Abstract:
:There are two common forms of NRH-quinone oxidoreductase 2 (NQO2) in the human population resulting from SNP rs1143684. One has phenylalanine at position 47 (NQO2-F47) and the other leucine (NQO2-L47). Using recombinant proteins, we show that these variants have similar steady state kinetic parameters, although NQO2-L47 has a slightly lower specificity constant. NQO2-L47 is less stable towards proteolytic digestion and thermal denaturation than NQO2-F47. Both forms are inhibited by resveratrol, but NQO2-F47 shows negative cooperativity with this inhibitor. Thus these data demonstrate, for the first time, clear biochemical differences between the variants which help explain previous biomedical and epidemiological findings.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Megarity CF,Gill JR,Caraher MC,Stratford IJ,Nolan KA,Timson DJdoi
10.1016/j.febslet.2014.02.063subject
Has Abstractpub_date
2014-05-02 00:00:00pages
1666-72issue
9eissn
0014-5793issn
1873-3468pii
S0014-5793(14)00199-9journal_volume
588pub_type
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