Abstract:
:Using a panel of monoclonal antibodies, it has previously been demonstrated that the cytosol of nucleated red cells (trout and turkey) contains a protein similar to arrestin, a soluble protein found so far only in the photosensitive cells and which, by binding to photoexcited rhodopsin, inhibits the phototransduction process. The role of this arrestin-like protein in non-photosensitive cells is questionable. In this report we present evidence that partially purified red blood cell arrestin (RBC arrestin) behaves functionally like bovine retinal arrestin: it binds to phosphorylated bovine rhodopsin only when this receptor has been photoactivated. Thus RBC arrestin and bovine retinal arrestin are closely related both structurally and functionally. By analogy with the function of retinal arrestin, it is proposed that RBC arrestin is involved in desensitization of membrane transport proteins and/or adrenergic receptors.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Scheuring U,Franco M,Fievet B,Guizouarn H,Mirshahi M,Faure JP,Motais Rdoi
10.1016/0014-5793(90)80540-ysubject
Has Abstractpub_date
1990-12-10 00:00:00pages
192-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(90)80540-Yjournal_volume
276pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Many receptors coupled to inhibitory Go/Gi-type G proteins often also produce stimulatory signals like Ca2+ mobilisation. When expressed in CHO cells the alpha2-adrenoceptor subtypes alpha2A, alpha2B and alpkha2C mobilised Ca2+. These responses were strongly reduced by the P2Y-purinoceptor antagonist suramin. A large ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00664-4
更新日期:1998-07-03 00:00:00
abstract::Several mRNAs are known to be targeted to dendrites in hippocampal neurons. In this study, we show that brain-derived neurotrophic factor (BDNF) mRNA has two distinct cis-acting dendritic targeting elements in the short 3' untranslated region (UTR): a constitutive element and an activity-dependent one. Moreover, delet...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.040
更新日期:2010-08-04 00:00:00
abstract::Using a cDNA library from bovine adrenal medulla, and, subsequently, a bovine genomic library, we have isolated the gene coding for a non inactivating potassium channel. This gene encodes a 597-amino acid protein which we have called BAK5 as its sequence is very similar to members of Kv1.5 potassium channel family. Ne...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01117-6
更新日期:1994-11-07 00:00:00
abstract::The covalent binding of pyridoxal 5'-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H] PLP, and the Schif...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80073-p
更新日期:1992-02-24 00:00:00
abstract::HERC proteins are characterized by having one or more RCC1-like domains as well as a C-terminal HECT domain in their amino acid sequences. This has led researchers to suggest that they may act as both guanine nucleotide exchange factors and E3 ubiquitin ligases. Here we describe a physical interaction between the HECT...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00205-9
更新日期:2003-03-27 00:00:00
abstract::Organisms using the thiosulfate-oxidizing Sox enzyme system fall into two groups: group 1 forms sulfur globules as intermediates (Allochromatium vinosum), group 2 does not (Paracoccus pantotrophus). While several components of their Sox systems are quite similar, i.e. the proteins SoxXA, SoxYZ and SoxB, they differ by...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.03.020
更新日期:2009-04-17 00:00:00
abstract::To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subun...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.090
更新日期:2004-09-24 00:00:00
abstract::We developed a novel method to quantitatively measure GTP gamma S binding to specific G proteins in crude membranes using G-protein antibodies. The basic strategy was that the materials were initially incubated with [35S]GTP gamma S at 37 degrees C. After 4 degrees C incubation in the wells of an ELISA plate precoated...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80878-k
更新日期:1992-06-29 00:00:00
abstract::Using RT-PCR we distinguished mRNAs for all known G-protein coupled serotonin receptors expressed in various rat and porcine blood vessels. Nearly all vessels expressed 5HT1D beta, 5-HT2A, 5-HT2B, 5-HT4, and 5-HT7 receptor mRNA to different extents. New splice variants of the porcine 5-HT4 receptor were observed. Simi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00828-w
更新日期:1995-08-21 00:00:00
abstract::Telomerase is an almost universal cancer target that consists minimally of a core protein human telomerase reverse transcriptase (hTERT) and a RNA component human telomerase RNA (hTR). Some inhibitors of this enzyme are thought to function by the covalent binding to one or several cysteine residues; however, this inhi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12589
更新日期:2017-03-01 00:00:00
abstract::In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.08.021
更新日期:2014-10-16 00:00:00
abstract::Disulfide bridges were introduced into CrylAa, a Bacillus thuringiensis lepidopteran toxin, to stabilize different protein domains including domain I alpha-helical regions thought to be involved in membrane integration and permeation. Bridged mutants could not form functional ion channels in lipid bilayers in the oxid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00626-1
更新日期:1997-06-30 00:00:00
abstract::The tissue inhibitors of matrix metalloproteinases (MMPs), TIMP-1 and TIMP-2, are also angiogenesis inhibitors. Cathepsin B and MMPs are found at sites of neovascularization in pathologies such as cancer and osteoarthritis. Treatment of TIMP-1, TIMP-2, and of a mixture of both inhibitors from human articular chondrocy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00897-2
更新日期:1999-07-23 00:00:00
abstract::Posttranslational modifications of circadian oscillator components are crucial for the generation of circadian rhythms. Among those phosphorylation plays key roles ranging from regulating degradation, complex formation, subcellular localization and activity. Although most of the known clock proteins are phosphoprotein...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.02.038
更新日期:2011-05-20 00:00:00
abstract::A 32 kDa phospholipase A2 inhibitory protein was isolated from pig thyroid gland after calcium precipitation and fast protein liquid anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis revealed the purity of the protein. The protein activity was assessed by the inhibition of pancreatic phospholipase ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81273-0
更新日期:1988-08-01 00:00:00
abstract::A cDNA clone encoding the sequence of human pancreatic lipase (HPL) was subcloned into the baculovirus transfer vector pVL1392 and used in co-transfection of Spodoptera frugiperda (Sf9) insect cells with wild-type Autographa californica nuclear polyhedrosis virus (AcNPV) DNA. A single recombinant protein (50 kDa) secr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81044-z
更新日期:1993-07-19 00:00:00
abstract::When a high density of alpha1-subunit glycine receptor (GlyR) is expressed in Xenopus oocytes, two populations of channels can be distinguished according to their apparent affinity for glycine which differs 5- to 6-fold. To compare the open pore diameter of these channels, the relative permeability of formate with res...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00986-1
更新日期:1997-09-01 00:00:00
abstract::Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01752-x
更新日期:2000-07-07 00:00:00
abstract::RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.02.052
更新日期:2012-04-05 00:00:00
abstract::The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80831-3
更新日期:1990-12-17 00:00:00
abstract::While negative effect of smoking on the resistance to viral infections was known, the underlying mechanisms remained unclear. Here we report that products of cigarette smoking compromise the cellular anti-viral defenses by inhibiting the signaling induced by Type I interferon (IFN). Cigarette smoking condensate (but n...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.013
更新日期:2008-09-22 00:00:00
abstract::Rats were infused intravenously with a bolus of mevalonolactone or cholesterol-rich chylomicron and microsomes prepared from the livers at times up to 2 h after the infusion. Acyl-CoA cholesterol acyltransferase (ACAT) activity was measured in the microsomes under conditions that define the activity state of the enzym...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80410-4
更新日期:1983-08-08 00:00:00
abstract::Internal promoters of some genes transcribed by RNA polymerase III (e.g. tRNA genes, adenovirus VA1 RNA gene, human retroposons of the Alu family) contain a conserved sequence element, B-box, interacting with basal transcription factor TFIIIC2 which initiates assembly of the full transcription complex on the genes, an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00353-5
更新日期:1996-05-13 00:00:00
abstract::The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTP gamma[35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' alpha-subunit. Cross-reaction with a 24-kDa protein is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81251-i
更新日期:1990-09-17 00:00:00
abstract::Activation of T lymphocytes results in an increase in intracellular Ca2+ due in large part to influx of extracellular Ca2+. Using the patch clamp technique, an inward current in Jurkat T lymphocytes was observed upon depolarization from a holding potential of -90 mV but not from -60 mV. This whole-cell current was ins...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80926-8
更新日期:1992-11-09 00:00:00
abstract::EVH1 (enabled VASP (vasodilator-stimulated protein) homology 1)/WH1 (WASP (Wiskott-Aldrich syndrome protein) homology 1) domains, present in Ena VASP and WASP, are protein interaction modules specialised in binding proline-rich ligands. An EVH1/WH1 domain is here identified in the recently cloned SMIF protein, a key p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02751-5
更新日期:2002-05-22 00:00:00
abstract::Three homologous aspartate aminotransferases with virtually identical spatial structures and pairwise amino acid sequence identities of > 40% differ markedly with respect to the yield of renaturation upon dilution from 6 M guanidine hydrochloride (mitochondrial < cytosolic < Escherichia coli). The enzymes also respond...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01406-3
更新日期:1995-12-27 00:00:00
abstract::In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; howe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.062
更新日期:2007-05-29 00:00:00
abstract::Dinucleotide polyphosphates (ApnA) have emerged as signaling molecules in rapidly dividing cells. The presence and role of Ap5A in the heart remain unknown. Here, we report that the myocardium contains abundant amounts of diadenosine 5',5"-P1,P5-pentaphosphate (Ap5A), a member of the ApnA family. Ischemia induced 10-f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00114-8
更新日期:1998-02-27 00:00:00
abstract::TP53 belongs to a small gene family that includes, in mammals, two additional paralogs, TP63 and TP73. The p63 and p73 proteins are structurally and functionally similar to p53 and their activity as transcription factors is regulated by a wide repertoire of shared and unique post-translational modifications and intera...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.06.047
更新日期:2014-08-19 00:00:00