEBS letters

authors： Rouhier N,Jacquot JP

更新日期：2003-11-06 00:00:00

abstract：:We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery...

journal_title：FEBS letters

authors： Hasan MR,Jin M,Matsushima K,Miyamoto S,Kotani S,Nakagawa H

更新日期：2006-06-12 00:00:00

abstract：:Crosslinking of histones in mouse liver nuclei and extended chromatin with a bifunctional reagent leads to the formation of H1-H1o heterodimers as well as H1o-H1o homodimers. H1o can be also crosslinked to the core histones. Thus, the location of histone H1o within the basic repeating chromatin structure seems to be a...

journal_title：FEBS letters

authors： Dashkevich VK,Nikolaev LG,Zlatanova JS,Glotov BO,Severin ES

更新日期：1983-07-25 00:00:00

abstract：:Alix and its homologs are involved in various phenomena such as endosomal protein-sorting and adaptation to stress conditions. In this study, we found that development of Dictyostelium discoideum Alix (DdAlix) deletion mutant (alx-) cells was impaired in alkaline pH environments. The fruiting body formation efficiency...

journal_title：FEBS letters

authors： Ohkouchi S,Saito H,Aruga F,Maeda T,Shibata H,Maki M

更新日期：2005-03-14 00:00:00

abstract：:Cellular homeostasis is linked tightly to mitochondrial functions. Some damage to mitochondrial proteins and nucleic acids can lead to the depolarization of the inner mitochondrial membrane, thereby sensitizing impaired mitochondria for selective elimination by autophagy. Mitochondrial dysfunction is one of the key as...

journal_title：FEBS letters

authors： Tanaka A

更新日期：2010-04-02 00:00:00

abstract：:We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate tr...

journal_title：FEBS letters

authors： Yoon JH,Lee HS,Kim TH,Woo GH,Kim CY

更新日期：2000-06-02 00:00:00

abstract：:Partial amino acid sequence was obtained from the massive myofibrillar protein nebulin. This consists of repeating motifs of about 35 residues and super-repeats of 7 x 35 = 245 residues. The repeat-motifs are likely to be largely alpha-helical and to interact with both actin and tropomyosin in thin filaments. Nebulin ...

journal_title：FEBS letters

authors： Labeit S,Gibson T,Lakey A,Leonard K,Zeviani M,Knight P,Wardale J,Trinick J

更新日期：1991-05-06 00:00:00

abstract：:The Candida utilis QCR7 gene encoding subunit VII of the ubiquinol-cytochrome c oxidoreductase was isolated by functional complementation of the Saccharomyces cerevisiae subunit VII-null mutant. Several other subunit VII homologues as well as homologues for subunit VIII were identified by screening the GenBank databas...

journal_title：FEBS letters

authors： Boumans H,Berden JA,Grivell LA

更新日期：1995-07-10 00:00:00

abstract：:A 122 nt uridine-rich sequence (URE) in the Na(+)/glucose cotransporter (SGLT1) mRNA 3'-untranslated region is critical for cAMP-dependent message stabilization. Its function was investigated in LLC-PK(1) cells stably expressing beta-globin reporter transcripts. Insertion of the SGLT1 URE downstream from an unrelated ...

journal_title：FEBS letters

authors： Loflin P,Lever JE

更新日期：2001-03-16 00:00:00

abstract：:The functional molecular masses of the vacuolar membrane H+-ATPase in Saccharomyces cerevisiae under two kinetic conditions for ATP hydrolysis were measured by radiation inactivation. When vacuolar membrane vesicles were exposed to gamma-rays from 60Co, the activities catalyzing a single-cycle and multi-cycles of ATP ...

journal_title：FEBS letters

authors： Hirata R,Ohsumi Y,Anraku Y

更新日期：1989-02-27 00:00:00

abstract：:Ribosomes are ribozymes exerting substrate positioning and promoting substrate-mediated catalysis. Peptide-bonds are formed within a symmetrical region, thus suggesting that ribosomes evolved by gene-fusion. Remote interactions dominate substrate positioning at stereochemistry suitable for peptide-bond formation and e...

journal_title：FEBS letters

authors： Baram D,Yonath A

更新日期：2005-02-07 00:00:00

abstract：:Annexin I (AnxI) is a calcium-dependent membrane binding protein which has been implicated in various physiological activities. The region of the chicken anxI cDNA encoding the first 130 amino terminal residues was cloned by reverse transcription PCR in order to determine the relationship of its variable amino-termina...

journal_title：FEBS letters

authors： Sidis Y,Horseman ND

更新日期：1993-08-30 00:00:00

abstract：:In spite of numerous studies, there appears to be no consensus regarding the orientation and aggregation state of membrane-bound melittin. We report here the restricted environment of the sole tryptophan residue in membrane-bound melittin using environment-induced effects on the rates of solvent relaxation. When incor...

journal_title：FEBS letters

authors： Chattopadhyay A,Rukmini R

更新日期：1993-12-13 00:00:00

abstract：:A rapidly maturing variant of the red fluorescent protein DsRed was optimized for bacterial expression by random mutagenesis. The brightest variant contains six mutations, two of which (S4T and a silent mutation in codon 2) explain most of the fluorescence enhancement. The novel variants are expressed at 9-60-fold hig...

journal_title：FEBS letters

authors： Sörensen M,Lippuner C,Kaiser T,Misslitz A,Aebischer T,Bumann D

更新日期：2003-09-25 00:00:00

abstract：:A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads t...

journal_title：FEBS letters

authors： Hunt JV,Bailey JR,Schultz DL,McKay AG,Mitchinson MJ

更新日期：1994-08-08 00:00:00

abstract：:Three site-directed mutants of human epidermal growth factor, Leu-26----Gly, Leu-47----Ala, and Ile-23----Thr, were examined for their ability to stimulate the protein-tyrosine kinase activity of the epidermal growth factor receptor. The receptor binding affinities of the mutant growth factors were 20- to 50-fold lowe...

journal_title：FEBS letters

authors： Matsunami RK,Campion SR,Niyogi SK,Stevens A

更新日期：1990-05-07 00:00:00

abstract：:The substrate peptide bond cleaved by cathepsins B and L is determined not by the amino acid contributing the carboxyl group to this bond as in the case of serine proteases but rather by the presence of a neighboring amino acid with a large hydrophobic side chain. From a study of the inhibitory potency in a series, Cb...

journal_title：FEBS letters

authors： Kirschke H,Wikstrom P,Shaw E

更新日期：1988-02-08 00:00:00

abstract：:Analyzing the primary structure we predicted that calponin may interact with phospholipids. In order to check this suggestion we investigated the interaction of calponin with phospholipids by ultracentrifugation, light scattering, vesicles leakage and differential scanning calorimetry. In agreement with our prediction...

journal_title：FEBS letters

authors： Bogatcheva NV,Gusev NB

更新日期：1995-09-04 00:00:00

abstract：:We have found that the sign of phototaxis, i.e. the direction of phototactic migration either towards or away from the light source, of wild-type Chlamydomonas reinhardtii depends on its photosynthetic activity. This accounts for the frequently reported transient positive phototaxis that precedes a negative phototaxis...

journal_title：FEBS letters

authors： Takahashi T,Watanabe M

更新日期：1993-12-28 00:00:00

abstract：:Very long-chain fatty acids (VLCFAs) have a variety of physiological functions and are related to numerous disorders. The key step of VLCFA elongation is catalyzed by members of the elongase family, ELOVLs. Mammals have seven ELOVLs (ELOVL1-7), yet none of them has been purified and analyzed. In the presented study we...

journal_title：FEBS letters

authors： Naganuma T,Sato Y,Sassa T,Ohno Y,Kihara A

更新日期：2011-10-20 00:00:00

abstract：:We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromatography. Following r...

journal_title：FEBS letters

authors： Cowley DJ,Mackin RB

更新日期：1997-02-03 00:00:00