Identification of a novel prokaryotic HEAT-repeats-containing protein which interacts with a cyanobacterial IscA homolog.

Abstract:

:IscA homologs are known to be involved in iron-sulfur cluster formation in various organisms. Recombinant proteins of two IscA homologs from the cyanobacterium Synechocystis PCC 6803, designated SLR1417 and SLR1565, were purified. The absorption spectrum of purified SLR1565 was typical for [2Fe-2S] cluster-containing proteins, whereas that of SLR1417 predominantly showed the presence of the iron ion alone. In the cyanobacterial cell extracts, only SLR1565 was found to form a complex with a novel prokaryotic HEAT-repeats-containing protein, SLR1098. Thus, the two cyanobacterial IscA protein homologs exist in distinct molecular states, suggesting different cellular roles for these proteins.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Morimoto K,Nishio K,Nakai M

doi

10.1016/s0014-5793(02)02736-9

keywords:

subject

Has Abstract

pub_date

2002-05-22 00:00:00

pages

123-7

issue

1-3

eissn

0014-5793

issn

1873-3468

pii

S0014579302027369

journal_volume

519

pub_type

杂志文章
  • Spatial precision of a catalytic carboxylate of F1-ATPase beta subunit probed by introducing different carboxylate-containing side chains.

    abstract::Combining mutation and chemical modification, we have introduced Asp, Gln, Cys, S-carboxymethylcysteine (Cax) and S-carbamoylmethylcysteine (Cam) into the positions of Glu190 and Glu201 of the beta subunit of F1-ATPase from the thermophilic Bacillus PS3. The steady-state ATPase activities of alpha 3 beta 3 gamma compl...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)00588-5

    authors: Amano T,Tozawa K,Yoshida M,Murakami H

    更新日期:1994-07-04 00:00:00

  • Study of hepatic binding protein activity in jejuno-ileal by-passed rat hepatocytes.

    abstract::The kinetic constants of internalization of asialoorosomucoid were determined for normal and jejuno-ileal by-passed rat hepatocytes. In by-passed rats the maximum velocity of asialoorosomucoid internalization is decreased 3-fold, without any modification of apparant constant of internalization. Moreover, the rate cons...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(85)80783-3

    authors: Serbource-Goguel N,Dodeur M,Borel B,Feger J,Agneray J,Durand G

    更新日期:1985-04-22 00:00:00

  • Effects of 'hydrophobic mismatch' on the location of transmembrane helices in the ER membrane.

    abstract::We have studied the effects of 'hydrophobic mismatch' between a poly-Leu transmembrane helix (TMH) and the ER membrane using a glycosylation mapping approach. The simplest interpretation of our results is that the lumenal end of the TMH is located deeper in the membrane for both short (negative mismatch) and long (pos...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)02415-2

    authors: Monné M,von Heijne G

    更新日期:2001-05-11 00:00:00

  • Halving the selenophosphate synthetase gene dose confers hypersensitivity to oxidative stress in Drosophila melanogaster.

    abstract::Several lines of evidence indicate that selenoproteins mainly act as cellular antioxidants. Here, we test this idea comparing the sensitivity to oxidative stress (paraquat and hydrogen peroxide) between wild type and heterozygous flies for the selenophosphate synthetase selD(ptuf) mutation. Whereas under normal labora...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)03790-0

    authors: Morey M,Serras F,Corominas M

    更新日期:2003-01-16 00:00:00

  • Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport.

    abstract::The mechanisms involved in bidirectional transport along microtubules remain largely unknown. We explored the collective action of kinesin-2 and dynein motors during transport of melanosomes in Xenopus laevis melanophores. These motors are attached to organelles through accessory proteins establishing a complex molecu...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2015.07.038

    authors: De Rossi MC,De Rossi ME,Sued M,Rodríguez D,Bruno L,Levi V

    更新日期:2015-09-14 00:00:00

  • The SAAS granin exhibits structural and functional homology to 7B2 and contains a highly potent hexapeptide inhibitor of PC1.

    abstract::Prohormone convertases (PCs) 1 and 2 are thought to mediate the proteolytic cleavage of many peptide precursors. Endogenous inhibitors of both PC1 and PC2 have now been identified; the 7B2 protein is a nanomolar inhibitor of PC2, while the novel protein proSAAS was recently reported to be a micromolar inhibitor of PC1...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(00)01511-8

    authors: Cameron A,Fortenberry Y,Lindberg I

    更新日期:2000-05-12 00:00:00

  • Chloride channels of intracellular membranes.

    abstract::Proteins implicated as intracellular chloride channels include the intracellular ClC proteins, the bestrophins, the cystic fibrosis transmembrane conductance regulator, the CLICs, and the recently described Golgi pH regulator. This paper examines current hypotheses regarding roles of intracellular chloride channels an...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2010.01.037

    authors: Edwards JC,Kahl CR

    更新日期:2010-05-17 00:00:00

  • EPR spectroscopic characterization of an 'iron only' nitrogenase. S = 3/2 spectrum of component 1 isolated from Rhodobacter capsulatus.

    abstract::The alternative nitrogenase of Rhodobacter capsulatus, isolated from a nifHDK deletion mutant, has been purified to near homogeneity and identified as an 'iron only' nitrogenase. The dithionite-reduced component 1 ('FeFe protein') of this enzyme showed an EPR spectrum consisting of two components: a minor S = 1/2 sign...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80472-s

    authors: Müller A,Schneider K,Knüttel K,Hagen WR

    更新日期:1992-05-25 00:00:00

  • Evidence for the presence of two distinct sites of sucrose hydrolysis and glucosyl transfer activities on 1,3-alpha-D-glucan synthase of Streptococcus mutans.

    abstract::1,3-alpha-D-Glucan synthase of Streptococcus mutans catalyzes both the hydrolysis of sucrose to glucose and fructose, and the glucosyl transfer to glucosyl polymers to yield water-insoluble glucan. The enzyme catalyzes only sucrose hydrolysis, however, in the absence of 1,6-alpha-D-glucan as an acceptor. In the presen...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)80158-9

    authors: Yamashita Y,Hanada N,Itoh-Andoh M,Takehara T

    更新日期:1989-01-30 00:00:00

  • TLC immunostaining characterization of Clostridium botulinum type A neurotoxin binding to gangliosides and free fatty acids.

    abstract::The receptor structure of Clostridium botulinum neurotoxin type A was analysed by TLC immunostaining. GQ1b was found to be the most potent receptor, and the neurotoxin also bound to GT1b and GD1a, but not to GM3, GM2, GM1, GD3, GD1b and GT1a. Optimum binding of neurotoxin to the ganglioside appeared in 0.01 M phosphat...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(86)80614-7

    authors: Takamizawa K,Iwamori M,Kozaki S,Sakaguchi G,Tanaka R,Takayama H,Nagai Y

    更新日期:1986-06-09 00:00:00

  • AP-site cleavage activity of tyrosyl-DNA phosphodiesterase 1.

    abstract::APE-independent base excision repair (BER) pathway plays an important role in the regulation of DNA repair mechanisms. In this study it has been found that recently discovered tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the AP site cleavage reaction to generate breaks with the 3'- and 5'-phosphate termini. The re...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2011.01.032

    authors: Lebedeva NA,Rechkunova NI,Lavrik OI

    更新日期:2011-02-18 00:00:00

  • Sargaquinoic acid and sargahydroquinoic acid from Sargassum yezoense stimulate adipocyte differentiation through PPARalpha/gamma activation in 3T3-L1 cells.

    abstract::We screened active compounds from natural marine products able to increase PPARalpha/gamma transcriptional activity. Sargaquinoic acid (SQA) and sargahydroquinoic acid (SHQA) from Sargassum yezoense were identified as novel PPARalpha/gamma dual agonists. The binding affinity of SQA with PPARgamma was higher than that ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2008.09.011

    authors: Kim SN,Choi HY,Lee W,Park GM,Shin WS,Kim YK

    更新日期:2008-10-15 00:00:00

  • Crystallization and preliminary X-ray crystallographic study of interleukin-8.

    abstract::Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(90)80876-k

    authors: Auer M,Kallen J,Schleischitz S,Walkinshaw MD,Wasserbauer E,Ehn G,Lindley IJ

    更新日期:1990-06-04 00:00:00

  • Na(+)-dependent high affinity uptake of L-glutamate in primary cultures of human fibroblasts isolated from three different types of tissue.

    abstract::Cultured human fibroblasts isolated from embryonic muscle, skin and peripheral nerve tissues were found to accumulate [3H]L-glutamate by a Na(+)-dependent uptake process strongly inhibited by several glutamate/aspartate analogues including D- and L-aspartate, D- and L-threo-3-hydroxyaspartate and L-trans-pyrrolidine-2...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)80382-x

    authors: Balcar VJ,Shen J,Bao S,King NJ

    更新日期:1994-02-14 00:00:00

  • ERp57 interacts with conserved cysteine residues in the MHC class I peptide-binding groove.

    abstract::The oxidoreductase ERp57 is a component of the major histocompatibility complex (MHC) class I peptide-loading complex. ERp57 can interact directly with MHC class I molecules, however, little is known about which of the cysteine residues within the MHC class I molecule are relevant to this interaction. MHC class I mole...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.04.034

    authors: Antoniou AN,Santos SG,Campbell EC,Lynch S,Arosa FA,Powis SJ

    更新日期:2007-05-15 00:00:00

  • Insulin potentiates the transactivation potency of the glucocorticoid receptor.

    abstract::A single copy of a glucocorticoid-responsive element (GRE) is sufficient in mediating the combinatorial response of a promoter to both glucocorticoids and insulin in HepG2 cells. This requires the presence of active glucocorticoid receptor (GR) since the response is significantly inhibited by the anti-glucocorticoid R...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(96)00115-9

    authors: Georgakopoulos A,Tsawdaroglou N

    更新日期:1996-03-04 00:00:00

  • A mutational analysis of active site residues in trans-3-chloroacrylic acid dehalogenase.

    abstract::trans-3-Chloroacrylic acid dehalogenase (CaaD) catalyzes the hydrolytic dehalogenation of trans-3-haloacrylates to yield malonate semialdehyde by a mechanism utilizing βPro-1, αArg-8, αArg-11, and αGlu-52. These residues are implicated in a promiscuous hydratase activity where 2-oxo-3-pentynoate is processed to acetop...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2013.07.006

    authors: Poelarends GJ,Serrano H,Huddleston JP,Johnson WH Jr,Whitman CP

    更新日期:2013-09-02 00:00:00

  • The induction of adipose conversion in 3T3-L1 cells is associated with early phosphorylation of a 60-kDa nuclear protein.

    abstract::The induction of adipose conversion in 3T3-L1 cells by bezafibrate has been shown to be enhanced by dibutyryl-cAMP. We here report that the induction of adipose conversion in 3T3-L1 cells, by either bezafibrate and dibutyryl cAMP, or isobutylmethyxanthine alone, is associated with a very early phosphorylation of a 60-...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80725-i

    authors: Brandes R,Arad R,Bar-Tana J

    更新日期:1991-07-08 00:00:00

  • 'Neuron-specific' protein gene product 9.5 (PGP 9.5) is also expressed in glioma cell lines and its expression depends on cellular growth state.

    abstract::Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)81242-z

    authors: Giambanco I,Bianchi R,Ceccarelli P,Pula G,Sorci G,Antonioli S,Bocchini V,Donato R

    更新日期:1991-09-23 00:00:00

  • Anion channels and transporters in plant cell membranes.

    abstract::Anion channels/transporters appear as key players in signaling pathways leading to the adaptation of plant cells to abiotic and biotic environmental stresses, in the control of metabolism and in the maintenance of electrochemical gradients. Focusing on the most recent advances, this review aims at providing a descript...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2007.04.003

    authors: de Angeli A,Thomine S,Frachisse JM,Ephritikhine G,Gambale F,Barbier-Brygoo H

    更新日期:2007-05-25 00:00:00

  • Identification of a third yeast mitochondrial Tom protein with tetratrico peptide repeats.

    abstract::The mitochondrial outer membrane contains a protein complex with at least eight subunits responsible for recognition and translocation of preproteins synthesized in the cytosol. Two subunits, the receptors Tom20 and Tom70, contain tetratrico peptide repeats that are thought to be involved in protein-protein interactio...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(96)00156-1

    authors: Bömer U,Pfanner N,Dietmeier K

    更新日期:1996-03-11 00:00:00

  • Comparative EPR studies on the nitrite reductases from Escherichia coli and Wolinella succinogenes.

    abstract::Hexaheme nitrite reductases purified to homogeneity from Escherichia coli K-12 and Wolinella succinogenes were studied by low-temperature EPR spectroscopy. In their isolated states, the two enzymes revealed nearly identical EPR spectra when measured at 12 K. Both high-spin and low-spin ferric heme EPR resonances with ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)81051-7

    authors: Liu MC,Liu MY,Payne WJ,Peck HD Jr,Le Gall J,DerVartanian DV

    更新日期:1987-06-29 00:00:00

  • Yeast as a model system for studying lipid homeostasis and function.

    abstract::Lipids are essential eukaryotic cellular constituents. Lipid metabolism has a strong impact on cell physiology, and despite good progress in this area, many important basic questions remain unanswered concerning the functional diversity of lipid species and on the mechanisms that cells employ to sense and adjust their...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2012.07.033

    authors: Santos AX,Riezman H

    更新日期:2012-08-31 00:00:00

  • Rapid purification of malate synthase from cotyledons of Brassica napus L.

    abstract::A rapid and efficient method for the purification of malate synthase, an enzyme uniquely confined to glyoxysomes, from cotyledons of Brassica napus L. has been developed. The two step purification procedure is based on the consequent utilization of the tendency of malate synthase to form high molecular weight aggregat...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)01114-t

    authors: Hoppe A,Theimer RR

    更新日期:1995-10-30 00:00:00

  • Bisphosphorylation of cardiac troponin I modulates the Ca(2+)-dependent binding of myosin subfragment S1 to reconstituted thin filaments.

    abstract::We have reconstituted thin filaments comprising pyrene-labelled actin (pyr-actin), tropomyosin (Tm) and cardiac troponin (cTn). cTn was isolated in two defined phosphorylation states; completely dephosphorylated on all subunits and with only the cTnI subunit bisphosphorylated. The thin filament was saturated with cTn ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(96)00274-8

    authors: Reiffert SU,Jaquet K,Heilmeyer LM Jr,Ritchie MD,Geeves MA

    更新日期:1996-04-08 00:00:00

  • Adsorption of human lysozyme onto hydroxyapatite. Identification of its adsorbing site using site-directed mutagenesis.

    abstract::To elucidate hydroxyapatite-protein interaction, mutant human lysozymes in which the surface charge was modified by site-directed mutagenesis were used. Five mutant human lysozymes (K1A, K13A, K33A, R10A, R14A) were expressed in yeast. The chromatographic behavior of these lysozymes was studied with a HPLC hydroxyapat...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(97)01621-9

    authors: Aizawa T,Koganesawa N,Kamakura A,Masaki K,Matsuura A,Nagadome H,Terada Y,Kawano K,Nitta K

    更新日期:1998-01-30 00:00:00

  • Fluorescence digital image analysis of the inositol trisphosphate-mediated calcium transient in single permeabilized parietal cells.

    abstract::The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration [( Ca2+]i) increased in some restricted cytoplasmic r...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(88)80696-3

    authors: Tsunoda Y,Yodozawa S,Tashiro Y

    更新日期:1988-04-11 00:00:00

  • Channel-like NH3 flux by ammonium transporter AtAMT2.

    abstract::Prokaryotes, plants and animals control ammonium fluxes by the regulated expression of ammonium transporters (AMTs) and/or the related Rhesus (Rh) proteins. Plant AMTs were previously reported to mediate electrogenic transport. Functional analysis of AtAMT2 from Arabidopsis in yeast and oocytes suggests that NH(4)(+) ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2009.07.039

    authors: Neuhäuser B,Dynowski M,Ludewig U

    更新日期:2009-09-03 00:00:00

  • Coupled rotation within single F0F1 enzyme complexes during ATP synthesis or hydrolysis.

    abstract::F0F1 ATP synthases are the smallest rotary motors in nature and work as ATP factories in bacteria, plants and animals. Here we report on the first observation of intersubunit rotation in fully coupled single F0F1 molecules during ATP synthesis or hydrolysis. We investigate the Na+-translocating ATP synthase of Propion...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)03097-1

    authors: Kaim G,Prummer M,Sick B,Zumofen G,Renn A,Wild UP,Dimroth P

    更新日期:2002-08-14 00:00:00

  • Effect of starvation and insulin in vivo on the activity of the pyruvate dehydrogenase complex in rat skeletal muscles.

    abstract::The in vivo responses of pyruvate dehydrogenase (PDH) complex to starvation and insulin was assessed in heart, diaphragm and red quadriceps muscle. PDH complex activity was decreased by starvation (3.4-10.2-fold), the magnitude of change depending on muscle type. Insulin increased PDH activity in all muscle types. In ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)80777-x

    authors: Denyer GS,Lam D,Cooney GJ,Caterson ID

    更新日期:1989-07-03 00:00:00