Abstract:
:The off rate from the first calcium-binding site of the C2A domain of synaptotagmin I, a putative calcium receptor in neurotransmitter release, has been determined by 15N-nuclear magnetic resonance relaxation dispersion measurements. The exchange rate was obtained by fitting the dependence of the transverse relaxation rates on the interval between 180 degrees pulses in relaxation-compensated CPMG experiments at 3.2 microM calcium concentration. The measured k(ex) is 2.0x10(3) x s(-1). The calcium on rate of 3.5+/-1x10(7) x s(-1), determined from the measured off rate and the dissociation constant (5.3x10(-5) M), is close to the diffusion limit. These results are consistent with the proposed role of synaptotagmin I as a calcium sensor in release, but suggest that additional factors may help to accelerate the diffusion of Ca2+ to the sensor.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Millet O,Bernadó P,Garcia J,Rizo J,Pons Mdoi
10.1016/s0014-5793(02)02508-5keywords:
subject
Has Abstractpub_date
2002-04-10 00:00:00pages
93-6issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302025085journal_volume
516pub_type
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