Over-expression of plant 14-3-3 proteins in tobacco: enhancement of the plasmalemma K+ conductance of mesophyll cells.

abstract：:Free and membrane-bound polyribosomes were isolated from the livers of untreated and clofibrate-treated male C57B1/6 mice. The in vitro translation products were investigated in a rabbit reticulocyte cell-free system by immunoprecipitation of cytosolic epoxide hydrolase, catalase and albumin. The soluble forms of epox...

journal_title：FEBS letters

authors： Joste V,Meijer J

更新日期：1989-05-22 00:00:00

abstract：:The number of muscarinic acetylcholine receptors (MAChRs), as detected by binding of [3H]quinuclidinyl benzilate (QNB), was investigated under conditions which promote protein phosphorylation. Incubation of a crude heart membrane preparation in the presence of ATP/Mg2+ reduced MAChR number by 50%. Incubation with poly...

journal_title：FEBS letters

authors： Creazzo TL,Wrenn RW

更新日期：1988-12-19 00:00:00

abstract：:Quiescent fibroblasts derived from human fetal lung can be stimulated to reinitiate DNA synthesis by sequential addition of 3 nM IGF-1 and a low concentration (8 pM) of EGF or by continuous exposure to 10% fetal calf serum or 10 ng/ml PDGF. Bradykinin blocks the IGF-1 and EGF-dependent signals without affecting the re...

journal_title：FEBS letters

authors： Newman EL,Hyldahl L,Larsson O,Engström W,Rees AR

更新日期：1989-07-17 00:00:00

abstract：:The effects of progesterone, dehydroepiandrosterone sulfate and 17beta-estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [3H]glucosamine in the presence of the hormones and then [3H]hyaluronate was isolated from the medium....

journal_title：FEBS letters

authors： Tanaka K,Nakamura T,Takagaki K,Funahashi M,Saito Y,Endo M

更新日期：1997-02-03 00:00:00

abstract：:Proteolytic processing is an important post-translational modification affecting protein activity and stability. In the current study, we investigate the N-terminal cleavage of Trop2, a protein which is overexpressed in many cancers. We demonstrate that Trop2 is cleaved at Arg87 by a transmembrane serine protease, mat...

journal_title：FEBS letters

authors： Kamble PR,Rane S,Breed AA,Joseph S,Mahale SD,Pathak BR

更新日期：2020-10-01 00:00:00

abstract：:A human placenta cDNA expression library was screened for genes inducing flat reversion when transfected into a v-K-ras-transformed NIH3T3 cell line, DT. One such gene was found to encode a Kunitz-type serine protease inhibitor, tissue factor pathway inhibitor-2 (TFPI-2). While the TFPI-2 mRNA can be detected in norma...

journal_title：FEBS letters

authors： Izumi H,Takahashi C,Oh J,Noda M

更新日期：2000-09-08 00:00:00

abstract：:The antibacterial activity of many small, positively charged peptides and proteins is based on pore formation in lipid bilayers. It is here proposed to arise from an electroporation effect. This hypothesis is supported by calculations of the electrostatic potential of NK-lysin associated to a membrane. For a significa...

journal_title：FEBS letters

authors： Miteva M,Andersson M,Karshikoff A,Otting G

更新日期：1999-11-26 00:00:00

abstract：:We have investigated the possible role of selected negatively-charged amino acids of the sodium and chloride-coupled GABA transporter GAT-1 on sodium binding. These residues located adjacent to putative transmembrane domains and which are conserved throughout the large superfamily of neurotransmitter transporters were...

journal_title：FEBS letters

authors： Keshet GI,Bendahan A,Su H,Mager S,Lester HA,Kanner BI

更新日期：1995-08-28 00:00:00

abstract：:The non-selective gastrin/cholecystokinin receptor antagonists proglumide and benzotript inhibit colon carcinoma cell proliferation by binding to the 78 kDa gastrin-binding protein (GBP) (Baldwin, Proc. Natl. Acad. Sci. USA, 91 (1994) 7593-7597). However, although most colon carcinoma cell lines synthesize progastrin,...

journal_title：FEBS letters

authors： Baldwin GS

更新日期：1995-02-06 00:00:00

abstract：:AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants...

journal_title：FEBS letters

authors： Sirpiö S,Holmström M,Battchikova N,Aro EM

更新日期：2009-07-21 00:00:00

abstract：:We have previously shown that topoisomerase I (topo I) antagonist inhibited retrovirus replication. Since tyrphostins, synthetic compounds and protein tyrosine kinases (PTKs) blockers, inhibited topo I activity (manuscript in preparation) we examined their ability to inhibit Moloney murine leukemia virus (Mo-MuLV) rep...

journal_title：FEBS letters

authors： Esther A,Iftach S,Esther P

更新日期：1994-03-14 00:00:00

abstract：:Analogues of S-prenylated cysteine like N-acetyl-S-trans,trans-farnesyl-L-cysteine (AFC) have previously been shown to inhibit the carboxyl methylation of proteins carrying a C-terminal S-prenylated cysteine residue and to block the endotoxin-activated serum-elicited chemotactic response of mouse macrophages. Here, we...

journal_title：FEBS letters

authors： Scheer A,Gierschik P

更新日期：1993-03-15 00:00:00

abstract：:The aim of this investigation was to study the effect of intramitochondrial acyl-CoA on the respiration of rabbit heart mitochondria over the whole range of stationary respiratory rates between States 4 and 3. The creatine phosphokinase system was used for stabilization of extramitochondrial adenine nucleotide concent...

journal_title：FEBS letters

authors： Borutaite V,Mildaziene V,Ivanoviene L,Kholodenko B,Toleikis A,Praskevicius A

更新日期：1989-01-30 00:00:00

abstract：:An open reading frame encoding a putative polypeptide very similar to several lysyl-tRNA synthetases was found 10 nucleotides downstream of Rhizobium meliloti gltX encoding glutamyl-tRNA synthetase. Expression of this gene complemented a mutation in lysS of Escherichia coli and led to the overexpression of a polypepti...

journal_title：FEBS letters

authors： Pelchat M,Gagnon Y,Laberge S,Lapointe J

更新日期：1999-04-16 00:00:00

abstract：:Mutations in the human mtDNA gene encoding subunit III of cytochrome c oxidase (CO) have been reported to cause MELAS and LHON. Poracoccus denitrificans cells expressing substitutions homologous to these MELAS- and LHON-causing mutations had lower growth yield than wild type cells and lower efficiency of proton pumpin...

journal_title：FEBS letters

authors： Mather MW,Rottenberg H

更新日期：1998-08-14 00:00:00

abstract：:The complete amino acid sequence of porcine muscarinic acetylcholine receptor III has been deduced by cloning and sequencing the genomic DNA. The antagonist binding properties of muscarinic acetylcholine receptor III expressed from the cloned DNA in Xenopus oocytes correspond most closely to those of the pharmacologic...

journal_title：FEBS letters

authors： Akiba I,Kubo T,Maeda A,Bujo H,Nakai J,Mishina M,Numa S

更新日期：1988-08-01 00:00:00

abstract：:Electron paramagnetic resonance (EPR) spectroscopy and analysis of the primary structure of the CMP-N-acetylneuraminic acid hydroxylase revealed that this enzyme is the first iron-sulphur protein of the Rieske type to be found in the cytosol of Eukarya. The dithionite-reduced hydroxylase exhibited an EPR signal known ...

journal_title：FEBS letters

authors： Schlenzka W,Shaw L,Kelm S,Schmidt CL,Bill E,Trautwein AX,Lottspeich F,Schauer R

更新日期：1996-05-06 00:00:00

abstract：:In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNA(Met) gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879-2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be ...

journal_title：FEBS letters

authors： Schnare MN,Greenwood SJ,Gray MW

更新日期：1995-03-27 00:00:00

abstract：:Interactions between Trypanosoma cruzi and the extracellular matrix play an important role in cellular invasion. Here we show that T. cruzi increases the levels of thrombospondin-1 (TSP-1) expression in host cells during early infection. Stable RNA interference of host cell TSP-1 knocks down the levels of TSP-1 transc...

journal_title：FEBS letters

authors： Simmons KJ,Nde PN,Kleshchenko YY,Lima MF,Villalta F

更新日期：2006-04-17 00:00:00

abstract：:HepG2 cells, stably transfected with MDR1 cDNA, encoding the P-glycoprotein multidrug resistance efflux pump, display an altered sphingolipid composition compared to control cells, stably transfected with empty vector. The MDR1 overexpressing cells display a approximately 3-fold increased level of lactosylceramide and...

journal_title：FEBS letters

authors： Hummel I,Klappe K,Kok JW

更新日期：2005-06-20 00:00:00

abstract：:In the presence of ADP, the molecular chaperones GroEL and GroES from Escherichia coli not only facilitated the refolding of various proteins, but also prevented their irreversible heat inactivation in vitro. Without nucleotides the refolding reactions were arrested by GroEL. Addition of GroES and ADP to the reaction ...

journal_title：FEBS letters

authors： Kawata Y,Nosaka K,Hongo K,Mizobata T,Nagai J

更新日期：1994-05-30 00:00:00

abstract：:Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in cu...

journal_title：FEBS letters

authors： Shannon MF,Wigley PL,Wells JR

更新日期：1985-07-08 00:00:00

abstract：:In this paper we demonstrate that phage display technology is a suitable system for studying the interaction between the high-affinity receptor for IgE (Fc epsilon RI) and IgE. The alpha subunit extracellular domains of the human receptor were expressed on the surface of filamentous phage M13 fused to the carboxyl-ter...

journal_title：FEBS letters

authors： Scarselli E,Esposito G,Traboni C

更新日期：1993-08-23 00:00:00

abstract：:A new peptide, Tc1, containing only 23 amino acids closely packed by three disulfide bridges was isolated from the Amazonian scorpion Tityus cambridgei. It blocks reversibly the Shaker B K(+)-channels with a K(d) of 65 nM and displaces binding of noxiustoxin to mouse brain synaptosome membranes. It is the shortest kno...

journal_title：FEBS letters

authors： Batista CV,Gómez-Lagunas F,Lucas S,Possani LD

更新日期：2000-12-08 00:00:00

abstract：:Hemin stimulates cAMP production in human peripheral blood mononuclear cells (PBMC). The kinetics are similar to that of hormone-induced cAMP generation, namely a rapid effect followed by a desensitization phase. Several experimental findings suggest that prostaglandins do not mediate this effect. First, macrophage de...

journal_title：FEBS letters

authors： Lander HM,Levine DM,Novogrodsky A

更新日期：1992-06-01 00:00:00

abstract：:Anaerobic biosynthesis of carotenoids in the purple facultative photosynthetic bacterium Rhodobacter sphaeroides was studied using mass spectrometry. We have demonstrated that (18)O from H2(18)O was incorporated into the 1-methoxy group of spheroidene and spheroidenone, the two major carotenoids produced by this bacte...

journal_title：FEBS letters

authors： Yeliseev AA,Kaplan S

更新日期：1997-02-10 00:00:00

abstract：:A new GTP-binding protein serving as the specific substrate of islet-activating protein (IAP), pertussis toxin, was purified from porcine brain membranes as an alpha beta gamma-heterotrimeric structure. The alpha-subunit of the purified protein (alpha 40 beta gamma) had a molecular mass of 40 kDa and differed from tha...

journal_title：FEBS letters

authors： Katada T,Oinuma M,Kusakabe K,Ui M

更新日期：1987-03-23 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ), a ligand-regulated nuclear hormone receptor, plays critical roles in metabolism and adipogenesis. PPARγ ligands such as thiazolidinediones (TZDs) exert insulin sensitizing and anti-inflammatory effects primarily through action on adipocytes, and are thus widely...

journal_title：FEBS letters

authors： Sugii S,Evans RM

更新日期：2011-07-07 00:00:00

abstract：:Using acridine orange as a pH gradient probe, the effects of valinomycin and FCCP on the pH gradient across lysosomal membranes in an ATP-free medium as well as on the rate of the inward ATP-driven proton translocation were investigated. Both lysosome-enriched and highly purified lysosomal preparations from rat liver ...

journal_title：FEBS letters

authors： Dell'Antone P

更新日期：1984-03-12 00:00:00

abstract：:The C-termini of p66 and p51 forms of HIV-1 reverse transcriptase have been engineered to contain a Glu-Glu-Phe sequence recognized by a monoclonal antibody to alpha-tubulin, YL1/2. Mutated RTs were purified in a single step using peptide elution from columns of immobilized YL1/2. The known sequence requirements of th...

journal_title：FEBS letters

authors： Stammers DK,Tisdale M,Court S,Parmar V,Bradley C,Ross CK

更新日期：1991-06-03 00:00:00