Abstract:
:It is known that the DNA binding Runt domain of the AML1/Runx1 transcription factor coordinates Cl(-) ions. In this paper we have determined Cl(-) binding affinities of AML1 by (35)Cl nuclear magnetic resonance (NMR) linewidth analysis. The Runt domain binds Cl(-) with a dissociation constant (K(d,Cl)) of 34 mM. If CBFbeta is added to form a 1:1 complex, the K(d,Cl) value increases to 56 mM. Homology modeling suggests that a high occupancy Cl(-) binding site overlaps with the DNA binding surface. NMR data show that DNA displaces this Cl(-) ion. Possible biological roles of Cl(-) binding are discussed based on these findings.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wolf-Watz M,Bäckström S,Grundström T,Sauer U,Härd Tdoi
10.1016/s0014-5793(00)02390-5keywords:
subject
Has Abstractpub_date
2001-01-12 00:00:00pages
81-4issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(00)02390-5journal_volume
488pub_type
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