Abstract:
:Nuclear cap-binding protein (CBP) 80/20-dependent translation (CT) is one of the targets for miRNA-mediated gene silencing. Here, we provide evidence that human argonaute 2 (Ago2) competes with CBP80/20 for cap-association, inhibiting CT and thus nonsense-mediated mRNA decay (NMD), which is tightly coupled to CT. Tethering of Ago2, but not of Ago2F2V2 which lacks cap-association activity, to the 3'UTR of PTC-containing mRNA abrogates NMD. Immunoprecipitation using CBP80 antibody reveals that Ago2, but not Ago2F2V2, inhibits the binding of CBP80/20 to cap structure. Our observations provide molecular insight into the cross-talk between miRNA-mediated gene silencing, CT, and NMD.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Choe J,Cho H,Chi SG,Kim YKdoi
10.1016/j.febslet.2011.07.047subject
Has Abstractpub_date
2011-09-02 00:00:00pages
2682-7issue
17eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00592-8journal_volume
585pub_type
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