Intrinsic uncoupling of cytochrome c oxidase may cause the maternally inherited mitochondrial diseases MELAS and LHON.

abstract：:Amino acid sequencing of a 48/46 kDa glycoprotein from human plantar callus, recognised by antisera raised against the desmosomal cadherins DGII/III, has revealed N-terminal homology to the DNA-derived sequence of human and bovine DGII/III. However, a tryptic fragment has homology only with a bovine clone. We propose ...

journal_title：FEBS letters

authors： King IA,Magee AI,Rees DA,Buxton RS

更新日期：1991-07-29 00:00:00

abstract：:Six chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) Ca(2+) release channels (ryanodine receptors) previously used to identify RyR1 dihydropyridine receptor interactions [Nakai et al. (1998) J. Biol. Chem. 273, 13403] were expressed in HEK293 cells to assess their Ca(2+) dependence in [(3)H]ryanodine b...

journal_title：FEBS letters

authors： Nakai J,Gao L,Xu L,Xin C,Pasek DA,Meissner G

更新日期：1999-10-08 00:00:00

abstract：:We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopu...

journal_title：FEBS letters

authors： Keppler M,Zegrocka O,Strekowski L,Fox KR

更新日期：1999-03-26 00:00:00

abstract：:Fas ligand (FasL) binds Fas (CD95) to induce apoptosis or activate other signaling pathways. In addition, FasL transduces bidirectional or 'reverse signals'. The intracellular domain of FasL contains consensus sequences for phosphorylation and an extended proline rich region, which regulate its surface expression thro...

journal_title：FEBS letters

authors： Thornhill PB,Cohn JB,Drury G,Stanford WL,Bernstein A,Desbarats J

更新日期：2007-09-18 00:00:00

abstract：:Analysis of nascent gamma B-crystallin peptides accumulating during in vitro translation in a rabbit reticulocyte lysate cell-free system was carried out. As a consequence of the irregular distribution of rare codons along the polypeptide chain of gamma B-crystallin, translation of the two-domain protein is a non-unif...

journal_title：FEBS letters

authors： Komar AA,Jaenicke R

更新日期：1995-12-04 00:00:00

abstract：:In order to get a better insight into the function of amino acid residues located in the second transmembrane domain of the cystic fibrosis transmembrane conductance regulator (CFTR) protein, all exon 18 mutations found in cystic fibrosis (CF) patients were characterized at the protein and at the electrophysiological ...

journal_title：FEBS letters

authors： Vankeerberghen A,Wei L,Teng H,Jaspers M,Cassiman JJ,Nilius B,Cuppens H

更新日期：1998-10-16 00:00:00

abstract：:The temperature dependence of the rate constants of the univalent redox steps YzoxSi----YzSi + 1 (i = 0,1,2) and YzoxS3----(YzS4)----YzSo + O2 in the water oxidase was investigated by measuring time resolved absorption changes at 355 nm induced by a laser flash train in dark adapted PS II membrane fragments from spina...

journal_title：FEBS letters

authors： Renger G,Hanssum B

更新日期：1992-03-24 00:00:00

abstract：:Using a radioimmunoassay for the peptide sequence Arg-Phe-NH2 (RFamide), a novel peptide has been purified from acetic acid extracts of the sea anemone Anthopleura elegantissima. This peptide has the structure less than Glu-Gly-Leu-Arg-Trp-NH2, and was named Antho-RWamide II. Antho-RWamide II is a neuropeptide. Its st...

journal_title：FEBS letters

authors： Graff D,Grimmelikhuijzen CJ

更新日期：1988-10-24 00:00:00

abstract：:Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K(+)-efflux and Ca(2+)-influx in Arabidopsis roots. Pre-treatment with either 10μM cAMP or cGMP for 1 or 24h does significantly reduce net K(+)-leakage and Ca(2+)-influx, and in the case of the...

journal_title：FEBS letters

authors： Ordoñez NM,Marondedze C,Thomas L,Pasqualini S,Shabala L,Shabala S,Gehring C

更新日期：2014-03-18 00:00:00

abstract：:When MCF-7 cells were treated with 17 beta-estradiol, dexamethasone, or promegestone at 37 degrees C, the KD of receptors for their cognate ligands was found to decrease as compared to that measured at 2 degrees C. Cell incubation with hormone at 37 degrees C did not affect the Bmax of glucocorticoid and progesterone ...

journal_title：FEBS letters

authors： Rossini GP,Malaguti C

更新日期：1995-12-04 00:00:00

abstract：:STAT3, a transcription factor involved in various physiological and pathological processes, is also present in mitochondria. Mitochondrial STAT3 regulates complex I activity and reactive oxygen species (ROS) production, yet the mechanisms governing its translocation to mitochondria remain poorly understood. In this st...

journal_title：FEBS letters

authors： Mohammed F,Gorla M,Bisoyi V,Tammineni P,Sepuri NBV

更新日期：2020-05-01 00:00:00

abstract：:xxR/KxRxxSxx sequences were phosphorylated with high efficiency by both p70 S6 kinase (p70S6K) and MAPKAP kinase-1. The best substrate for MAPKAP kinase-1 (KKKNRTLSVA) was phosphorylated with a Km of 0.17 microM, and the best substrate for p70S6K (KKRNRTLSVA) with a Km of 1.5 microM. The requirement of both enzymes fo...

journal_title：FEBS letters

authors： Leighton IA,Dalby KN,Caudwell FB,Cohen PT,Cohen P

更新日期：1995-11-20 00:00:00

abstract：:On April 1898 Camillo Golgi communicated to the Medical-Surgical Society of Pavia, the discovery of the "internal reticular apparatus", a novel intracellular organelle which he observed in nerve cells with the silver impregnation he had introduced for the staining of the nervous system. Soon after the discovery it bec...

journal_title：FEBS letters

authors： Mazzarello P,Garbarino C,Calligaro A

更新日期：2009-12-03 00:00:00

abstract：:There are numerous examples of how fundamental research has been required to understand and treat human disease. This article focuses on three human diseases of lipid metabolism in which advancements in understanding and treatment would not have been possible without basic research. Fabry disease is an inherited metab...

journal_title：FEBS letters

authors： Vance DE

更新日期：2006-10-09 00:00:00

abstract：:Cyanomorpholinoadriamycin (1 microM) was reacted in a transcription buffer with DNA of an initiated transcription complex. Subsequent elongation of the initiated complex revealed permanent transcriptional blockages at 16 sites after only 5 min of drug-DNA reaction time. The most dominant sites were immediately prior t...

journal_title：FEBS letters

authors： Cullinane C,Phillips DR

更新日期：1991-11-18 00:00:00

abstract：:The biochemical properties of the p51 subunit of HIV-1 reverse transcriptase (RT) were studied in order to understand its role in the heterodimeric form p66/p51 found in virions. A recombinant form of RT, p51/p51, expressed in yeast, was purified and characterized. The enzyme was affinity labeled using a 5' modified o...

journal_title：FEBS letters

authors： el Dirani-Diab R,Andreola ML,Nevinsky G,Tharaud D,Barr PJ,Litvak S,Tarrago-Litvak L

更新日期：1992-04-13 00:00:00

abstract：:The effects of ribosomal protein L5 on the conformation of 5 S rRNA in the 5 S rRNA-protein L5 complex extracted from rat liver ribosomes have been studied by laser Raman spectroscopy. A comparison of the spectra shows small protein-induced conformational changes in the 5 S rRNA, but most of the base-paired regions ap...

journal_title：FEBS letters

authors： Fabian H,Böhm S,Carius W,Misselwitz R,Welfle H

更新日期：1983-05-08 00:00:00

abstract：:The mutant hen egg white lysozymes Ile55Thr and Asp66His, corresponding to human amyloidogenic mutant lysozymes Ile56Thr and Asp67His, respectively, were secreted in Saccharomyces cerevisiae. The amyloidogenic mutants (I55T and D66H) of hen egg white lysozymes were remarkably less soluble than that of the wild-type pr...

journal_title：FEBS letters

authors： Song Y,Azakami H,Hamasu M,Kato A

更新日期：2001-02-23 00:00:00

abstract：:The bchH gene of Rhodobacter capsulatus has been cloned into an expression strain of Escherichia coli. Following induction of expression of the BchH protein, it was found that the E. coli strain also accumulated porphyrins with the fluorescence properties of protoporphyrin and zinc protoporphyrin. It was also found th...

journal_title：FEBS letters

authors： Hinchigeri SB,Hundle B,Richards WR

更新日期：1997-05-05 00:00:00

abstract：:Mice are widely used for exploring obesity physiology and treatment. However, thermal biology is different between small and large mammals. In this Review, we discuss how the effect of environmental temperature must be understood to ensure applicability of mouse experiments to human obesity. At ambient environmental t...

journal_title：FEBS letters

authors： Reitman ML

更新日期：2018-06-01 00:00:00

abstract：:Previous studies of oxygen-derived free radical generation based on spin-trapping methods have shown a signal formed of six bands (sextet) using electron spin resonance spectrometry (ESR) of coronary effluents collected during post-ischaemic reperfusion of isolated hearts perfused with 5,5-dimethyl-1-pyrroline N-oxide...

journal_title：FEBS letters

authors： Boucher F,Pucheu S,Coudray C,Favier A,de Leiris J

更新日期：1992-05-18 00:00:00

abstract：:Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...

journal_title：FEBS letters

authors： Vines DJ,Warburton MJ

更新日期：1999-01-25 00:00:00

abstract：:The present study reported the isolation and characterization of a novel human secreted protein, named as hPAP21 (human protease-associated domain-containing protein, 21 kDa), encoded by the hypothetical gene chromosome 2 open reading frame 7 (C2orf7) that contains signal peptide in its N-terminus, without transmembra...

journal_title：FEBS letters

authors： Zhou YB,Liu F,Zhu ZD,Zhu H,Zhang X,Wang ZQ,Liu JH,Han ZG

更新日期：2004-10-22 00:00:00

abstract：:Several mRNAs are known to be targeted to dendrites in hippocampal neurons. In this study, we show that brain-derived neurotrophic factor (BDNF) mRNA has two distinct cis-acting dendritic targeting elements in the short 3' untranslated region (UTR): a constitutive element and an activity-dependent one. Moreover, delet...

journal_title：FEBS letters

authors： Oe S,Yoneda Y

更新日期：2010-08-04 00:00:00

abstract：:The primary structure of opsin of the crayfish Procambarus clarkii has been deduced from the cDNA sequence. The opsin is composed of 376 amino acid residues including all the conservative residues characteristic of other members of the rhodopsin family. Comparison of sequences of all known opsins reveals that the majo...

journal_title：FEBS letters

authors： Hariyama T,Ozaki K,Tokunaga F,Tsukahara Y

更新日期：1993-01-11 00:00:00

abstract：:Photon correlation spectroscopy demonstrated for the first time that co-purified meningococcal TbpA+B form a complex in solution. This structure bound hTf and the resultant species underwent partial dissociation after exposure to additional hTf or following prolonged incubation. Purified TbpA and TbpB had similar appa...

journal_title：FEBS letters

authors： Boulton IC,Gorringe AR,Carr RJ,Gorinsky B,Joannou CL,Evans RW

更新日期：1997-09-08 00:00:00

abstract：:A 569 bp probe against the beta-chain of hepatotropin was used to examine expression of RNA for this growth factor in human adult and foetal liver, foetal kidney and pancreas, and rat liver after partial hepatectomy. Low level expression of a 6 kb RNA occurred in human adult and normal rat liver. 70% hepatectomy incre...

journal_title：FEBS letters

authors： Selden C,Jones M,Wade D,Hodgson H

更新日期：1990-09-17 00:00:00

abstract：:Poly(A)-binding protein (PABP) mRNA is subject to autoregulation through a 61 nucleotides long A-rich sequence in its 5' untranslated region (UTR). Here, we show that this mode of regulation is exerted in a cell type-specific manner. Thus, overexpression of PABP in mouse NIH 3T3 fibroblasts represses the translation o...

journal_title：FEBS letters

authors： Hornstein E,Harel H,Levy G,Meyuhas O

更新日期：1999-08-27 00:00:00

abstract：:In the presence of pyrithione, which was used as a Zn2+ ionophore, Zn2+ (10-100 microM) increased phorbol ester binding by intact B-CLL cells in a dose-dependent fashion. Zn pyrithione increased 2-fold the number of phorbol ester receptors in B-cells (0.74 to 1.4 pmol/10(6) cells), neutrophil polymorphs (0.2 to 0.51 p...

journal_title：FEBS letters

authors： Forbes IJ,Zalewski PD,Hurst NP,Giannakis C,Whitehouse MW

更新日期：1989-04-24 00:00:00

abstract：:The NAD-dependent glycerol-3-phosphate dehydrogenase (glycerol-3-phosphate:NAD+ oxidoreductase; EC 1.1.1.8; G3P DHG) was purified 178-fold to homogeneity from Saccharomyces cerevisiae strain H44-3D by affinity- and ion-exchange chromatography. SDS-PAGE indicated that the enzyme had a molecular mass of approximately 42...

journal_title：FEBS letters

authors： Albertyn J,van Tonder A,Prior BA

更新日期：1992-08-17 00:00:00