A new GTP-binding protein in brain tissues serving as the specific substrate of islet-activating protein, pertussis toxin.

abstract：:Activated stellate cells are myofibroblast-like cells associated with the generation of fibrotic scaring in chronically damaged liver. Gene chip analysis was performed on cultured fibrotic stellate cells. Of the 51 human CYP genes known, 13 CYP and 5 CYP reduction-related genes were detected with 4 CYPs (CYP1A1, CYP2E...

journal_title：FEBS letters

authors： Marek CJ,Tucker SJ,Koruth M,Wallace K,Wright MC

更新日期：2007-02-20 00:00:00

abstract：:The 21-residue peptide KLLLLKLLLLKLLLLKLLLLK (KL4) has been synthesized and analyzed regarding its secondary structure and orientation in lipid environments. Fourier transform infrared and circular dichroism spectroscopy shows that the peptide exhibits approximately 80% alpha-helical content both in dodecylphosphochol...

journal_title：FEBS letters

authors： Gustafsson M,Vandenbussche G,Curstedt T,Ruysschaert JM,Johansson J

更新日期：1996-04-15 00:00:00

abstract：:Uncoupling protein 3 (UCP3) may reduce mitochondrial ROS production, and thereby protect against mitochondrial dysfunction in skeletal muscle. UCP3 has been suggested to specifically fulfill this role under high-fat conditions. Here we show that UCP3 knockout mice indeed have elevated mitochondrial ROS production afte...

journal_title：FEBS letters

authors： Nabben M,Hoeks J,Moonen-Kornips E,van Beurden D,Briedé JJ,Hesselink MK,Glatz JF,Schrauwen P

更新日期：2011-12-15 00:00:00

abstract：:Bovine tryptophanyl-tRNA synthetase (TrpRS, E.C.6.1.1.2) is unable to catalyze in vitro formation of Ap4A in contrast to some other aminoacyl-tRNA synthetases. However, in the presence of L-tryptophan, ATP-Mg2+ and ADP the enzyme catalyzes the Ap3A synthesis via adenylate intermediate. Ap3A (not Ap4A) may serve as a s...

journal_title：FEBS letters

authors： Merkulova T,Kovaleva G,Kisselev L

更新日期：1994-08-22 00:00:00

abstract：:It has previously been shown that jasmonic acid affects the ethylene signaling pathway. EIN2 is a central component of ethylene signaling that is downstream of the receptors. EIN2 has previously been shown to be required for ethylene responses. We found that reducing jasmonic acid levels, either mutationally or chemic...

journal_title：FEBS letters

authors： Kim J,Patterson SE,Binder BM

更新日期：2013-01-16 00:00:00

abstract：:The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...

journal_title：FEBS letters

authors： Aspenström P

更新日期：2005-10-10 00:00:00

abstract：:Xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. The affinity to substrate (KM=6-8 muM) and the pH optimum (8.0) of the liver and hepatoma enzymes were the same. The reprogramming of gene...

journal_title：FEBS letters

authors： Prajda N,Weber G

更新日期：1975-11-15 00:00:00

abstract：:Plant receptor-like kinases (RLKs) share their evolutionary origin with animal interleukin-1 receptor-associated kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as 'gatekeeper'. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic c...

journal_title：FEBS letters

authors： Samaddar S,Dutta A,Sinharoy S,Paul A,Bhattacharya A,Saha S,Chien KY,Goshe MB,DasGupta M

更新日期：2013-09-17 00:00:00

abstract：:Both cisplatin and the estrogen receptor (ER) are known to bend DNA. The influence of the bending of sequences by the d(GpG)cisPt adduct binding of ER to estrogen response element (ERE)-like sequences was examined. Three ERE-like oligonucleotides with different affinities for ER and which include a GG in the linker se...

journal_title：FEBS letters

authors： Massaad-Massade L,Massaad C,Legendre F,Bas V,Chottard J,Beaune P,Barouki R

更新日期：2000-01-21 00:00:00

abstract：:The type 1 ribosome-inactivating protein (RIP) saporin 5 isolated from seeds of Saponaria officinalis L. strongly inhibited translation carried out by Vicia sativa L. purified ribosomes. The toxin multidepurinated V. sativa rRNA, which upon treatment with acid aniline releases several RNA fragments including an RNA fr...

journal_title：FEBS letters

authors： Iglesias R,Arias FJ,Rojo MA,Escarmis C,Ferreras JM,Girbés T

更新日期：1993-07-05 00:00:00

abstract：:The HBx protein of hepatitis B virus is involved in deregulation of cell cycle and development of hepatocellular carcinoma. Since c-Myc also plays an important role in cell proliferation and tumor development, we studied its regulation by HBx in a human hepatoma cell line. Co-expression of HBx and c-Myc resulted in in...

journal_title：FEBS letters

authors： Kalra N,Kumar V

更新日期：2006-01-23 00:00:00

abstract：:A mathematical model was developed to estimate flux control coefficients (Co) from titration studies with specific non-competitive inhibitors. In contrast to the normally used graphical determination the model pays regard to the dissociation equilibrium (KD) that exists between inhibitor and its binding sites (Eo) as ...

journal_title：FEBS letters

authors： Gellerich FN,Kunz WS,Bohnensack R

更新日期：1990-11-12 00:00:00

abstract：:Water-soluble amyloid beta-peptides (sA beta), ending at residue 42, precede amyloid plaques in Down's syndrome (DS). Here we report that sA beta consists of the full-length A beta(1-42) and peptides truncated and modified by cyclization of the N-terminal glutamates, A beta[3(pE)-42] and A beta[11(pE)-42]. The A beta[...

journal_title：FEBS letters

authors： Russo C,Saido TC,DeBusk LM,Tabaton M,Gambetti P,Teller JK

更新日期：1997-06-16 00:00:00

abstract：:We have studied the action of an alpha-latrotoxin (alpha-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC12 cells as well as on [14C]GABA release from synaptosomes. ...

journal_title：FEBS letters

authors： Grishin EV,Himmelreich NH,Pluzhnikov KA,Pozdnyakova NG,Storchak LG,Volkova TM,Woll PG

更新日期：1993-12-27 00:00:00

abstract：:Sucrose synthase (SuSy) plays an important role in sucrose degradation and occurs both as a soluble and as a membrane-associated enzyme in higher plants. We show that membrane association can vary in vivo in response to gravistimulation, apparently involving SuSy dephosphorylation, and is a reversible process in vitro...

journal_title：FEBS letters

authors： Winter H,Huber JL,Huber SC

更新日期：1997-12-29 00:00:00

abstract：:SufC is an ATPase component of the SUF machinery, which is involved in the biosynthesis of Fe-S clusters. To gain insight into the function of this protein, we have determined the crystal structure of Escherichia coli SufC at 2.5A resolution. Despite the similarity of the overall structure with ABC-ATPases (nucleotide...

journal_title：FEBS letters

authors： Kitaoka S,Wada K,Hasegawa Y,Minami Y,Fukuyama K,Takahashi Y

更新日期：2006-01-09 00:00:00

abstract：:A chemokine receptor, CXCR4, and its endogenous ligand, stromal cell-derived factor-1 (SDF-1), have been recognized to be involved in the metastasis of several types of cancers. T140 analogs are peptidic CXCR4 antagonists composed of 14 amino acid residues that were previously developed as anti-HIV agents having inhib...

journal_title：FEBS letters

authors： Tamamura H,Hori A,Kanzaki N,Hiramatsu K,Mizumoto M,Nakashima H,Yamamoto N,Otaka A,Fujii N

更新日期：2003-08-28 00:00:00

abstract：:In vertebrate photoreceptors the soluble protein arrestin (45 kDa) is involved in controlling the light dependent activity of receptor proteins such as transducin or the cGMP-phosphodiesterase. Arrestin has further been identified as the retinal-S-antigen which is assumed to cause the autoimmune disease uveitis. In a ...

journal_title：FEBS letters

authors： Glitscher W,Rüppel H

更新日期：1991-05-06 00:00:00

abstract：:To identify genes whose expression is modulated by heregulin-beta1 (HRG), a regulatory polypeptide for mammary epithelial cells, we performed differential display screening of MCF-7 cell mRNA. One cDNA clone upregulated by HRG was identical to human ADP-ribosylation factor 3 (ARF3), a guanine nucleotide-binding protei...

journal_title：FEBS letters

authors： Li F,Mandal M,Mishra SK,Barnes CJ,Kumar R

更新日期：2002-07-31 00:00:00

abstract：:Nuclear magnetic resonance (NMR) spectroscopy was used to determine the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2 (hSCP2), which is a basic protein with 123 residues believed to participate in the intracellular transport of cholesterol and various oth...

journal_title：FEBS letters

authors： Szyperski T,Scheek S,Johansson J,Assmann G,Seedorf U,Wüthrich K

更新日期：1993-11-29 00:00:00

abstract：:It has previously been suggested that the mammary cell could produce prolactin (PRL). This hypothesis was investigated by incubation with [35S]methionine-cysteine followed by SDS-PAGE, immunoblotting and autoradiography of immunoprecipitated PRL, and by electron microscopic analysis after incubation without or with cy...

journal_title：FEBS letters

authors： Lkhider M,Delpal S,Le Provost F,Ollivier-Bousquet M

更新日期：1997-01-20 00:00:00

abstract：:The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited...

journal_title：FEBS letters

authors： Robinson IM,Burgoyne RD

更新日期：1991-09-09 00:00:00

abstract：:Patients suffering from Refsum disease have a defect in the alpha-oxidation pathway which results in the accumulation of phytanic acid in plasma and tissues. Our previous studies have shown that phytanic acid is also a substrate for the omega-oxidation pathway. With the use of specific inhibitors we now show that memb...

journal_title：FEBS letters

authors： Komen JC,Wanders RJ

更新日期：2006-07-10 00:00:00

abstract：:We have examined the formation of DNA triple helices between the oligonucleotides T8XT8 (X = A,C,G,T) and DNA fragments containing the target sequences A8XA8.T8YT8 (X = T,C,G; Y = A,G,C), by DNase I footprinting. We find that A8GA8.T8CT8 yields a footprint with T8CT8 and shows a weaker interaction with T17 and T8GT8. ...

journal_title：FEBS letters

authors： Chandler SP,Fox KR

更新日期：1993-10-11 00:00:00

abstract：:Matrix metalloproteinase-13 (MMP-13) plays a critical role in parathyroid hormone (PTH)-induced bone resorption. PTH acts via protein kinase A (PKA) to phosphorylate and stimulate the transactivation of Runx2 for MMP-13 promoter activation. We show here that PTH stimulated Runx2 phosphorylation in rat osteoblastic cel...

journal_title：FEBS letters

authors： Selvamurugan N,Shimizu E,Lee M,Liu T,Li H,Partridge NC

更新日期：2009-04-02 00:00:00

abstract：:Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

journal_title：FEBS letters

authors： Döbbeling U,Berchtold MW

更新日期：1996-08-05 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ), a ligand-regulated nuclear hormone receptor, plays critical roles in metabolism and adipogenesis. PPARγ ligands such as thiazolidinediones (TZDs) exert insulin sensitizing and anti-inflammatory effects primarily through action on adipocytes, and are thus widely...

journal_title：FEBS letters

authors： Sugii S,Evans RM

更新日期：2011-07-07 00:00:00

abstract：:The effects of various local anaesthetics (LAs) on ryanodine binding of the sheep brain ryanodine receptor were tested. Tetracaine and dibucaine inhibit the binding with half-maximal inhibition (CI50) of 0.12 mM and 0.7 mM, respectively. Lidocaine and its analog QX-314, on the other hand, stimulate the binding up to 3...

journal_title：FEBS letters

authors： Martin C,Ashley R,Shoshan-Barmatz V

更新日期：1993-08-09 00:00:00

abstract：:Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory p...

journal_title：FEBS letters

authors： Kairane C,Zilmer M,Mutt V,Sillard R

更新日期：1994-05-23 00:00:00

abstract：:To gain an understanding of the mechanism by which the hydroxyl free radical can arise in superoxide generating systems and learn how different chelaters of iron can inhibit this reaction, a pulse radiolysis kinetic study of the reaction of O;- with Fe(III)EDTA, Fe(III)HEDTA and Fe(III)DETAPAC (or DTPA)was undertaken....

journal_title：FEBS letters

authors： Buettner GR,Doherty TP,Patterson LK

更新日期：1983-07-11 00:00:00