Identification of a conserved protein motif in a group of growth factor receptors.

abstract：:Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant D...

journal_title：FEBS letters

authors： Ho T,Wang H,Pallett D,Dalmay T

更新日期：2007-07-10 00:00:00

abstract：:A single chain ribosome-inactivating protein with RNA N-glycosidase activity, here named Dianthin 29, was isolated from leaves of Dianthus barbatus L. Incubation of intact Escherichia coli ribosomes with Dianthin 29 and subsequent aniline treatment of the isolated rRNA releases a rRNA fragment of 243 nucleotides from ...

journal_title：FEBS letters

authors： Prestle J,Hornung E,Schönfelder M,Mundry KW

更新日期：1992-02-10 00:00:00

abstract：:Cytochrome bd is one of the two terminal ubiquinol oxidases in the respiratory chain of Escherichia coli catalyzing reduction of O2 to H2O. The enzyme is expressed under low oxygen tension; due to high affinity for O2 it is isolated mainly as a stable oxygenated complex. Direct measurement of O2 binding to heme d in t...

journal_title：FEBS letters

authors： Belevich I,Borisov VB,Konstantinov AA,Verkhovsky MI

更新日期：2005-08-29 00:00:00

abstract：:Under hypobaric hypoxia, antioxidant defenses of the heart are stressed by the enhanced production of ROS. Mammalian heart acclimatizes to hypoxia through altered gene expression, which we studied in murine heart exposed to 10h of acute hypobaric hypoxia (AHH), equivalent to 15000ft, using cDNA arrays. Functional clas...

journal_title：FEBS letters

authors： Karar J,Dolt KS,Mishra MK,Arif E,Javed S,Pasha MA

更新日期：2007-10-02 00:00:00

abstract：:In bovine adrenocortical cells, the fatty acyl chains of the phospholipids have been identified in the membranes of the different cell compartments: plasma membranes, Golgi complex and coated vesicle membranes. An increase in the total number of unsaturation in the fatty acid is demonstrated in the coated vesicle memb...

journal_title：FEBS letters

authors： de Paillerets C,Bomsel M,Weintraub H,Pépin D,Alfsen A

更新日期：1987-07-13 00:00:00

abstract：:Proteorhodopsin (PR), a retinal-containing seven transmembrane helix protein, functions as a light-driven proton pump. Using PCR, we isolated 18 PR variants originating from the surface of the Arctic Ocean. Their absorption maxima were between 517 and 546 nm at pH 7. One of the isolates turned out to be identical to G...

journal_title：FEBS letters

authors： Jung JY,Choi AR,Lee YK,Lee HK,Jung KH

更新日期：2008-05-28 00:00:00

abstract：:When a cell divides prior to completion of DNA replication, serious DNA damage may occur. Thus, in addition to accuracy, the processivity of the replication forks is important. DNA synthesis at replication forks should be completed in time, and forks overcome aberrant structures on the template DNA, including damaged ...

journal_title：FEBS letters

authors： Hizume K,Araki H

更新日期：2019-07-01 00:00:00

abstract：:The predicted Exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient Siberian permafrost sample. The protein bacteriorhodopsin from Exiguobacterium sibiricum (ESR) encoded by this gene was expressed in Escherichia coli membrane. ESR bound all-trans-retinal and displayed an absorbance maximum at 5...

journal_title：FEBS letters

authors： Petrovskaya LE,Lukashev EP,Chupin VV,Sychev SV,Lyukmanova EN,Kryukova EA,Ziganshin RH,Spirina EV,Rivkina EM,Khatypov RA,Erokhina LG,Gilichinsky DA,Shuvalov VA,Kirpichnikov MP

更新日期：2010-10-08 00:00:00

abstract：:Annexin-I is a calcium dependent phospholipid binding and phospholipase A2 (PLA2) inhibitory protein. A 'substrate depletion' model has been proposed for the mechanism of PLA2 inhibition by annexin-I in studies with 14 to 18 kDa PLA2s. Herein, we have studied the inhibition mechanism using 100 kDa cytosolic PLA2 from ...

journal_title：FEBS letters

authors： Kim KM,Kim DK,Park YM,Kim CK,Na DS

更新日期：1994-05-02 00:00:00

abstract：:For more than three decades, RNA recombination remained a puzzle and has only begun to be solved in the last few years. The available data provide evidence for a variety of RNA recombination mechanisms. Non-homologous recombination seems to be the most common for RNA. Recent experiments in both the in vitro and the in...

journal_title：FEBS letters

authors： Chetverin AB

更新日期：1999-10-22 00:00:00

abstract：:The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp....

journal_title：FEBS letters

authors： Znamenskaya LV,Gabdrakhmanova LA,Chernokalskaya EB,Leshchinskaya IB,Hartley RW

更新日期：1995-01-02 00:00:00

abstract：:Caveolin is a protein present in membrane specializations termed caveolae where it may play a structural role. Previous studies on the cDNA sequence of chicken caveolin demonstrated that it is an integral membrane protein without significant homology to any known protein. The cDNA sequence of human lung caveolin is pr...

journal_title：FEBS letters

authors： Glenney JR Jr

更新日期：1992-12-07 00:00:00

abstract：:Incubation of human thrombocytes in the presence of [3H]palmitic acid leads to incorporation of this fatty acid into the alpha subunit of Gs as described [Linder et al., Proc. Natl. Acad. Sci. USA 90 (1993) 3675-3679; Degtyarev et al., Biochemistry 32 (1993) 8057-8061] but also into the catalyst of adenylyl cyclase wh...

journal_title：FEBS letters

authors： Mollner S,Beck K,Pfeuffer T

更新日期：1995-09-11 00:00:00

abstract：:Using a panel of monoclonal antibodies, it has previously been demonstrated that the cytosol of nucleated red cells (trout and turkey) contains a protein similar to arrestin, a soluble protein found so far only in the photosensitive cells and which, by binding to photoexcited rhodopsin, inhibits the phototransduction ...

journal_title：FEBS letters

authors： Scheuring U,Franco M,Fievet B,Guizouarn H,Mirshahi M,Faure JP,Motais R

更新日期：1990-12-10 00:00:00

abstract：:Motile flagella are crucial for human fertility and embryonic development. The distal tip of the flagellum is where growth and intra-flagellar transport are coordinated. In most model organisms, but not all, the distal tip includes a 'singlet region', where axonemal doublet microtubules (dMT) terminate and only comple...

journal_title：FEBS letters

authors： Zabeo D,Croft JT,Höög JL

更新日期：2019-05-01 00:00:00

abstract：:Several recombinant proteins in inclusion bodies expressed in Escherichia coli have been measured by Fourier transform infrared and solid-state nuclear magnetic resonance spectra to provide the secondary structural characteristics of the proteins from hyperthermophilic archaeon Pyrococcus horikoshii OT3 (hyperthermoph...

journal_title：FEBS letters

authors： Umetsu M,Tsumoto K,Ashish K,Nitta S,Tanaka Y,Adschiri T,Kumagai I

更新日期：2004-01-16 00:00:00

abstract：:WW domains harbor substrates containing proline-rich motifs, but the substrate specificity and binding mechanism remain elusive for those WW domains less amenable for structural studies, such as human WWP2 (hWWP2). Herein we have employed multiple techniques to investigate the second WW domain (WW2) in hWWP2. Our resu...

journal_title：FEBS letters

authors： Jiang J,Wang N,Jiang Y,Tan H,Zheng J,Chen G,Jia Z

更新日期：2015-07-08 00:00:00

abstract：:Two highly contrasted images depict genomes: at first sight, genes appear to be distributed randomly along the chromosome. In contrast, their organisation into operons (or pathogenicity islands) suggests that, at least locally, related functions are in physical proximity. Analysis of the codon usage bias in orthologou...

journal_title：FEBS letters

authors： Rocha EP,Sekowska A,Danchin A

更新日期：2000-06-30 00:00:00

abstract：:Leonor Michaelis spent the years of 1922-1926 as Professor of Biochemistry of the Aichi Medical College (now Graduate School of Medicine, Nagoya University) in Nagoya, Japan. Michaelis succeeded in gathering many bright young biochemists from all over Japan into his laboratory, and made tremendous contributions to the...

journal_title：FEBS letters

authors： Nagatsu TT

更新日期：2013-09-02 00:00:00

abstract：:Analogues of S-prenylated cysteine like N-acetyl-S-trans,trans-farnesyl-L-cysteine (AFC) have previously been shown to inhibit the carboxyl methylation of proteins carrying a C-terminal S-prenylated cysteine residue and to block the endotoxin-activated serum-elicited chemotactic response of mouse macrophages. Here, we...

journal_title：FEBS letters

authors： Scheer A,Gierschik P

更新日期：1993-03-15 00:00:00

abstract：:Three mutant polypeptides of the type I 37-residue winter flounder 'antifreeze' protein have been synthesized. All four threonine residues in the native peptide were been mutated to serine, valine and glycine respectively and two additional salt bridges were incorporated into the sequences in order to improve aqueous ...

journal_title：FEBS letters

authors： Haymet AD,Ward LG,Harding MM,Knight CA

更新日期：1998-07-03 00:00:00

abstract：:Following the biophysical analysis of plant K+ channels in their natural environment, three members from the green branch of the evolutionary tree of life KAT1, AKT1, and KST1 have recently been identified on the molecular level. Among them, we focussed on the expression and characterization of the Arabidopsis thalian...

journal_title：FEBS letters

authors： Marten I,Gaymard F,Lemaillet G,Thibaud JB,Sentenac H,Hedrich R

更新日期：1996-02-19 00:00:00

abstract：:The Xenopus laevis oocyte has endogenous sites that bind muscarinic agonists, which have been pharmacologically characterized as M3 and/or M1 receptor subtypes. In order to define the molecular identify of the receptor protein we have analyzed a Xenopus oocyte cDNA library and cloned a 2.9 kb cDNA fragment encoding a ...

journal_title：FEBS letters

authors： Herrera L,Carvallo P,Antonelli M,Olate J

更新日期：1994-09-26 00:00:00

abstract：:Two human mammary carcinoma cell variants, MCF-7/AZ and MCF-7/6, show the same composition in their glycosphingolipid-enriched microdomain (GEM) with regard to globo-series structures Gb3, Gb4, Gb5, monosialyl-Gb5, GM2, and cSrc and FAK. Both variants are non-invasive into collagen gel layer, and showed similar motili...

journal_title：FEBS letters

authors： Steelant WF,Kawakami Y,Ito A,Handa K,Bruyneel EA,Mareel M,Hakomori S

更新日期：2002-10-30 00:00:00

abstract：:The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re...

journal_title：FEBS letters

authors： Vojta L,Soll J,Bölter B

更新日期：2007-06-12 00:00:00

abstract：:Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunop...

journal_title：FEBS letters

authors： Wu X,Zhou Y,Zhang K,Liu Q,Guo D

更新日期：2008-06-25 00:00:00

abstract：:Protein kinase CK2 is composed of two regulatory beta-subunits and two catalytic alpha- or alpha'-subunits. To analyse these subunits individually we generated antibodies against unique peptides derived from the alpha-, alpha'- and beta-subunit. Immunofluorescence studies with these antibodies revealed the presence of...

journal_title：FEBS letters

authors： Faust M,Schuster N,Montenarh M

更新日期：1999-11-26 00:00:00

abstract：:Type II restriction endonucleases (REases) exist in multiple oligomeric forms. The tetrameric REases have two DNA binding interfaces and must synapse two recognition sites to achieve cleavage. It was hypothesised that binding of two recognition sites by tetrameric enzymes contributes to their fidelity. Here, we experi...

journal_title：FEBS letters

authors： Zaremba M,Sasnauskas G,Siksnys V

更新日期：2012-09-21 00:00:00

abstract：:The rotary motion in response to ATP hydrolysis of the ring of c subunits of the membrane portion, F(o), of ATP synthase, F(o)F(1), is still under contention. It was studied with EF(o)EF(1) (Escherichia coli) using microvideography with a fluorescent actin filament. To overcome the limited specificity of actin attachm...

journal_title：FEBS letters

authors： Pänke O,Gumbiowski K,Junge W,Engelbrecht S

更新日期：2000-04-21 00:00:00

abstract：:C1qTNF-related proteins (CTRPs) are involved in diverse processes including metabolism, inflammation host defense, apoptosis, cell differentiation, autoimmunity, hibernation, and organogenesis. However, the physiological role of CTRP6 remains poorly understood. Here we demonstrate that the globular domain of CTRP6 med...

journal_title：FEBS letters

authors： Lee W,Kim MJ,Park EJ,Choi YJ,Park SY

更新日期：2010-03-05 00:00:00