Phosphorylation of smooth muscle myosin light chain by five different kinases.

abstract：:A rapid and efficient method for the purification of malate synthase, an enzyme uniquely confined to glyoxysomes, from cotyledons of Brassica napus L. has been developed. The two step purification procedure is based on the consequent utilization of the tendency of malate synthase to form high molecular weight aggregat...

journal_title：FEBS letters

authors： Hoppe A,Theimer RR

更新日期：1995-10-30 00:00:00

abstract：:Atrial natriuretic factor (ANF) and C-type natriuretic peptide (CNP)-activated guanylate cyclases are single-chain transmembrane-spanning proteins, containing both ligand binding and catalytic activities. In both proteins, ligand binding to the extracellular receptor domain activates the cytosolic catalytic domain, ge...

journal_title：FEBS letters

authors： Duda T,Goraczniak RM,Sharma RK

更新日期：1993-12-13 00:00:00

abstract：:We report for the first time the identification of 25 microRNAs from tissues originating from chicken embryo and adult chicken. Most of the cloned microRNAs are expressed in both adult chickens and chicken embryos. Fourteen were identified without any prior prediction. One microRNA, miR-757, is thought to be chicken-s...

journal_title：FEBS letters

authors： Xu H,Wang X,Du Z,Li N

更新日期：2006-06-26 00:00:00

abstract：:In plants exposed to high irradiances of visible light, the D1 protein in the reaction center of photosystem II is oxidatively damaged and rapidly degraded. Earlier work in our laboratory showed that the serine protease Deg2 performs the primary cleavage of photodamaged D1 protein in vitro. Here, we demonstrate that t...

journal_title：FEBS letters

authors： Huesgen PF,Schuhmann H,Adamska I

更新日期：2006-12-22 00:00:00

abstract：:A novel gene encoding a new H3 histone varian (H3L) has been identified in P. lividus sea urchin embryo. It encodes a H3 histone protein showing the S.//.A.IG amino acid motif typical of the replication independent H3.3 variants but in a mRNA showing the 3' terminal stem-loop nucleotide sequence that is typical of the...

journal_title：FEBS letters

authors： Fucci L,Forte A,Mancini P,Affaitati A,Branno M,Aniello F,Geraci G

更新日期：1997-04-21 00:00:00

abstract：:Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reac...

journal_title：FEBS letters

authors： Song K,Jung D,Jung Y,Lee SG,Lee I

更新日期：2000-09-08 00:00:00

abstract：:In order to identify physiological activators of proteinase-activated receptor-2 (PAR-2), a peptide chloromethane inhibitor (biotinyl-Ser-Lys-Gly-Arg-CH2Cl) based on the cleavage site for activation of PAR-2 was synthesised and tested with 12 trypsin-like serine proteinases. The second-order rate constant (ki/Ki) for ...

journal_title：FEBS letters

authors： Fox MT,Harriott P,Walker B,Stone SR

更新日期：1997-11-17 00:00:00

abstract：:Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiment...

journal_title：FEBS letters

authors： Eckes B,Mauch C,Hüppe G,Krieg T

更新日期：1993-03-01 00:00:00

abstract：:A cell-free protein expression system was established that provides protein samples of adequate concentration and purity for direct NMR analysis. The Escherichia coli peptidyl-prolyl cis-trans isomerase PpiB was expressed in this system with dual amino acid-selective isotope labeling to identify the NMR signal from th...

journal_title：FEBS letters

authors： Guignard L,Ozawa K,Pursglove SE,Otting G,Dixon NE

更新日期：2002-07-31 00:00:00

abstract：:The primary structure of 20 beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was determined after FPLC purification of a commercial preparation. Peptides obtained from different proteolytic cleavages were purified by reverse phase HPLC. The 255-residue structure deduced was found to be distantly homolog...

journal_title：FEBS letters

authors： Marekov L,Krook M,Jörnvall H

更新日期：1990-06-18 00:00:00

abstract：:The amino acid sequence of rat ribosomal protein L26 was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the NH2-terminal amino acid sequence of the protein. Rat L26 contains 145 amino acids and has a molecular mass of 17,266 Da. Hybridization of the cDNA to digests of nuclear DNA sug...

journal_title：FEBS letters

authors： Paz V,Olvera J,Chan YL,Wool IG

更新日期：1989-07-17 00:00:00

abstract：:Epidermal growth factor (EGF) dose-dependently enhanced the induction of tyrosine aminotransferase and tryptophan oxygenase by glucocorticoids in primary cultures of adult rat hepatocytes without itself having any effect on these enzymes in the absence of glucocorticoids. The amplifications were observed even with dex...

journal_title：FEBS letters

authors： Kido H,Fukusen N,Katunuma N

更新日期：1987-11-02 00:00:00

abstract：:A membrane-bound phospholipase C-like hydrolase present in lysates of bloodstream forms of Trypanosoma brucei rapidly converts the membrane form of the variant surface protein to the soluble form and 1,2-dimyristoylglycerol [(1985) M.A.J. Ferguson et al. J. Biol. Chem., 260, 4963-4968]. The hydrolase is inhibited by p...

journal_title：FEBS letters

authors： Bülow R,Overath P

更新日期：1985-07-22 00:00:00

abstract：:The TSH-induced cyclic AMP response was studied using a 3-year-old ovine thyroid cell line TSH-independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave ...

journal_title：FEBS letters

authors： Fayet G,Aouani A,Hovsépian S

更新日期：1986-01-06 00:00:00

abstract：:Male germ cell Rac GTPase-activating protein (MgcRacGAP) is a core regulator of cytokinesis. Furthermore, it appears to be involved in human oncogenesis through cytokinesis-independent mechanisms and has been reported to be essential for nuclear translocation of signal transducer and activator of transcription (STAT) ...

journal_title：FEBS letters

authors： van Adrichem AJ,Wennerberg K

更新日期：2015-12-21 00:00:00

abstract：:Association of protein inclusions or aggregates within brain tissues of patients with neurodegenerative disorders has been widely reported. These inclusions are commonly characterised both by the presence of ubiquitylated proteins and the sequestration of components of the ubiquitin-proteasome system (UPS). Such obser...

journal_title：FEBS letters

authors： Ardley HC,Hung CC,Robinson PA

更新日期：2005-01-31 00:00:00

abstract：:Spectral variants of the green fluorescent protein (GFP) have been extensively used as reporters to image molecular interactions in living cells by fluorescence resonance energy transfer (FRET). However, those GFP variants which are the most efficient donor acceptor pairs for FRET measurements show a high degree of sp...

journal_title：FEBS letters

authors： Zimmermann T,Rietdorf J,Girod A,Georget V,Pepperkok R

更新日期：2002-11-06 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1alpha), a highly inducible transcriptional coactivator regulating energy homeostasis, is down-regulated in hepatoma tissues. To dissect its role in hepato-tumorigenesis, Ingenuity Pathway Analysis was applied to construct pathways affected by P...

journal_title：FEBS letters

authors： Lee HJ,Su Y,Lui WY,Chau GY,Yin PH,Lee HC,Chi CW

更新日期：2008-03-05 00:00:00

abstract：:Organ failure induced by endotoxic shock has recently been associated with affected mitochondrial function. In this study, effects of in vivo lipopolysaccharide-challenge on protein patterns of rat liver mitochondria in treated animals versus controls were studied by two-dimensional electrophoresis (differential image...

journal_title：FEBS letters

authors： Miller I,Gemeiner M,Gesslbauer B,Kungl A,Piskernik C,Haindl S,Nürnberger S,Bahrami S,Redl H,Kozlov AV

更新日期：2006-02-20 00:00:00

abstract：:Ghrelin is a novel gastrointestinal peptide that stimulates growth hormone secretion, food intake, and body weight gain. Increased ghrelin secretion has been reported in such negative energy states as starvation and low body weight. We investigated the dynamics of ghrelin in rats with streptozotocin-induced diabetes, ...

journal_title：FEBS letters

authors： Masaoka T,Suzuki H,Hosoda H,Ota T,Minegishi Y,Nagata H,Kangawa K,Ishii H

更新日期：2003-04-24 00:00:00

abstract：:The interaction of water oxidising photosystem II preparations with the aqueous environment has been investigated using electron spin echo envelope modulation spectroscopy in the presence of 2H(2)O. The spectra show interaction of 2H of 2H(2)O with the preparation in the S(2) state. The component interacting with wate...

journal_title：FEBS letters

authors： Evans MC,Rich AM,Nugent JH

更新日期：2000-07-14 00:00:00

abstract：:Cytochrome oxidase o has been isolated from the obligately aerobic, methylotrophic bacterium Methylophilus methylotrophus in the form of a cytochrome cL-o complex. The latter is comprised of cytochrome cL (Mr 21 000) and cytochrome o (Mr 29 000) in a 1-2:1 ratio, possibly in association with one or more minor polypept...

journal_title：FEBS letters

authors： Carver MA,Jones CW

更新日期：1983-05-08 00:00:00

abstract：:Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol ...

journal_title：FEBS letters

authors： Odom OW,Stöffler G,Hardesty B

更新日期：1984-07-23 00:00:00

abstract：:The insulin-like growth factor binding proteins (IGFBPs) are responsible for regulation of the effects and the bioavailability of the insulin-like growth factors (IGFs). We screened for circulating fragments of human IGFBP-5 in human hemofiltrate. Identification of IGFBP-5 peptides in the fractions of our peptide bank...

journal_title：FEBS letters

authors： Ständker L,Wobst P,Mark S,Forssmann WG

更新日期：1998-12-18 00:00:00

abstract：:A double-blinded, placebo-controlled cross-over trial was carried out with 27 hypercholesterolemic men with coronary heart disease. During the 6-week treatment period lovastatin (60 mg/day) decreased fasting serum LDL cholesterol by 45%, LDL phosphorus by 38% and apoB by 33%. Ubiquinol content diminished by 13% as mea...

journal_title：FEBS letters

authors： Palomäki A,Malminiemi K,Metsä-Ketelä T

更新日期：1997-06-30 00:00:00

abstract：:Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 microM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate ef...

journal_title：FEBS letters

authors： Klimov VV,Allakhverdiev SI,Feyziev YaM,Baranov SV

更新日期：1995-04-24 00:00:00

abstract：:Lipids are essential eukaryotic cellular constituents. Lipid metabolism has a strong impact on cell physiology, and despite good progress in this area, many important basic questions remain unanswered concerning the functional diversity of lipid species and on the mechanisms that cells employ to sense and adjust their...

journal_title：FEBS letters

authors： Santos AX,Riezman H

更新日期：2012-08-31 00:00:00

abstract：:Protein kinase B (PKB, also named as Akt or RAC-protein kinase), that is activated by cellular stress such as heat shock and hyperosmotic treatment, was revealed to be activated by oxidative stress and by chemical stressors of CdCl2 and NaAsO2 by measuring the activity of the enzyme immunoprecipitated from the transfe...

journal_title：FEBS letters

authors： Konishi H,Matsuzaki H,Tanaka M,Takemura Y,Kuroda S,Ono Y,Kikkawa U

更新日期：1997-06-30 00:00:00

abstract：:Induction of differentiation in HL-60 and U-937 leukemic cell lines, resulted in 1.5-10-fold increase in 45Ca2+ uptake. The increased 45Ca2+ uptake in the differentiating cells was inhibited by verapamil, cromolyn and amiloride. Elevation in Ca2+ uptake in differentiating cells was also demonstrated using the fluoresc...

journal_title：FEBS letters

authors： Rephaeli A,Aviram A,Englender T,Shaklai M

更新日期：1988-08-29 00:00:00

abstract：:Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system, trpl expressed in Sf9 cells showed spo...

journal_title：FEBS letters

authors： Harteneck C,Obukhov AG,Zobel A,Kalkbrenner F,Schultz G

更新日期：1995-01-30 00:00:00