Abstract:
:Linkage between the high-capacity Ca2+-binding protein calsequestrin and the ryanodine receptor is proposed to be essential for proper Ca2+-release during skeletal muscle excitation-contraction coupling. However, no direct biochemical evidence exists showing a connection between these high-molecular-mass complexes in native skeletal muscle membranes. Here, using immunoblot analysis of chemically crosslinked membrane vesicles enriched in triad junctions, we have demonstrated that a very close neighborhood relationship exists between calsequestrin and the ryanodine receptor in both main fiber types. Hence, the luminal Ca2+-reservoir complex appears to be directly coupled to the membrane Ca2+-release complex and oligomerization seems to be of functional importance.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Murray BE,Ohlendieck Kdoi
10.1016/s0014-5793(98)00621-8subject
Has Abstractpub_date
1998-06-16 00:00:00pages
317-22issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00621-8journal_volume
429pub_type
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