Abstract:
:In a wild-type strain of Saccharomyces cerevisiae, cadmium induces the activities of both gamma-glutamyl transferase (gamma-GT) and glutathione transferase 2 (Gtt2). However, Gtt2 activity did not increase under gamma-GT or Ycf1 deficiencies, suggesting that the accumulation of glutathione-cadmium in the cytosol inhibits Gtt2. On the other hand, the balance between the cytoplasmic and vacuolar level of glutathione seems to regulate gamma-GT activity, since this enzyme was not activated in a gtt2 strain. Taken together, these results suggest that gamma-GT and Gtt2 work together to remove cadmium from the cytoplasm, a crucial mechanism for metal detoxification that is dependent on glutathione.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Adamis PD,Mannarino SC,Eleutherio ECdoi
10.1016/j.febslet.2009.03.066subject
Has Abstractpub_date
2009-05-06 00:00:00pages
1489-92issue
9eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00263-4journal_volume
583pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::LIM-homeodomain (LIM-HD) proteins are evolutionary conserved developmental transcription factors. LIM-HD Lmx1a and Lmx1b orchestrate complex temporal and spatial gene expression of the dopaminergic pathway, and evidence shows they are also involved in adult neuronal homeostasis. In this review, the multiple roles play...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2015.10.020
更新日期:2015-12-21 00:00:00
abstract::The insulin receptor family consists of the homologous tyrosine kinase receptors, insulin receptor (IR), insulin-like growth factor 1 receptor (IGF1R) and insulin receptor-related receptor. The three-dimensional structures of the tyrosine kinase domain of the IR and the first three extracellular domains (L1, Cys-rich ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01509-9
更新日期:1998-12-18 00:00:00
abstract::The 27 kDa platelet membrane protein (Gn27) that binds [alpha-32P]GTP on nitrocellulose blots of SDS-polyacrylamide gels [(1987) Biochem. J. 245, 617-620] was compared with other low molecular mass GTP-binding proteins. Platelet membranes also contained 21 kDa proteins that bound anti-ras p21 antibody and 22-23 kDa pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80194-7
更新日期:1988-09-12 00:00:00
abstract::Diphtheria toxin fragment A is able to inhibit protein synthesis in the eukaryotic cell by ADP-ribosylating the diphthamide residue of elongation factor-2 (EF-2) [(1980) J. Biol. Chem. 255, 10710-10720]. The reaction requires NAD as ADP-ribose donor. This work reports on the capacity of an NAD analog, the nicotinamide...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80006-5
更新日期:1985-10-28 00:00:00
abstract::The surprising result that the spanning domain causes retention of proteins in the Golgi stack poses the question as to the actual mechanism. Here we present a simple model that might have general applicability. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80906-b
更新日期:1993-09-06 00:00:00
abstract::Poly(A)-binding protein (PABP) mRNA is subject to autoregulation through a 61 nucleotides long A-rich sequence in its 5' untranslated region (UTR). Here, we show that this mode of regulation is exerted in a cell type-specific manner. Thus, overexpression of PABP in mouse NIH 3T3 fibroblasts represses the translation o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01039-x
更新日期:1999-08-27 00:00:00
abstract::32 consensus patterns for a set of functional regions and structural motifs in protein sequences were constructed. The pattern definition is heuristic and based on 11 selected steric and physicochemical properties. By comparison with these patterns, it was possible to identify, without false detection, 1532 sites in 8...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81818-6
更新日期:1989-10-23 00:00:00
abstract::N-Oligosaccharyltransferase catalyzes the N-glycosylation of asparagine residues of nascent polypeptide chains in the endoplasmic reticulum, a pathway highly conserved in all eukaryotes. An enzymatically active complex was isolated from microsomal membranes from Saccharomyces cerevisiae, which is composed of four prot...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00356-4
更新日期:1994-05-09 00:00:00
abstract::The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate G...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.02.071
更新日期:2006-04-03 00:00:00
abstract::Induction of differentiation in HL-60 and U-937 leukemic cell lines, resulted in 1.5-10-fold increase in 45Ca2+ uptake. The increased 45Ca2+ uptake in the differentiating cells was inhibited by verapamil, cromolyn and amiloride. Elevation in Ca2+ uptake in differentiating cells was also demonstrated using the fluoresc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80065-6
更新日期:1988-08-29 00:00:00
abstract::The informational content of the primary structure of thymopoietin (TP) is investigated using the informational spectrum method (ISM). We show that the sequence of TP shares common information with the sequences of long postsynaptic snake neurotoxins, although no apparent similarity was found among their primary struc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80561-1
更新日期:1988-10-24 00:00:00
abstract::The gas phase of the soil plays an important role in plant growth and development. We investigated the effect of rhizospheric NO as a signalling compound for N uptake of beech roots. Following exposure to NO, ammonium and glutamine uptake into roots were determined using (15)N-labelling, and gene expression of selecte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.07.052
更新日期:2009-09-03 00:00:00
abstract::Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.06.019
更新日期:2013-08-02 00:00:00
abstract::Human uncoupling protein 3 (UCP3) has two RNA transcripts that arise from the differential processing of the same gene product. One encodes the full length protein (UCP3L) while the other encodes a truncated version (UCP3S) lacking the sixth membrane spanning domain. The roles of the two isoforms are not known, but a ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01688-9
更新日期:2000-01-14 00:00:00
abstract::We report a rapid and sensitive colorimetric approach to quantitate the amount of glucose transporters exposed at the surface of intact cells, using L6 muscle cells expressing GLUT4 containing an exofacial myc epitope. Unstimulated cells exposed to the surface 5 fmol GLUT4myc per mg protein. This value increased to 10...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00423-2
更新日期:1998-05-08 00:00:00
abstract::The yeast PCK1 gene coding for phosphoenolpyruvate carboxykinase (PEPCK) was isolated by functional complementation of pck1 strains from S. cerevisiae. Only one copy of the gene was found per haploid yeast genome. An RNA of about 2 kb which hybridized with a DNA probe internal to the PCK1 gene was found only in cells ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81682-5
更新日期:1989-12-04 00:00:00
abstract::The off rate from the first calcium-binding site of the C2A domain of synaptotagmin I, a putative calcium receptor in neurotransmitter release, has been determined by 15N-nuclear magnetic resonance relaxation dispersion measurements. The exchange rate was obtained by fitting the dependence of the transverse relaxation...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02508-5
更新日期:2002-04-10 00:00:00
abstract::Suppressors of cytokine signaling (SOCS) proteins possess common structures, a SOCS box at the C-terminus and a SH2 domain at their center. These suppressors are inducible in response to cytokines and act as negative regulators of cytokine signaling. The ASB proteins also contain the SOCS box and the ankyrin repeat se...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02829-0
更新日期:2001-09-14 00:00:00
abstract::Escherichia coli 50 S ribosomal subunits were reconstituted with and without protein L16 present. The latter particles, although active in puromycin reaction, were unable to use CACCA-Phe as an acceptor substrate. We also found that L16 interacts directly with this oligonucleotide and, in the complex with tRNA, protec...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80043-5
更新日期:1984-01-23 00:00:00
abstract::Recently, it has been shown that really interesting new gene (RING)-in between ring finger (IBR)-RING domain-containing proteins, such as Parkin and Parc, are E3 ubiquitin ligases and are involved in regulation of apoptosis. In this report, we show that p53-inducible RING-finger protein (p53RFP), a p53-inducible E3 ub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.09.105
更新日期:2006-02-06 00:00:00
abstract::The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.12.023
更新日期:2015-01-30 00:00:00
abstract::The primary structure of rhodopsin from the octopus Paroctopus defleini has been determined by parallel analysis of the protein and corresponding cDNA. The amino acid sequence is most similar to the recently cloned Drosophila opsins. Similarities to bovine and human opsins are also evident. The transmembrane topology ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80388-0
更新日期:1988-05-09 00:00:00
abstract::T cells are stimulated by the engagement of antigen, cytokine, pathogen, and hormone receptors. While research performed over many years has focused on deciphering the molecular components of these pathways, recent data underscore the importance of the metabolic environment in conditioning responses to receptor engage...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12849
更新日期:2017-10-01 00:00:00
abstract::Cleavage of the envelope glycoprotein precursor gp160 of HIV-1 is a prerequisite for the infectivity of HIV-1, and occurs at least in part before gp160 reaches the cell surface. Kexin/subtilisin-related endopeptidases are proposed enzyme candidates for this intracellular processing. In this study, we reveal the possib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01612-3
更新日期:1999-01-08 00:00:00
abstract::Intrinsically unstructured proteins (IUPs) are common in various proteomes and occupy a unique structural and functional niche in which function is directly linked to structural disorder. The evidence that these proteins exist without a well-defined folded structure in vitro is compelling, and justifies considering th...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.03.072
更新日期:2005-06-13 00:00:00
abstract::In thrombin-stimulated platelets alpha IIb beta 3 integrin engagement triggers both phosphatidylinositol 3',4'-bisphosphate synthesis and calpain activation. We checked the possible involvement of calpains in phosphatidylinositol 3-kinase signalling pathway using a cell permeant specific inhibitor of calpains, calpept...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00079-3
更新日期:1997-03-03 00:00:00
abstract::The oxysterols, 7beta-hydroxycholesterol and 7-ketocholesterol, are involved in the cytotoxicity of oxidized LDL. To elucidate their molecular mechanisms, the human promonocytic leukemia cells U937 and U4 were used. U4 cells overexpressing Bcl-2 were obtained by transfection of U937 cells. 7Beta-hydroxycholesterol and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01473-7
更新日期:1997-12-15 00:00:00
abstract::Chromatography of the soluble porcine lymphocyte phospholipase C on cellulose phosphate resolves the activity into two species. An HPLC method is described for separating the enzyme products, Ins 1,2 greater than P and Ins 1P. Use of these methods reveals that the two iso-enzymes liberate, with a high degree of reprod...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81380-1
更新日期:1986-08-11 00:00:00
abstract::A series of 9-beta-D-xylofuranosyladenine (xyloA or xyloadenosine) substituted analogs of 2-5A core trimer and tetramer were examined for their ability to be degraded by the 2',5'-phosphodiesterase activity of cytoplasmic extracts of mouse L cells. Two distinct groups of xyloA-substituted analogs could be readily disc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81358-3
更新日期:1987-02-23 00:00:00
abstract::The Ca(2+)-calmodulin dependent protein phosphatase, calcineurin, is thought to mediate the action of the two immunosuppressants, cyclosporin A (CsA) and FK506. Calcineurin from all species consists of a catalytic A subunit and a regulatory peptide B, which plays an essential role in catalysis. The enzymatic function ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01365-8
更新日期:1995-01-03 00:00:00