In vivo glycosylation suppresses the aggregation of amyloidogenic hen egg white lysozymes expressed in yeast.

abstract：:A short conserved sequence equivalent to the fifth conserved sequence region of alpha-amylases (173_LPDLD, Aspergillus oryzae alpha-amylase) comprising the calcium-ligand aspartate, Asp-175, was identified in the amino acid sequences of several members of the family of (alpha/beta)8-barrel glycosyl hydrolases. Despite...

journal_title：FEBS letters

authors： Janecek S

更新日期：1995-12-11 00:00:00

abstract：:Ribosomes are ribozymes exerting substrate positioning and promoting substrate-mediated catalysis. Peptide-bonds are formed within a symmetrical region, thus suggesting that ribosomes evolved by gene-fusion. Remote interactions dominate substrate positioning at stereochemistry suitable for peptide-bond formation and e...

journal_title：FEBS letters

authors： Baram D,Yonath A

更新日期：2005-02-07 00:00:00

abstract：:The predominance of oxidative stress in many tumour cell environments provides a means to selectively target these cells via protein oxidation. The zinc fingers of transcription factors utilise cysteine thiols for structural zinc coordination. Redox control of DNA binding regulates transcription and therefore the over...

journal_title：FEBS letters

authors： Giles NM,Gutowski NJ,Giles GI,Jacob C

更新日期：2003-01-30 00:00:00

abstract：:Focal adhesion (FA) proteins, kindlin-2 and integrin-linked kinase (ILK), regulate cell adhesion and migration. ILK interacts with and promotes kindlin-2 targeting to FAs. Leu353 and Leu357 in kindlin-2 have been reported to be important for the interaction between kindlin-2 and ILK. However, the binding interface bet...

journal_title：FEBS letters

authors： Guan SY,Chng CP,Ong LT,Tan HF,Alex Law SK,Tan SM

更新日期：2018-01-01 00:00:00

abstract：:The essential role of Factor VIII:C (FVIII:C, anti-hemophilia factor A) as a cofactor for Factor IXa-dependent activation of Factor X has been established. In this paper, we describe that capillary endothelial cells from bovine adrenal medulla express active FVIII:C gene. Accumulation of FVIII:C in conditioned media f...

journal_title：FEBS letters

authors： Banerjee DK,Tavárez JJ,Oliveira CM

更新日期：1992-07-13 00:00:00

abstract：:Modulation of intracellular guanosine 3',5'-bispyrophosphate ((p)ppGpp) level, the effector of the stringent response, is crucial for survival as well as optimal growth of prokaryotes and, thus, for bacterial pathogenesis and dormancy. In Mycobacterium tuberculosis (Mtb), (p)ppGpp synthesis and degradation are carried...

journal_title：FEBS letters

authors： Singal B,Balakrishna AM,Nartey W,Manimekalai MSS,Jeyakanthan J,Grüber G

更新日期：2017-08-01 00:00:00

abstract：:Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...

journal_title：FEBS letters

authors： Berglund A,Backman L,Shanbhag VP

更新日期：1984-06-25 00:00:00

abstract：:Guanylate cyclase activity was measured in disrupted rod outer segments of the toad retina. The experiments showed that cGMP is synthesized from GTP at a rate of 3 +/- 1 nmol/min per mg protein. In darkness this value is largely independent of the Ca2+ concentration, while it is enhanced by flashes of light of increas...

journal_title：FEBS letters

authors： Pepe IM,Panfoli I,Cugnoli C

更新日期：1986-07-14 00:00:00

abstract：:The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

journal_title：FEBS letters

authors： Tate SS

更新日期：1986-01-01 00:00:00

abstract：:Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like ...

journal_title：FEBS letters

authors： van der Goot FG,Pugin J,Hribar M,Fransen L,Dunant Y,De Baetselier P,Bloc A,Lucas R

更新日期：1999-10-22 00:00:00

abstract：:Liposomes can be loaded with weak acids and bases, which exist in solutions in equilibrium with membrane permeable uncharged form, using various gradients across their membranes. Because in some cases the estimated drug concentration in the loaded liposomes exceeds their aqueous solubility we investigated the physical...

journal_title：FEBS letters

authors： Lasic DD,Frederik PM,Stuart MC,Barenholz Y,McIntosh TJ

更新日期：1992-11-09 00:00:00

abstract：:In hydra the neuropeptide head activator (HA) is responsible for head-specific growth and differentiation processes. The effects of HA are mediated by high and low affinity receptors on hydra cells. In the current study HA receptors from a multi-headed mutant of Chlorohydra viridissima were solubilized from the membra...

journal_title：FEBS letters

authors： Christians S,Neubauer KH,Ulrich H

更新日期：1993-01-25 00:00:00

abstract：:Living isolated mitochondria incubated with iron(II) show a major alteration in mitochondrial DNA (mtDNA) conformational forms as assessed by Southern blot analysis of undigested mtDNA. In the presence of iron(II), form I is transformed into form III in a dose-dependent manner. This alteration in mtDNA conformation sh...

journal_title：FEBS letters

authors： Asín J,Pérez-Martos A,Fernández-Silva P,Montoya J,Andreu AL

更新日期：2000-09-01 00:00:00

abstract：:Superoxide reacts with nitric oxide to form peroxynitrite, a potent oxidising agent which may contribute to tissue damage in pathological situations such as inflammation and ischaemia/reperfusion. One mechanism by which oxidative stress damages tissues is the induction of a specific Cyclosporin A-sensitive mitochondri...

journal_title：FEBS letters

authors： Packer MA,Murphy MP

更新日期：1994-05-30 00:00:00

abstract：:A new abnormal hemoglobin, Hb Mito [beta 144(HC 1) Lys----Glu], with an amino acid substitution in the 2,3-DPG pocket, was discovered in a Japanese female. This hemoglobin showed increased oxygen affinity, and decreased organic phosphate and Bohr effects, while the Hill constant n was normal. ...

journal_title：FEBS letters

authors： Harano K,Harano T,Ueda S,Ohkushi T,Imai K

更新日期：1985-11-11 00:00:00

abstract：:The polyomavirus enhancer binding protein 2alpha (PEBP2alpha) is a DNA binding transcriptional regulatory protein that binds conserved sites in the polyomavirus enhancer, mammalian type C retroviral enhancers and T-cell receptor gene enhancers. Binding of PEBP2alpha and homologous proteins to the consensus DNA sequenc...

journal_title：FEBS letters

authors： Pérez-Alvarado GC,Munnerlyn A,Dyson HJ,Grosschedl R,Wright PE

更新日期：2000-03-24 00:00:00

abstract：:The orphan G protein-coupled receptor APJ has been shown to be a coreceptor for human and simian immunodeficiency virus (HIV and SIV) strains. We have determined that some HIV and SIV strains use APJ as a coreceptor to infect the brain-derived NP-2/CD4 cells. Because apelin is an endogenous ligand for the APJ receptor...

journal_title：FEBS letters

authors： Zou MX,Liu HY,Haraguchi Y,Soda Y,Tatemoto K,Hoshino H

更新日期：2000-05-04 00:00:00

abstract：:Plant G proteins of the ROP/RAC family regulate cellular processes including cytoskeletal rearrangement in polar growth. Activation of the ROP molecular switch is triggered by guanine nucleotide exchange factors. Plant-specific RopGEFs are exclusively active on ROPs despite their high homology to animal Rho proteins. ...

journal_title：FEBS letters

authors： Fricke I,Berken A

更新日期：2009-01-05 00:00:00

abstract：:A novel potassium channel beta-subunit (Kv beta 3) was cloned from rat brain being the third member of a Kv beta subunit gene family. It is a protein of 403 amino acid residues with a 68% amino acid sequence homology to Kv beta 1.1. Kv beta 3 is primarily expressed in rat brain having a distribution distinct to those ...

journal_title：FEBS letters

authors： Heinemann SH,Rettig J,Wunder F,Pongs O

更新日期：1995-12-27 00:00:00

abstract：:The toxicity of the beta-amyloid (Abeta) peptide of Alzheimer's disease may relate to its polymerisation state (i.e. fibril content). We have shown previously that plasma lipoproteins, particularly when oxidised, greatly enhance Abeta polymerisation. In the present study the nature of the interactions between both nat...

journal_title：FEBS letters

authors： Stanyer L,Betteridge DJ,Smith CC

更新日期：2002-05-08 00:00:00

abstract：:Kaempferol is a dietary flavonoid that is thought to function as a selective estrogen receptor modulator. In this study, we established that kaempferol also functions as an inverse agonist for estrogen-related receptors alpha and gamma (ERRalpha and ERRgamma). We demonstrated that kaempferol binds to ERRalpha and ERRg...

journal_title：FEBS letters

authors： Wang J,Fang F,Huang Z,Wang Y,Wong C

更新日期：2009-02-18 00:00:00

abstract：:The ability of (S)-alpha-methylproline (alpha-MePro) to stabilise reverse-turn conformations in the peptide hormone bradykinin (BK = Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9) has been investigated. Two BK analogues containing alpha-MePro at position 3 or position 7 were synthesised and their conformations in aqueo...

journal_title：FEBS letters

authors： Welsh JH,Zerbe O,von Philipsborn W,Robinson JA

更新日期：1992-02-10 00:00:00

abstract：:Low density lipoproteins are highly sensitive to oxidation by copper salts, and such peroxidation is accompanied by macrophage scavenger receptor recognition. This study shows that fresh human atherosclerotic material (aneurysms and endarterectomies) can contain detectable amounts of redox active iron and copper that ...

journal_title：FEBS letters

authors： Swain J,Gutteridge JM

更新日期：1995-07-24 00:00:00

abstract：:Cap-independent translation (CIT) occurs at the leader sequences of uncapped plant viral RNAs, but also at a number of normally capped cellular mRNAs and has been correlated with sequence complementarity to 18S rRNA. The ribosomal protein S18 (RPS18) is a component of the small ribosomal subunit and is encoded by thre...

journal_title：FEBS letters

authors： Vanderhaeghen R,De Clercq R,Karimi M,Van Montagu M,Hilson P,Van Lijsebettens M

更新日期：2006-05-15 00:00:00

abstract：:The hypothesis that specific protein kinase C (PKC) isoforms regulate dopamine transporter (DAT) function was tested in Xenopus laevis oocytes expressing human (h)DAT. Activation of conventional PKCs (cPKCs) and novel PKCs (nPKCs) using 10 nM phorbol 12-myristate 13-acetate (PMA) significantly inhibited DAT-associated...

journal_title：FEBS letters

authors： Doolen S,Zahniser NR

更新日期：2002-04-10 00:00:00

abstract：:We have studied the perfusion of the jejunum and ileum in an isolated rat intestine model with flavonoids and hydroxycinnamates and the influence of glycosylation on the subsequent metabolism. Flavone and flavonol glucosides and their corresponding aglycones are glucuronidated during transfer across the rat jejunum an...

journal_title：FEBS letters

authors： Spencer JP,Chowrimootoo G,Choudhury R,Debnam ES,Srai SK,Rice-Evans C

更新日期：1999-09-17 00:00:00

abstract：:Proteins may undergo adaptive structural transitions to accommodate to their cellular milieu and respond to external signals. Modulation of conformational ensembles can rewire the intra- or intermolecular interaction networks and shift between different functional states. Adaptive conformational transitions are associ...

journal_title：FEBS letters

authors： Miskei M,Gregus A,Sharma R,Duro N,Zsolyomi F,Fuxreiter M

更新日期：2017-09-01 00:00:00

abstract：:Oligopeptides derived from the gag polyprotein (Pr55gag) of human immunodeficiency virus type 1 (HIV-1) segment were used to evaluate the extension of the putative binding region for the complex of Pr55gag and the human cytosolic peptidyl prolyl cis/trans isomerase (PPIase) 18 kDa cyclophilin (Cyp18). Five N-terminall...

journal_title：FEBS letters

authors： Schutkowski M,Drewello M,Wöllner S,Jakob M,Reimer U,Scherer G,Schierhorn A,Fischer G

更新日期：1996-10-07 00:00:00

abstract：:A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

journal_title：FEBS letters

authors： Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

更新日期：1993-03-08 00:00:00

abstract：:Siamycin I disrupts growth and quorum sensing in Enterococcus faecalis. Using purified intact protein, we demonstrate here that quorum membrane sensor kinase FsrC is a direct target of siamycin I, reducing pheromone-stimulated autophosphorylation activity by up to 91%. Inhibition was non-competitive with ATP as substr...

journal_title：FEBS letters

authors： Ma P,Nishiguchi K,Yuille HM,Davis LM,Nakayama J,Phillips-Jones MK

更新日期：2011-09-02 00:00:00