The vitamin D3 analogue, calcipotriol, induces sphingomyelin hydrolysis in human keratinocytes.

abstract：:Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca(2+)-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC8) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These r...

journal_title：FEBS letters

authors： Sanchez-Bueno A,Cobbold PH

更新日期：1992-11-02 00:00:00

abstract：:To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated wi...

journal_title：FEBS letters

authors： Pokholok DK,Gudima SO,Yesipov DS,Dobrynin VN,Rechinsky VO,Kochetkov SN

更新日期：1993-07-05 00:00:00

abstract：:The selectively red excited emission spectrum, at room temperature, of the in vitro reconstituted Lhca4, has a pronounced non-equilibrium distribution, leading to enhanced emission from the directly excited low-energy pigments. Two different emitting forms (or states), with maximal emission at 713 and 735nm (F713 and ...

journal_title：FEBS letters

authors： Zucchelli G,Morosinotto T,Garlaschi FM,Bassi R,Jennings RC

更新日期：2005-04-11 00:00:00

abstract：:Cytotoxic effects were observed following 4 h incubation of human leukaemic cells with the iron chelator 1-hydroxypyridine-2-thione (omadine). Its cytotoxic activity was comparable to that of the cytotoxic drug doxorubicin. At the same concentration two other effective iron chelators, desferrioxamine and 1,2-dimethyl-...

journal_title：FEBS letters

authors： Kontoghiorghes GJ,Piga A,Hoffbrand AV

更新日期：1986-08-18 00:00:00

abstract：:Wilms tumor (WT) is a genetically heterogeneous childhood kidney tumor. Several genetic mutations have been identified in WT patients, including inactivation of WTX, somatic stabilizing CTNNB1, and p53 mutations. However, the molecular mechanisms in tumorigenesis remain largely unexplored. Stat3 is a transcription fac...

journal_title：FEBS letters

authors： Cao X,Liu D,Yan X,Zhang Y,Yuan L,Zhang T,Fu M,Zhou Y,Wang J

更新日期：2013-03-18 00:00:00

abstract：:The phosphatidylinositol 3-kinase/Akt pathway is responsible for key aspects of tumor progression, and is frequently hyperactivated in cancer. We have recently identified palladin, an actin-bundling protein that functions to control the actin cytoskeleton, as an Akt1-specific substrate that inhibits breast cancer cell...

journal_title：FEBS letters

authors： Chin YR,Toker A

更新日期：2010-12-01 00:00:00

abstract：:Intracellular accumulation of anthracycline derivatives was measured in a human embryonic kidney cell line (HEK) and a resistant subline (HEK/multidrug resistance protein (MRP1)) overexpressing MRP1 at the plasma membrane surface. Two compounds (daunorubicin and doxorubicin) were rejected outside the multidrug-resista...

journal_title：FEBS letters

authors： Manciu L,Chang X,Riordan JR,Buyse F,Ruysschaert JM

更新日期：2001-03-23 00:00:00

abstract：:Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in chloroform-methanol (1:1 v/v), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of alpha-helices, reverse turns and beta-sheets are similar to t...

journal_title：FEBS letters

authors： Torres J,Padrós E

更新日期：1993-02-22 00:00:00

abstract：:Oxygen is an essential element in the survival of complex organisms, however the level of oxygen, low or high, can be a source of stress depending on the biological context. Low levels of oxygen in tissues (hypoxia) can be the consequence of a number of pathophysiological conditions including ischemic disorders and ca...

journal_title：FEBS letters

authors： Brahimi-Horn MC,Pouysségur J

更新日期：2007-07-31 00:00:00

abstract：:Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...

journal_title：FEBS letters

authors： Hübner D,Drakenberg T,Forsén S,Fischer G

更新日期：1991-06-17 00:00:00

abstract：:Undifferentiated rat pheochromocytoma PC12 cells were voltage clamped using the whole cell technique. After blockade of outward currents, calcium currents were elicited from -40 and -100 mV. A subpopulation of cells displayed only one current component activated at -10 mV and slowly decaying. In other cells this curre...

journal_title：FEBS letters

authors： Janigro D,Maccaferri G,Meldolesi J

更新日期：1989-09-25 00:00:00

abstract：:The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP2) and 'caged' guanosine ...

journal_title：FEBS letters

authors： Berven LA,Barritt GJ

更新日期：1994-06-13 00:00:00

abstract：:Bovine leukaemia virus (BLV) is the aetiological agent of Leukosis enzootica bovis [Viral Oncology (1980), G. Klein (Ed.) Raven Press, New York, pp. 231-238], a widely spread disease in cattle. BLV is reported as the animal model of human T-cell leukaemia virus (HLTV) which is the causative agent of adult T-cell leuka...

journal_title：FEBS letters

authors： Précigoux G,Geoffre S,Léonard R,Llido S,Dautant A,d'Estaintot BL,Picard P,Ménard A,Guillemain B,Hospital M

更新日期：1993-07-12 00:00:00

abstract：:The amino acid sequences of two ribonucleases from a callus cell culture of Panax ginseng were determined. The two sequences differ at 26% of the amino acid positions. Homology was found with a large family of intracellular pathogenesis-related proteins, food allergens and tree pollen allergens from both dicotyledonou...

journal_title：FEBS letters

authors： Moiseyev GP,Fedoreyeva LI,Zhuravlev YN,Yasnetskaya E,Jekel PA,Beintema JJ

更新日期：1997-04-28 00:00:00

abstract：:Phosphate triester derivatives of the anti-neoplastic alkyl lyso phospholipid (ALP) have been prepared as novel potential therapeutic agents. In particular, symmetrical phosphate triesters have been prepared, using phosphorochloridate chemistry. The compounds have been fully characterised by a range of techniques, and...

journal_title：FEBS letters

authors： McGuigan C,Wang Y,Riley PA

更新日期：1995-09-25 00:00:00

abstract：:Under destabilising conditions both heat and cold denaturation of yeast phosphoglycerate kinase (PGK) can be observed. According to previous interpretation of experimental data and theoretical calculations, the C-terminal domain should be more stable than the N-terminal domain at all temperatures. We report on thermal...

journal_title：FEBS letters

authors： Gast K,Damaschun G,Desmadril M,Minard P,Müller-Frohne M,Pfeil W,Zirwer D

更新日期：1995-01-30 00:00:00

abstract：:The effect of increasing low density lipoprotein (LDL) concentrations on the synthesis of basement membrane components was investigated in proliferating porcine aortic endothelial cells (PAEC) in culture. Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin were determined by enzyme immunoa...

journal_title：FEBS letters

authors： Olgemöller B,Schleicher ED,Schwaabe S,Guretzki HJ,Gerbitz KD

更新日期：1990-05-07 00:00:00

abstract：:As yet, the physiological significance of hydroxylation of anandamide and linoleoyl amides is unknown. Therefore, we investigated whether hydroxylation of ODNHEtOH and ODNH2 influences their binding abilities to the CB-1 receptor and whether it alters their reactivity towards a fatty acid amide hydrolase (FAAH) from r...

journal_title：FEBS letters

authors： van der Stelt M,Paoletti AM,Maccarrone M,Nieuwenhuizen WF,Bagetta G,Veldink GA,Finazzi Agrò A,Vliegenthart JF

更新日期：1997-10-06 00:00:00

abstract：:Studies were undertaken to further elucidate the mechanism(s) by which bradykinin-dependent phosphoinositide metabolism takes place in neuroblastoma X glioma hybrid NG108-15 cells [(1984) J. Biol. Chem. 259, 10201-10207] using [3H]inositol-labelled cells. Bradykinin produced net increases in the level of [3H]inositol ...

journal_title：FEBS letters

authors： Yano K,Higashida H,Hattori H,Nozawa Y

更新日期：1985-02-25 00:00:00

abstract：:Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement...

journal_title：FEBS letters

authors： Kawabe Ji,Ebina T,Toya Y,Oka N,Schwencke C,Duzic E,Ishikawa Y

更新日期：1996-04-22 00:00:00

abstract：:It is clear that G1-S phase control is exerted after the mouse embryo implants into the uterus 4.5 days after fertilization (E4.5); null mutants of genes that control cell cycle commitment such as max, rb (retinoblastoma), and dp1 are embryonic lethal after implantation with proliferation phenotypes. But, a number of ...

journal_title：FEBS letters

authors： Xie Y,Sun T,Wang QT,Wang Y,Wang F,Puscheck E,Rappolee DA

更新日期：2005-01-17 00:00:00

abstract：:Physicochemical properties of proteins are controlled mainly by post-translational modifications such as amino acid phosphorylation. Although molecular dynamics simulations have been shown to be a valuable tool for studying the effects of phosphorylation on protein structure and dynamics, most of the previous studies ...

journal_title：FEBS letters

authors： Kawade R,Kuroda D,Tsumoto K

更新日期：2020-03-01 00:00:00

abstract：:It is known that the DNA binding Runt domain of the AML1/Runx1 transcription factor coordinates Cl(-) ions. In this paper we have determined Cl(-) binding affinities of AML1 by (35)Cl nuclear magnetic resonance (NMR) linewidth analysis. The Runt domain binds Cl(-) with a dissociation constant (K(d,Cl)) of 34 mM. If CB...

journal_title：FEBS letters

authors： Wolf-Watz M,Bäckström S,Grundström T,Sauer U,Härd T

更新日期：2001-01-12 00:00:00

abstract：:A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

journal_title：FEBS letters

authors： Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

更新日期：1993-03-08 00:00:00

abstract：:Blood reprogramming, in which induced pluripotent stem cells (iPSCs) are derived from haematopoietic lineages, has rapidly advanced over the past decade. Since the first report using human blood, haematopoietic cell types from various sources, such as the peripheral bone marrow and cord blood, have been successfully r...

journal_title：FEBS letters

authors： Chen Y,Yi Y,Xu J,Chan WK,Loh YH

更新日期：2019-12-01 00:00:00

abstract：:A potent inhibitor of a dual-specificity protein phosphatase, VHR (vaccinia H1 related), was isolated during a screening of microbial metabolites. This inhibitor was identified as 4-isoavenaciolide (4-iA), and was determined to irreversibly inhibit VHR phosphatase activity with a 50% inhibitory concentration of 1.2 mi...

journal_title：FEBS letters

authors： Ueda K,Usui T,Nakayama H,Ueki M,Takio K,Ubukata M,Osada H

更新日期：2002-08-14 00:00:00

abstract：:The corpus of the scientific literature has reached such size that a lot of useful data, dispersed throughout millions different articles, are now hard to recover. For instance, many articles in the biological domain describe relationships between entities (gene, proteins, small molecules, etc.) yet this crucial infor...

journal_title：FEBS letters

authors： Ceol A,Chatr-Aryamontri A,Licata L,Cesareni G

更新日期：2008-04-09 00:00:00

abstract：:Equine platelet beta tropomyosin (247 residues), like rabbit skeletal muscle alpha tropomyosin (284 residues) has a repeating pattern of amino acid residues characteristic of a coiled-coil structure. When compared with the muscle protein, it is extended by 5 residues at the NH2-terminus and possesses two 21 residue de...

journal_title：FEBS letters

authors： Lewis WG,Cote GP,Mak AS,Smillie LB

更新日期：1983-06-13 00:00:00

abstract：:Transcriptional co-activators, co-repressors and chromatin remodeling machines are essential elements in the transcriptional programs directed by the master adipogenic transcription factor PPARgamma. Many of these components have orthologs in other organisms, where they play roles in development and pattern formation,...

journal_title：FEBS letters

authors： Denis GV,Nikolajczyk BS,Schnitzler GR

更新日期：2010-08-04 00:00:00

abstract：:Absence through mutation of two proteins involved in periplasmic disulphide bond formation, DsbA and DsbB, results in failure of anaerobically grown Escherichia coli to synthesise the holo forms of either its endogenous c-type cytochrome nitrite reductase or exogenous cytochrome c550 from Paracoccus denitrificans. The...

journal_title：FEBS letters

authors： Sambongi Y,Ferguson SJ

更新日期：1996-12-02 00:00:00