Abstract:
:The NK-lysin derived peptide NK-2 is a potent antibacterial, but non-toxic to a human keratinocyte cell line and of low hemolytic activity. Its target selectivity is based upon a strong binding preference to membranes containing anionic phospholipids, which are normally not found on the surface of human cells. Here, we analyzed the interaction of NK-2 with normal human lymphocytes and seven different human cancer cell lines and demonstrate that some of these cells expose negatively charged surface phosphatidylserine (PS), which presumably facilitates killing of the cells by NK-2. This is underlined by the specific intercalation of the peptide into PS-containing liposomes analyzed by fluorescence-resonance energy transfer spectroscopy.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Schröder-Borm H,Bakalova R,Andrä Jdoi
10.1016/j.febslet.2005.09.084keywords:
subject
Has Abstractpub_date
2005-11-07 00:00:00pages
6128-34issue
27eissn
0014-5793issn
1873-3468pii
S0014-5793(05)01208-1journal_volume
579pub_type
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