Abstract:
:Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reactive clones were found to encode the dimerization domain of TRF2, a mammalian telomeric protein that binds to duplex TTAGGG repeats at chromosome ends. This interaction was confirmed using bacterial fusion proteins and co-immunoprecipitations from eukaryotic cells overexpressing TRF2. The transfected TFR2 colocalized with Ku70.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Song K,Jung D,Jung Y,Lee SG,Lee Idoi
10.1016/s0014-5793(00)01958-xkeywords:
subject
Has Abstractpub_date
2000-09-08 00:00:00pages
81-5issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01958-Xjournal_volume
481pub_type
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