Abstract:
:In this study, functional proteomics was successfully applied for the characterization of circadian expressed, basic proteins. For this purpose, we have chosen the green model alga Chlamydomonas reinhardtii since its entire nuclear genome is available and it is ideally suited for biochemical enrichment procedures. Proteins from cells harvested during subjective day and night were heparin affinity purified. They were separated by two-dimensional gel electrophoresis suited for basic proteins and analyzed after tryptic digestion by electrospray ionization mass spectrometry. We can show for the first time that the expressions of a protein disulfide isomerase-like protein and a tetratricopeptide repeat protein change in a circadian manner. Interestingly, both proteins are known to be interaction partners in multiprotein complexes including RNA binding proteins.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wagner V,Fiedler M,Markert C,Hippler M,Mittag Mdoi
10.1016/S0014-5793(04)00051-1keywords:
subject
Has Abstractpub_date
2004-02-13 00:00:00pages
129-35issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304000511journal_volume
559pub_type
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