Abstract:
:In the filamentous fungus Neurospora, the production of dsRNA can elicit a dsRNA-induced transcriptional response similar to the interferon response in vertebrates. However, how fungi sense the expression of dsRNA and activate gene expression is unknown. In this study, we established a dsRNA response reporter system in Neurospora crassa. Using the dsRNA-activated RNA-dependent RNA polymerase gene rrp-3 promoter, we created an expression construct (pRRP-3::Myc-Al-1) and introduced it into al-1(KO) mutant. The test dsRNA efficiently induced pRRP-3::Myc-Al-1 expression in the al-1(KO) mutant, resulting in conidia color switching from white to yellow. These results confirm that the dsRNA response is regulated at the transcriptional level and this reporter system can be used for future studies in dsRNA response in filamentous fungi.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Li X,Shen Y,Sun J,Wang B,He Qdoi
10.1016/j.febslet.2011.02.024subject
Has Abstractpub_date
2011-03-23 00:00:00pages
906-12issue
6eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00128-1journal_volume
585pub_type
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