Abstract:
:Epidermal growth factor (EGF) induces transformed phenotypes in EGF receptor-overexpressing NIH3T3 (ER12) cells. Tyrosine kinase inhibitors such as erbstatin and its stable analogue methyl 2,5-dihydroxycinnamate inhibited the EGF-induced phenotypic changes in these cells; while 5'-O-methylerbstatin, an inactive analogue, did not. Methyl 2,5-dihydroxycinnamate inhibited intracellular tyrosine kinase activity in EGF-treated ER12 cells. Methyl 2,5-dihydroxycinnamate also delayed the EGF-induced DNA synthesis from the quiescent phase ER12 cells without showing irreversible cytotoxicity. It inhibited the DNA synthesis most efficiently at the early G1 phase. Thus, tyrosine kinase inhibitors may modify malignant phenotypes in EGF receptor-overexpressing neoplasms.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Umezawa K,Sugata D,Yamashita K,Johtoh N,Shibuya Mdoi
10.1016/0014-5793(92)81491-4keywords:
subject
Has Abstractpub_date
1992-12-21 00:00:00pages
289-92issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(92)81491-4journal_volume
314pub_type
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