Abstract:
:Replication of Brucella inside eukaryotic cells is essential for pathogenesis, and successful infection requires rapid adaptation to the intracellular milieu. Close relatives of Brucella use the two-component system FixLJ to survive inside the host. We aimed to identify a homologous sensor in Brucella abortus. A predicted protein with transmembrane and conserved histidine kinase domains was identified as the Fix-like Brucella sensor, FlbS. Although it lacks the PAS domain, recombinant FlbS binds haem in vitro. An internal in-frame deletion in flbS severely decreased B. abortus survival inside professional and non-professional phagocytes. This phenotype was reverted by genetic complementation. These results indicate the critical role of this haemoprotein in the intracellular lifestyle of Brucella.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Roset MS,Almirón MAdoi
10.1016/j.febslet.2013.07.047subject
Has Abstractpub_date
2013-09-17 00:00:00pages
3102-7issue
18eissn
0014-5793issn
1873-3468pii
S0014-5793(13)00609-1journal_volume
587pub_type
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