Abstract:
:Prochlorococcus marinus SS120 is an ecologically important and biochemically intriguing marine cyanobacterium. In addition to divinyl chlorophylls (DV-Chls) a and b it possesses a particular form of phycoerythrin (PE), but no other phycobilins and therefore no complete phycobilisomes. Here, a spectroscopic characterisation of these DV-Chls and PE is provided. Comparison of fluorescence quantum yields, excited state lifetimes and absorption characteristics indicate similar light-harvesting properties of the DV-Chls as their monovinyl counterparts. PE, which is present only in tiny amounts, was purified and considerably enriched. A phycourobilin to phycoerythrobilin ratio of 3:1 chromophores per (alphabeta) PE monomer is suggested. The in vitro fluorescence lifetime of PE is 1.74 ns. In vivo time-resolved fluorescence measurements with synchrotron radiation were used to investigate the possible role of PE in light-harvesting. The fluorescence decay time for PE is about 550 ps, indicating an unusually slow excitation energy transfer. The decay time slowed to 1 ns after addition of glycerol to cell cultures. The contribution of PE to total light-harvesting capacity was estimated to be about one (alphabeta) PE monomer per 330 DV-Chl b molecules. Thus, the capacity of PE to function primarily as a photosynthetic light-harvesting pigment in P. marinus SS120 is low.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Steglich C,Mullineaux CW,Teuchner K,Hess WR,Lokstein Hdoi
10.1016/s0014-5793(03)00971-2keywords:
subject
Has Abstractpub_date
2003-10-09 00:00:00pages
79-84issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579303009712journal_volume
553pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::HepG2 cells, stably transfected with MDR1 cDNA, encoding the P-glycoprotein multidrug resistance efflux pump, display an altered sphingolipid composition compared to control cells, stably transfected with empty vector. The MDR1 overexpressing cells display a approximately 3-fold increased level of lactosylceramide and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.003
更新日期:2005-06-20 00:00:00
abstract::The cytosolic NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase is ubiquitously expressed in all mouse tissues and cell lines examined. Northern analyses of the RNA indicated that there is an extensive variation in the levels of mRNA in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81354-b
更新日期:1991-12-02 00:00:00
abstract::IGF-I receptors were partially purified from red and white skeletal muscle by lectin-affinity chromatography and the resultant fraction was depleted of insulin receptors by insulin affinity chromatography. Equilibrium binding of 125I-IGF-I to receptor preparations from red and white muscle yielded identical Scatchard ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80093-0
更新日期:1988-07-18 00:00:00
abstract::We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Tran...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.042
更新日期:2006-05-29 00:00:00
abstract::The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate G...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.02.071
更新日期:2006-04-03 00:00:00
abstract::Various vertebrates use ultraviolet (UV) vision for such basic behaviors as mating, foraging, and predation. We have successfully interchanged the color-sensitivities of the mouse UV pigment and the human blue pigment by introducing forward and reverse mutations at five sites. This unveils for the first time the gener...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02269-9
更新日期:2000-12-08 00:00:00
abstract::In this work we have investigated the role of specific peptide sequences for glucose-inactivation of the yeast isocitrate lyase. Thus, different fragments of the ICL1 coding region were fused to the lacZ gene of E. coli to provide a reporter construction. Determinations of beta-galactosidase activities indicated that ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00538-k
更新日期:1995-07-03 00:00:00
abstract::The binding of fibronectin and fibronectin fragments to the enterotoxigenic strain E. coli B34289c was studied. E. coli cells bound to two distinct sites of fibronectin, one being the N-terminal domain, which also contains the binding sites for staphylococci and streptococci, and the other located within the central h...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81238-4
更新日期:1991-09-23 00:00:00
abstract::Melanogenesis is an important biochemical process for the production of skin pigments which protect many animals from the damage of solar radiation. The abnormalities in melanogenesis are associated with albinism, vitiligo, as well as malignant melanoma in humans. In the lower forms of animals viz., insects, the exosk...
journal_title:FEBS letters
pub_type: 更正并重新发布的文章,杂志文章,评审
doi:10.1016/0014-5793(91)81431-7
更新日期:1991-12-16 00:00:00
abstract::Partially purified 2-methyleneglutarate mutase from Clostridium barkeri was separated from 3-methylitaconate delta-isomerase by treatment with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) followed by FPLC on the anion exchange column Mono Q in the presence of the detergent. When purified in the da...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80370-i
更新日期:1991-04-09 00:00:00
abstract::The aim of the present study is to determine the role of intracellular Ca(2+) in VEGF signaling. We demonstrate that reduction in Ca(2+) by chelating compound BAPTA-AM or by IP(3)-endoplasmic reticulum blocker 2-APB selectively inhibited VEGF-induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.05.053
更新日期:2010-07-16 00:00:00
abstract::We show here that the genome of F9 teratocarcinoma cells growing in culture is heavily methylated but undergoes massive demethylation in tumors derived by subcutaneous injection of the cells. This demethylation occurs primarily in single copy gene sequences. As a result imprinted genes acquire their characteristic mon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00994-5
更新日期:1996-10-14 00:00:00
abstract::To identify the novel genes involved in lipid metabolism and lipid droplet formation that may play important roles in Hepatitis C virus (HCV) propagation, we have screened the small interfering RNA library using cell culture derived HCV (HCVcc)-infected cells. We selected and characterized the gene encoding farnesyl-d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.03.043
更新日期:2014-05-02 00:00:00
abstract::The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonol...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00548-0
更新日期:1996-06-17 00:00:00
abstract::The present study provides the first evidence that the abundance of catalytic alpha1-subunit of Na,K-ATPase increases in the course of T cell blast transformation. Immunodepressant cyclosporin A at anti-proliferative doses diminished the induction of alpha1 protein in activated lymphocytes. Furthermore, in competent T...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.03.096
更新日期:2005-05-23 00:00:00
abstract::Decarboxylation of pyruvate by pyruvate decarboxylase (EC 4.1.1.1) was performed in a reaction mixture containing 50% deuterium. The isolated product, acetaldehyde, was investigated directly by 1H NMR and by mass spectrometry after conversion to the 2,4-dinitrophenyl hydrazone. The protium content of 56% at acetaldehy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80238-c
更新日期:1992-03-09 00:00:00
abstract::A cell-free protein expression system was established that provides protein samples of adequate concentration and purity for direct NMR analysis. The Escherichia coli peptidyl-prolyl cis-trans isomerase PpiB was expressed in this system with dual amino acid-selective isotope labeling to identify the NMR signal from th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03048-x
更新日期:2002-07-31 00:00:00
abstract::As a result of an experimental confluence between carcinogenesis and oxidative phosphorylation, we published in 1971 'A Unitary Hypothesis for Carcinogenesis' [(1971) J. Antibiot. Tokyo 24, 405-417]. According to this hypothesis, damaged mitochondria release mitochondrial genetic material which like that from an oncog...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81754-5
更新日期:1989-10-09 00:00:00
abstract::The protein translocation apparatus in Escherichia coli has been studied both genetically and biochemically. In vitro protein translocation systems involving everted membrane vesicles or reconstituted proteoliposomes have significantly contributed to biochemical clarification of the structure, mechanism and energetics...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(94)00317-3
更新日期:1994-06-06 00:00:00
abstract::Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the poly-protein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immuno-precipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80069-7
更新日期:1985-11-25 00:00:00
abstract::Biological activities of five synthetic lipid A analogues (D-glucosamine derivatives) were examined to elucidate the structure required for expression of the biological activities of endotoxin. Proclotting enzyme of horseshoe crab activation, interferon-inducing and tumor necrosis factor-inducing activities were signi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80131-3
更新日期:1984-02-27 00:00:00
abstract::Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved meth...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80318-x
更新日期:1984-04-24 00:00:00
abstract::We studied the effect of acrolein, an alpha,beta-unsaturated aldehyde that causes adduct-modification of lysine, cysteine, and histidine residues, on antithrombin, a key anticoagulant serpin. Intrinsic fluorescence, functionality (anti-FXa and anti-IIa activity), heparin affinity and conformational features of plasma ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.07.062
更新日期:2009-10-06 00:00:00
abstract::Dendritic cells (DC) were enriched from murine spleen by exploring their intermediate density and transient weak adherence. The isolated population contained excellent antigen presenting cells with high surface expression of major histocompatibility complex (MHC) class II determinants thus exhibiting crucial immunofun...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01029-e
更新日期:1995-10-09 00:00:00
abstract::Brain-type creatine kinase B-CK (EC 2.7.3.2) was purified from several chicken tissues, e.g. cardiac muscle, brain, gizzard and retina. Two major monomeric chicken B-CK subunits, designated Bb (basic) and Ba (acidic), which differ in isoelectric point, were separated by chromatofocusing in the presence of 8 M urea on ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80214-4
更新日期:1990-03-26 00:00:00
abstract::When bottromycin A2 was added to an in vitro protein synthesis system carried out by naturally occurring polysomes, it inhibited protein synthesis effectively. Examination of the 3 steps of peptide chain elongation revealed that the binding of aminoacyl-tRNA to the polyribosomes was inhibited by bottromycin A2. In con...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80118-5
更新日期:1983-03-07 00:00:00
abstract::Human red cells suspended in lead-citrate buffers (2.6 microM Pb2+) take up much less Pb than predicted from studies of equilibrium binding of Pb to haemolysates. Pb uptake is increased by ATP depletion, or by loading at 0 degrees C. Tracer studies with 203Pb indicate that the low uptake at 37 degrees C in the presenc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81135-7
更新日期:1984-07-09 00:00:00
abstract::CDK11(p58), a 58kDa protein of the PITSLRE kinase family, plays an important role in cell cycle progression, and is closely related to cell apoptosis. To gain further insight into the function of CDK11(p58), we screened a human fetal liver cDNA library for its interacting proteins using the yeast two-hybrid system. He...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.039
更新日期:2005-07-04 00:00:00
abstract::We have previously generated transgenic (Tg) mice expressing the human apolipoprotein (apo) A-I/C-III/A-IV gene cluster. This expression induced hyperlipidemia but reduced atherosclerotic lesions in genetically modified mice lacking apoE. Atherosclerosis is a multifactorial process and environmental factors such as di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02621-7
更新日期:2001-07-27 00:00:00
abstract::Transport of the precursor for carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells was inhibited by adding monensin or nigericin to the culture medium at a concentration of 0.5 microM, and the enzyme precursor accumulated, mainly in the cytosolic fraction. Accumulated precursor was degrade...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80029-0
更新日期:1984-01-02 00:00:00