Abstract:
:RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of RBM5 is indispensable for its ability to interact with DHX15. Strikingly, we find that RBM5 stimulates the helicase activity of DHX15 in a G patch domain-dependent manner in vitro. Helicase activities play critical roles in modulating pre-mRNA splicing. Our findings thus suggest a new mechanism underlying the regulatory roles of RBM5 in pre-mRNA splicing.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Niu Z,Jin W,Zhang L,Li Xdoi
10.1016/j.febslet.2012.02.052subject
Has Abstractpub_date
2012-04-05 00:00:00pages
977-83issue
7eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00177-9journal_volume
586pub_type
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