Abstract:
:A heat-stable activator for ADP-ribosylation factor (ARF)-dependent phospholipase D (PLD) was purified to near homogeneity from rat kidney cytosol by a sequential column chromatography. The purified activator has a molecular mass of 23 kDa on SDS-PAGE. Using a partially purified ARF-dependent PLD from rat kidney, the activator synergistically stimulates PLD with ARF in time- and dose-dependent manner. In the absence of ARF, the activator has little or no effect. The purified activator also stimulates PLD under several conditions including permeabilized cell system, suggesting that the activator is a physiologically relevant regulator of PLD.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Akisue T,Jinnai H,Hitomi T,Miwa N,Yoshida K,Nakamura Sdoi
10.1016/s0014-5793(97)01611-6subject
Has Abstractpub_date
1998-01-23 00:00:00pages
108-12issue
1eissn
0014-5793issn
1873-3468pii
S0014579397016116journal_volume
422pub_type
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