Restoration of hydroperoxide-dependent lipid peroxidation by 3-methylcholanthrene induction of cytochrome P-448 in hepatoma microsomes.

abstract：:Depletion of mitochondrial DNA (mtDNA) causes defects in respiratory activity and energy production. Recent studies have shown mitochondria to exist primarily as reticular networks, having tubular cristae. Using fluorescence microscopy and transmission electron microscopy, we have examined mitochondrial morphology and...

journal_title：FEBS letters

authors： Gilkerson RW,Margineantu DH,Capaldi RA,Selker JM

更新日期：2000-05-26 00:00:00

abstract：:Fibroblasts were exposed to various concentrations of hydrogen peroxide and the removal of oxidized proteins was followed by determining protein-bound carbonyls. Fibroblasts are able to increase the turnover of metabolically radiolabeled proteins after treatment with hydrogen peroxide. It was demonstrated for the firs...

journal_title：FEBS letters

authors： Sitte N,Merker K,Grune T

更新日期：1998-12-04 00:00:00

abstract：:Flavonoid 3'-hydroxylase (F3'H) and flavonoid 3',5'-hydroxylase (F3'5'H) are cytochrome P450 enzymes and determine the B-ring hydroxylation pattern of flavonoids by introducing hydroxyl groups at the 3'- or the 3'- and 5'-position, respectively. Sequence identity between F3'H and F3'5'H is generally low since their di...

journal_title：FEBS letters

authors： Seitz C,Ameres S,Forkmann G

更新日期：2007-07-24 00:00:00

abstract：:In order to study the transactivational property of the X gene in the methylotropic yeast Pichia pastoris, a Rous sarcoma virus-chloramphenicol acetyltransferase (RSV-CAT) cassette was co-transformed and integrated into the host yeast strain as a reporter which showed an overwhelming CAT activity. Immunoprecipitation ...

journal_title：FEBS letters

authors： Lal SK,Kumar V

更新日期：1999-07-30 00:00:00

abstract：:Two synthetic photoactive azidoarylphosphatidylcholines were used to investigate the level of interaction between D-beta-hydroxybutyrate apodehydrogenase (apoBDH), an amphipathic membrane protein, with the hydrophobic domain of phospholipids. The two synthetic lecithins, PL I (1-myristoyl-2-12-N-(4-azido-2-nitrophenyl...

journal_title：FEBS letters

authors： el Kebbaj MS,Berrez JM,Lakhlifi T,Morpain C,Latruffe N

更新日期：1985-03-11 00:00:00

abstract：:Stimulated production of reactive oxygen species (ROS) by plasma membrane-associated nicotinamide adenine dinucleotide phosphate oxidases (Nox) in non-phagocytic cells regulates a number of biological processes including growth, vessel tone, and oxygen sensing. The purpose of this study was to investigate H(2)O(2)-sti...

journal_title：FEBS letters

authors： Colston JT,de la Rosa SD,Strader JR,Anderson MA,Freeman GL

更新日期：2005-04-25 00:00:00

abstract：:Residues 370-383 (helix C) of the human nerve growth factor receptor (NGF-R) are highly similar to the sequence of the 14 residue wasp toxin, mastoparan. Both regions are predicted to form amphiphilic alpha-helices, as is the amino-terminal region of the third intracytoplasmic loop (i3) of the beta 2-adrenergic recept...

journal_title：FEBS letters

authors： Feinstein DL,Larhammar D

更新日期：1990-10-15 00:00:00

abstract：:Maculatin 1.1 is an antimicrobial peptide isolated from the Australian tree frog Litoria genimaculata that adopts an amphipathic, alpha-helical structure in solution. Its orientation and conformation when incorporated to pre-formed DMPG (1,2-dimyristoyl-sn-glycero-3-phosphoglycerol) and DMPC (1,2-dimyristoyl-sn-glycer...

journal_title：FEBS letters

authors： Chia CS,Torres J,Cooper MA,Arkin IT,Bowie JH

更新日期：2002-02-13 00:00:00

abstract：:CXCR4 (fusin) is a chemokine receptor which is involved as a coreceptor in gp120 binding to the cell surface. In this study we provide evidence that binding of gp120 triggers CXCR4 recruitment to glycosphingolipid-enriched microdomains. Scanning confocal microscopy showed a nearly complete localization of CXCR4 within...

journal_title：FEBS letters

authors： Sorice M,Garofalo T,Misasi R,Longo A,Mattei V,Sale P,Dolo V,Gradini R,Pavan A

更新日期：2001-09-28 00:00:00

abstract：:In fat-cells, insulin increases synergistically the inhibitory effects of adenosine and prostaglandin E2 on adenylyl cyclase activity. When the endogenous production of these feedback inhibitors is suppressed, insulin is no more active in conditions where glycosyl inositol-phosphate which is a putative mediator of its...

journal_title：FEBS letters

authors： Martiny L,Dib K,Haye B,Corrèze C,Jacquemin C,Lambert B

更新日期：1991-07-29 00:00:00

abstract：:A gene encoding a single-chain antibody fragment directed against digoxin (named 1C10 scFv) was cloned in two expression systems. For this purpose, a new baculovirus transfer cassette fully compatible with the procaryotic pHEN vector was constructed. Baculovirus production led to higher yield than did Escherichia coli...

journal_title：FEBS letters

authors： Lemeulle C,Chardès T,Montavon C,Chaabihi H,Mani JC,Pugnière M,Cerutti M,Devauchelle G,Pau B,Biard-Piechaczyk M

更新日期：1998-02-20 00:00:00

abstract：:N-Oligosaccharyltransferase catalyzes the N-glycosylation of asparagine residues of nascent polypeptide chains in the endoplasmic reticulum, a pathway highly conserved in all eukaryotes. An enzymatically active complex was isolated from microsomal membranes from Saccharomyces cerevisiae, which is composed of four prot...

journal_title：FEBS letters

authors： Knauer R,Lehle L

更新日期：1994-05-09 00:00:00

abstract：:Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene...

journal_title：FEBS letters

authors： Müller KM,Arndt KM,Strittmatter W,Plückthun A

更新日期：1998-01-30 00:00:00

abstract：:The Escherichia coli K12 mutant gene, asnS40, coding for asparaginyl-tRNA synthetase (AsnRS) in the temperature-sensitive strain HO202, was isolated from genomic DNA using the Polymerase Chain Reaction. DNA sequencing revealed that the mutant enzyme differs from the wild-type AsnRS by two amino acids, but only the P23...

journal_title：FEBS letters

authors： Madern D,Anselme J,Härtlein M

更新日期：1992-03-24 00:00:00

abstract：:In the present study, we have explored the impact of deleting a single allele of PDK1 in T cell progenitors on alpha/beta and gamma/delta T cell development. The data show that deleting a single allele of PDK1 allows differentiation of alpha/beta T cells but prevents their proliferative expansion in the thymus. Accord...

journal_title：FEBS letters

authors： Kelly AP,Hinton HJ,Clarke RG,Cantrell DA

更新日期：2006-04-03 00:00:00

abstract：:Saturable binding sites for the labelled calcium antagonist (+/-)[3H]nimodipine were found in guinea-pig hind limb skeletal muscle homogenates. Binding sites were enriched in a microsomal pellet by differential centrifugation of the homogenate. [3H]Nimodipine binding (Kd = 1.5 +/- 0.03 nM, Bmax = 2.1 +/- 0.25 pmol/pro...

journal_title：FEBS letters

authors： Ferry DR,Glossmann H

更新日期：1982-11-08 00:00:00

abstract：:In higher eukaryotes, nine aminoacyl-tRNA synthetases form a multienzyme complex also comprising the three auxiliary proteins p18, p38 and p43, of apparent molecular masses of 18, 38 and 43 kDa. The function of these proteins, invariably found associated to the synthetase components of the complex, is unknown. In orde...

journal_title：FEBS letters

authors： Quevillon S,Mirande M

更新日期：1996-10-14 00:00:00

abstract：:Specific activity was compared between wild-type (WT) neutral protease from Bacillus stearothermophilus and mutant protease (M1; Gly144 replaced by Ala144) with enhanced thermostability. When casein was used as a substrate, M1 showed 1.5-times higher specific activity than that of WT. In contrast, the specific activit...

journal_title：FEBS letters

authors： Takagi M,Imanaka T

更新日期：1989-08-28 00:00:00

abstract：:Studies of the inhibition of elastases at a molecular level have resulted in the identification of protected dipeptides which are reversible and highly specific inhibitors of human leucocyte elastase (HLE). These have been further developed by increasing their hydrophilicity and potency to give a new family of elastas...

journal_title：FEBS letters

authors： Hassall CH,Johnson WH,Kennedy AJ,Roberts NA

更新日期：1985-04-22 00:00:00

abstract：:A structure of residues 1-177 of the cyclophilin domain of a large divergent cyclophilin from the filarial nematode parasite Brugia malayi has been crystallised and solved in two different crystal forms. The active site has a similar structure to that of human cyclophilin A. Two of the 13 residues important in forming...

journal_title：FEBS letters

authors： Taylor P,Page AP,Kontopidis G,Husi H,Walkinshaw MD

更新日期：1998-03-27 00:00:00

abstract：:It is increasingly clear that microRNAs play a crucial role in tumorigenesis. Recently, emerging evidence suggested that miR-26a is aberrantly expressed in tumor tissues. In our study, frequent down-regulation of miR-26a was observed in 10 human bladder cancer tissues. Forced expression of miR-26a in the bladder cance...

journal_title：FEBS letters

authors： Lin Y,Chen H,Hu Z,Mao Y,Xu X,Zhu Y,Xu X,Wu J,Li S,Mao Q,Zheng X,Xie L

更新日期：2013-08-02 00:00:00

abstract：:Several components of translation, e.g. eIF4E and PKR, are implicated in cancer. The e-subunit (p48) of mammalian initiation factor 3 is encoded by the Int6 gene, a common site for integration of the mouse mammary tumor virus genome, leading to the production of a truncated eukaryotic initiation factor-3e (eIF3e). Sta...

journal_title：FEBS letters

authors： Mayeur GL,Hershey JW

更新日期：2002-03-06 00:00:00

abstract：:α-Helical membrane-active antimicrobial peptides (AMPs) are known to act via a range of mechanisms, including the formation of barrel-stave and toroidal pores and the micellisation of the membrane (carpet mechanism). Different mechanisms imply that the peptides adopt different 3D structures when bound at the water-mem...

journal_title：FEBS letters

authors： van den Bergen G,Stroet M,Caron B,Poger D,Mark AE

更新日期：2020-03-01 00:00:00

abstract：:The conversion of the cellular prion protein into the beta-sheet-rich scrapie prion protein is thought to be the key step in the pathogenesis of prion diseases. To gain insight into this structural conversion, we analyzed the intrinsic structural propensity of the amino acid sequence of the murine prion C-terminal dom...

journal_title：FEBS letters

authors： Jamin N,Coïc YM,Landon C,Ovtracht L,Baleux F,Neumann JM,Sanson A

更新日期：2002-10-09 00:00:00

abstract：:We have investigated the effect of botulinum neurotoxin (BoNT) C1 light chain (LC) on insulin exocytosis from the clonal beta-cell line HIT-T15. In streptolysin-O permeabilized cells, the beta-cell impermeant BoNT C1 cleaved mainly syntaxin 1 and inhibited Ca2+ as well as GTPgammaS induced exocytosis. To study the eff...

journal_title：FEBS letters

authors： Land J,Zhang H,Vaidyanathan VV,Sadoul K,Niemann H,Wollheim CB

更新日期：1997-12-08 00:00:00

abstract：:Several lines of evidence indicate that selenoproteins mainly act as cellular antioxidants. Here, we test this idea comparing the sensitivity to oxidative stress (paraquat and hydrogen peroxide) between wild type and heterozygous flies for the selenophosphate synthetase selD(ptuf) mutation. Whereas under normal labora...

journal_title：FEBS letters

authors： Morey M,Serras F,Corominas M

更新日期：2003-01-16 00:00:00

abstract：:Transcriptional responses to growth in high environmental calcium concentrations were characterized and compared between wild-type and mutant Arabidopsis plants containing a knockout mutation in the gene encoding a cyclic nucleotide-gated channel (CNGC2). We show that the transcriptional profile of cngc2 plants grown ...

journal_title：FEBS letters

authors： Chan CW,Wohlbach DJ,Rodesch MJ,Sussman MR

更新日期：2008-03-19 00:00:00

abstract：:Oncogenic mutations disrupt the regulatory circuits that govern cell function, enabling tumor cells to undergo de-regulated mitogenesis, to resist to pro-apoptotic insults, and to invade through tissue boundaries. Cancer cell biology has played a crucial role in elucidating the signaling mechanisms by which oncogenic ...

journal_title：FEBS letters

authors： Giancotti FG

更新日期：2014-08-19 00:00:00

abstract：:Undifferentiated rat pheochromocytoma PC12 cells were voltage clamped using the whole cell technique. After blockade of outward currents, calcium currents were elicited from -40 and -100 mV. A subpopulation of cells displayed only one current component activated at -10 mV and slowly decaying. In other cells this curre...

journal_title：FEBS letters

authors： Janigro D,Maccaferri G,Meldolesi J

更新日期：1989-09-25 00:00:00

abstract：:As part of a search for selective, mechanism-based covalent inhibitors of human pancreatic α-amylase we describe the chemoenzymatic synthesis of the disaccharide analog α-glucosyl epi-cyclophellitol, demonstrate its stoichiometric reaction with human pancreatic α-amylase and evaluate the time dependence of its inhibit...

journal_title：FEBS letters

authors： Caner S,Zhang X,Jiang J,Chen HM,Nguyen NT,Overkleeft H,Brayer GD,Withers SG

更新日期：2016-04-01 00:00:00