Abstract:
:Using radiolabeled specific cDNA glutamine synthetase mRNA could be detected by in situ hybridization exclusively within those few perivenous hepatocytes which stained immunocytochemically for glutamine synthetase. This localization of glutamine synthetase mRNA was recently reported by Moorman et al. [(1988) J. Histochem. Cytochem. 36, 751-755]. Biotinylated cDNA was not suitable for mRNA detection because of a very high background staining under the conditions of in situ hybridization. Dot blot and Northern blot analysis of RNA isolated from periportal and perivenous subfractions of hepatocytes also demonstrated the exclusive perivenous localization of two hybridizable glutamine synthetase mRNAs of length 2.8 and 1.6 kilobases. These results indicate that the unique heterogeneity of glutamine synthetase in rat liver parenchyma is controlled at the pretranslational level.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Gebhardt R,Ebert A,Bauer Gdoi
10.1016/0014-5793(88)81037-8subject
Has Abstractpub_date
1988-12-05 00:00:00pages
89-93issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(88)81037-8journal_volume
241pub_type
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