Amiloride at pH 7.0 inhibits the Na(+)-driven flagellar motors of Vibrio alginolyticus but allows cell growth.

abstract：:The predicted Exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient Siberian permafrost sample. The protein bacteriorhodopsin from Exiguobacterium sibiricum (ESR) encoded by this gene was expressed in Escherichia coli membrane. ESR bound all-trans-retinal and displayed an absorbance maximum at 5...

journal_title：FEBS letters

authors： Petrovskaya LE,Lukashev EP,Chupin VV,Sychev SV,Lyukmanova EN,Kryukova EA,Ziganshin RH,Spirina EV,Rivkina EM,Khatypov RA,Erokhina LG,Gilichinsky DA,Shuvalov VA,Kirpichnikov MP

更新日期：2010-10-08 00:00:00

abstract：:Undifferentiated rat pheochromocytoma PC12 cells were voltage clamped using the whole cell technique. After blockade of outward currents, calcium currents were elicited from -40 and -100 mV. A subpopulation of cells displayed only one current component activated at -10 mV and slowly decaying. In other cells this curre...

journal_title：FEBS letters

authors： Janigro D,Maccaferri G,Meldolesi J

更新日期：1989-09-25 00:00:00

abstract：:A method was developed to study amino acids as possible precursors of PQQ biosynthesis. Cultures of Hyphomicrobium X, growing on [13C]methanol, were supplemented with unlabelled amino acids. Uptake and participation in metabolism were determined via gas chromatography/mass spectrometry of derivatized amino acids, obta...

journal_title：FEBS letters

authors： van Kleef MA,Duine JA

更新日期：1988-09-12 00:00:00

abstract：:Phospholipase D was first described in plant tissue but has recently been shown to occur in mammalian cells where it is activated by cell surface receptors. Its mode of activation by receptors in unclear. Biochemical studies suggest that it may occur downstream of other effector proteins and that small GTP-dependent r...

journal_title：FEBS letters

authors： Holbrook PG,Geetha V,Beaven MA,Munson PJ

更新日期：1999-04-09 00:00:00

abstract：:We studied the molecular binding specificity of two rabbit polyclonal sera generated against phenolic glycolipid antigens namely PheG1 B and PheG1 B-3 from Mycobacterium bovis BCG. PheG1 B is the well-known mycoside B (2-O-Me-alpha-L-Rhap 1----aglycone), while PheG1 B-3 is a recently found glycolipid (alpha-L-Rhap-(1-...

journal_title：FEBS letters

authors： Vercellone A,Riviere M,Fournié JJ,Puzo G

更新日期：1992-05-25 00:00:00

abstract：:As part of a search for selective, mechanism-based covalent inhibitors of human pancreatic α-amylase we describe the chemoenzymatic synthesis of the disaccharide analog α-glucosyl epi-cyclophellitol, demonstrate its stoichiometric reaction with human pancreatic α-amylase and evaluate the time dependence of its inhibit...

journal_title：FEBS letters

authors： Caner S,Zhang X,Jiang J,Chen HM,Nguyen NT,Overkleeft H,Brayer GD,Withers SG

更新日期：2016-04-01 00:00:00

abstract：:We previously described that NO. leads to destruction of ZnS clusters and release of Zn2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO. leads to an increase of cytoplasmic and nuclear free Zn2+. L929 cells, mouse splen...

journal_title：FEBS letters

authors： Berendji D,Kolb-Bachofen V,Meyer KL,Grapenthin O,Weber H,Wahn V,Kröncke KD

更新日期：1997-03-17 00:00:00

abstract：:The mitochondrial alternative oxidase is a diiron carboxylate quinol oxidase (Dox) found in plants and some fungi and protists, but not animals. The plastid terminal oxidase is distantly related to alternative oxidase and is most likely also a Dox protein. Database searches revealed that the alpha-proteobacterium Novo...

journal_title：FEBS letters

authors： Finnegan PM,Umbach AL,Wilce JA

更新日期：2003-12-18 00:00:00

abstract：:Conformational changes in the extradiscal regions of rhodopsin induced by illumination were investigated by modifying the visual pigment by mild treatment with cyanogen bromide prior to and after light exposure. Light induced an increased yield of cleavage of the Met bond 253-254 and a new cleavage at the Met bond 155...

journal_title：FEBS letters

authors： Pellicone C,Nullans G,Virmaux N

更新日期：1985-02-11 00:00:00

abstract：:The pET(scF11) plasmid was constructed comprising the gene of a single-chain antibody against human ferritin. This plasmid encodes the leader peptide pelB followed by the heavy chain variable V(H) domain, (Gly4Ser)3 linker peptide, and light chain variable V(L) domain. The correctly processed scF11 antibody was expres...

journal_title：FEBS letters

authors： Martsev SP,Kravchuk ZI,Chumanevich AA,Vlasov AP,Dubnovitsky AP,Bespalov IA,Arosio P,Deyev SM

更新日期：1998-12-28 00:00:00

abstract：:Using a cationic lipid bilayer, we show that DNA can be reliably adsorbed to the bilayer surface for atomic force microscopy (AFM) in aqueous buffers at high resolution. The measured width of the dsDNA is close to 2 nm, and a periodic modulation on dsDNA is reproducibly detected by the AFM. The measured period is 3.4 ...

journal_title：FEBS letters

authors： Mou J,Czajkowsky DM,Zhang Y,Shao Z

更新日期：1995-09-11 00:00:00

abstract：:Pancreatic islet amyloid is a characteristic feature of type 2 diabetes. The major protein component of islet amyloid is the polypeptide hormone known as islet amyloid polypeptide (IAPP, or amylin). IAPP is stored with insulin in the β-cell secretory granules and is released in response to the stimuli that lead to ins...

journal_title：FEBS letters

authors： Cao P,Marek P,Noor H,Patsalo V,Tu LH,Wang H,Abedini A,Raleigh DP

更新日期：2013-04-17 00:00:00

abstract：:The mutant hen egg white lysozymes Ile55Thr and Asp66His, corresponding to human amyloidogenic mutant lysozymes Ile56Thr and Asp67His, respectively, were secreted in Saccharomyces cerevisiae. The amyloidogenic mutants (I55T and D66H) of hen egg white lysozymes were remarkably less soluble than that of the wild-type pr...

journal_title：FEBS letters

authors： Song Y,Azakami H,Hamasu M,Kato A

更新日期：2001-02-23 00:00:00

abstract：:Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis ...

journal_title：FEBS letters

authors： Fischer M,Haase I,Simmeth E,Gerisch G,Müller-Taubenberger A

更新日期：2004-11-05 00:00:00

abstract：:A tetradecapeptide with a sequence identical to residues 26-39 of the cro protein from bacteriophage lambda has been synthesized. This peptide has no secondary structure in an aqueous buffer but adopts an alpha-helical conformation in the presence of 20% hexafluoroisopropanol. The fluorescence of the single tyrosyl re...

journal_title：FEBS letters

authors： Mayer R,Lancelot G,Hélène C

更新日期：1983-03-21 00:00:00

abstract：:We previously reported that IκBL prevents experimental autoimmune arthritis. The molecular mechanism, however, still remains unclear. In contrast to four splicing-isoforms of IκBL in human, two isoforms were identified in mouse. The major isoform IκBL-α(S) suppressed LPS-induced NF-κB activation and transcription of T...

journal_title：FEBS letters

authors： Chiba T,Miyashita K,Sugoh T,Warita T,Inoko H,Kimura M,Sato T

更新日期：2011-11-16 00:00:00

abstract：:Male germ cell Rac GTPase-activating protein (MgcRacGAP) is a core regulator of cytokinesis. Furthermore, it appears to be involved in human oncogenesis through cytokinesis-independent mechanisms and has been reported to be essential for nuclear translocation of signal transducer and activator of transcription (STAT) ...

journal_title：FEBS letters

authors： van Adrichem AJ,Wennerberg K

更新日期：2015-12-21 00:00:00

abstract：:The chaotropic character of several ions determines their partition on membrane interfaces with aqueous media as predicted by the Hofmeister series. However, specific characteristics of each individual ion determine its ability to cross the membrane and to influence Ca2+, H+ and electrical gradients produced by the sa...

journal_title：FEBS letters

authors： Yu X,Inesi G

更新日期：1993-08-16 00:00:00

abstract：:The octapeptide Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr (peptide T) and two structural analogs are potent agonists of human monocyte chemotaxis, evincing identical rank potency orders as was previously shown for their inhibition of human immunodeficiency virus (HIV) envelope binding and T cell infectivity. Chemotactic activit...

journal_title：FEBS letters

authors： Ruff MR,Martin BM,Ginns EI,Farrar WL,Pert CB

更新日期：1987-01-19 00:00:00

abstract：:In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8Å r...

journal_title：FEBS letters

authors： She Z,Gao ZQ,Liu Y,Wang WJ,Liu GF,Shtykova EV,Xu JH,Dong YH

更新日期：2012-07-30 00:00:00

abstract：:Apolipoprotein E (apoE) is predominantly synthesized by astrocytes in the brain. In this study, we investigated the role of apoE in astrocyte apoptosis. We demonstrated that apoE protects astrocytes from hypoxia-induced apoptosis in a dose-dependent manner. Glutamate release from astrocytic cultures is significantly l...

journal_title：FEBS letters

authors： Zhou S,Wu H,Zeng C,Xiong X,Tang S,Tang Z,Sun X

更新日期：2013-01-16 00:00:00

abstract：:The effect on cytosolic Ca2+ concentration ([Ca2+]i) of cAMP analogues and the adenylate cyclase-stimulating agents forskolin, isoproterenol and glucagon has been examined in an insulin-secreting beta-cell line (HIT T-15) using fura 2. All these manipulations of the cAMP messenger system promoted a rise in [Ca2+]i whi...

journal_title：FEBS letters

authors： Prentki M,Glennon MC,Geschwind JF,Matschinsky FM,Corkey BE

更新日期：1987-08-10 00:00:00

abstract：:The human gut microbiome refers to a highly diverse microbial ecosystem, which has a symbiotic relationship with the host. Molecular hydrogen (H2 ) and carbon dioxide (CO2 ) are generated by fermentative metabolism in anaerobic ecosystems. H2 generation and oxidation coupled to CO2 reduction to methane or acetate help...

journal_title：FEBS letters

authors： Hylemon PB,Harris SC,Ridlon JM

更新日期：2018-06-01 00:00:00

abstract：:Recently, reports have appeared which show structural variations in B-DNA and indicate deviations from a uniform helical structure. We report for the first time that these indications are also present in the B-form fibre diffraction patterns for the lithium salt of natural DNA. We have used an improved method of contr...

journal_title：FEBS letters

authors： Dutta S,Parrack PK,Sasisekharan V

更新日期：1984-10-15 00:00:00

abstract：:32P-Labelled tRNA was isolated from uninfected and phage phi 149-infected Vibrio cholerae cells. These tRNA preparations were then hybridised with DNA isolated from phage phi 149. Significant hybridisation was observed only with tRNA from phage phi 149-infected cells. This strongly suggests that infection of classical...

journal_title：FEBS letters

authors： Ghosh RK,Guhathakurta I

更新日期：1983-10-03 00:00:00

abstract：:Protein kinase CK2 is composed of two regulatory beta-subunits and two catalytic alpha- or alpha'-subunits. To analyse these subunits individually we generated antibodies against unique peptides derived from the alpha-, alpha'- and beta-subunit. Immunofluorescence studies with these antibodies revealed the presence of...

journal_title：FEBS letters

authors： Faust M,Schuster N,Montenarh M

更新日期：1999-11-26 00:00:00

abstract：:It is increasingly clear that microRNAs play a crucial role in tumorigenesis. Recently, emerging evidence suggested that miR-26a is aberrantly expressed in tumor tissues. In our study, frequent down-regulation of miR-26a was observed in 10 human bladder cancer tissues. Forced expression of miR-26a in the bladder cance...

journal_title：FEBS letters

authors： Lin Y,Chen H,Hu Z,Mao Y,Xu X,Zhu Y,Xu X,Wu J,Li S,Mao Q,Zheng X,Xie L

更新日期：2013-08-02 00:00:00

abstract：:The presence of additives during X-irradiation of malate synthase led to radioprotective effects against primary and post-irradiation inactivation. Pronounced effects were provided by typical scavengers, sulfhydryl reagents and specific ligands (substrates, products, analogues). The results show that scavenging and sp...

journal_title：FEBS letters

authors： Durchschlag H,Zipper P

更新日期：1988-09-12 00:00:00

abstract：:The four chain agglutinins from Abrus precatorius, Viscum album and Ricinus communis promote depurination of the 28 S rRNA from rabbit reticulocyte ribosomes characteristic of the common ribosome-inactivating proteins (RIPs). These agglutinins inhibited mammalian protein synthesis at nanomolar concentrations but they ...

journal_title：FEBS letters

authors： Citores L,Ferreras JM,Iglesias R,Carbajales ML,Arias FJ,Jiménez P,Rojo MA,Girbés T

更新日期：1993-08-23 00:00:00

abstract：:This study focused on the variations in phosphoinositide metabolism depending upon alphaIIbbeta3-integrin/fibrinogen interaction without previous activation of platelet agonist receptors. We found that adhesion of resting human platelets to immobilized fibrinogen stimulates phosphatidic acid production and a concomita...

journal_title：FEBS letters

authors： Gironcel D,Racaud-Sultan C,Payrastre B,Haricot M,Borchert G,Kieffer N,Breton M,Chap H

更新日期：1996-07-08 00:00:00