Abstract:
:The predicted Exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient Siberian permafrost sample. The protein bacteriorhodopsin from Exiguobacterium sibiricum (ESR) encoded by this gene was expressed in Escherichia coli membrane. ESR bound all-trans-retinal and displayed an absorbance maximum at 534nm without dark adaptation. The ESR photocycle is characterized by fast formation of an M intermediate and the presence of a significant amount of an O intermediate. Proteoliposomes with ESR incorporated transport protons in an outward direction leading to medium acidification. Proton uptake at the cytoplasmic surface of these organelles precedes proton release and coincides with M decay/O rise of the ESR.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Petrovskaya LE,Lukashev EP,Chupin VV,Sychev SV,Lyukmanova EN,Kryukova EA,Ziganshin RH,Spirina EV,Rivkina EM,Khatypov RA,Erokhina LG,Gilichinsky DA,Shuvalov VA,Kirpichnikov MPdoi
10.1016/j.febslet.2010.09.005subject
Has Abstractpub_date
2010-10-08 00:00:00pages
4193-6issue
19eissn
0014-5793issn
1873-3468pii
S0014-5793(10)00722-2journal_volume
584pub_type
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