Abstract:
:We previously described that NO. leads to destruction of ZnS clusters and release of Zn2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO. leads to an increase of cytoplasmic and nuclear free Zn2+. L929 cells, mouse splenocytes, or rat aorta endothelial cells were labeled with Zinquin-E, a Zn2+-specific fluorophore, and were treated with two different spontaneous NO donors, S-nitrosocysteine or DETA/NO. Both NO donors strongly increased the Zn2+-dependent fluorescence in the cellular cytosol and also in nuclei as compared to controls. NO-dependent Zn2+ release in splenocytes was quantitated by flow cytometry. These results show for the first time, that nitrosative stress mediates intracellular and intranuclear Zn2+ release which may be relevant in altering gene expression patterns.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Berendji D,Kolb-Bachofen V,Meyer KL,Grapenthin O,Weber H,Wahn V,Kröncke KDdoi
10.1016/s0014-5793(97)00150-6subject
Has Abstractpub_date
1997-03-17 00:00:00pages
37-41issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00150-6journal_volume
405pub_type
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