The 21-residue surfactant peptide (LysLeu4)4Lys(KL4) is a transmembrane alpha-helix with a mixed nonpolar/polar surface.


:The 21-residue peptide KLLLLKLLLLKLLLLKLLLLK (KL4) has been synthesized and analyzed regarding its secondary structure and orientation in lipid environments. Fourier transform infrared and circular dichroism spectroscopy shows that the peptide exhibits approximately 80% alpha-helical content both in dodecylphosphocholine micelles and in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/phosphatidylglycerol (PG) 7:3 (w/w) bilayers. The positively charged lysine residues are evenly distributed over the entire, otherwise nonpolar, circumference of the helix. This is in sharp contrast to the uneven distribution of polar and nonpolar residues in amphipathic helices. Fourier transform infrared spectroscopy of the peptide inserted in DPPC/PG bilayers shows that the helical axis is oriented parallel to the lipid acyl chains. These data do not support a previous hypothesis that the KL4 peptide interacts with peripheral parts of a phospholipid monolayer and mimics the pulmonary surfactant protein SP-B, which is composed of several amphipathic alpha-helices. KL4 accelerates the spreading of phospholipid mixtures at an air/water interface but does so less efficiently than other transmembranous helical polypeptides studied.




FEBS letters


Gustafsson M,Vandenbussche G,Curstedt T,Ruysschaert JM,Johansson J




Has Abstract


1996-04-15 00:00:00














  • Relative contribution of Ca2+-dependent and Ca2+-independent mechanisms to the regulation of insulin secretion by glucose.

    abstract::Although insulin secretion is usually regarded as a Ca2+-dependent mechanism, recent studies have suggested the existence of a Ca2+-independent pathway of regulation by glucose. Here, mouse islets were used to compare the contribution of Ca2+-dependent and -independent pathways. Glucose increased insulin release in a ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sato Y,Nenquin M,Henquin JC

    更新日期:1998-01-09 00:00:00

  • Synthesis of a hydrolase for the membrane-form variant surface glycoprotein is repressed during transformation of Trypanosoma brucei.

    abstract::A membrane-bound phospholipase C-like hydrolase present in lysates of bloodstream forms of Trypanosoma brucei rapidly converts the membrane form of the variant surface protein to the soluble form and 1,2-dimyristoylglycerol [(1985) M.A.J. Ferguson et al. J. Biol. Chem., 260, 4963-4968]. The hydrolase is inhibited by p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bülow R,Overath P

    更新日期:1985-07-22 00:00:00

  • The kinetics of V-J joining throughout 3.5 megabases of the mouse Ig kappa locus fit a constrained diffusion model of nuclear organization.

    abstract::To gain insight into the nuclear organization of the mouse Ig kappa locus and how it may relate to the formation of synapses during recombination, we have studied the kinetics of rearrangement of different V kappa gene families to J kappa gene segments in the pre-B cell line, 103bcl2. Remarkably, V kappa gene families...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Li S,Garrard WT

    更新日期:2003-02-11 00:00:00

  • New insights into human primordial germ cells and early embryonic development from single-cell analysis.

    abstract::Human preimplantation developmental studies are difficult to accomplish due to associated ethical and moral issues. Preimplantation cells are rare and exist only in transient cell states. From a single cell, it is very challenging to analyse the origination of the heterogeneity and complexity inherent to the human bod...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Otte J,Wruck W,Adjaye J

    更新日期:2017-08-01 00:00:00

  • Delineation of the TRAK binding regions of the kinesin-1 motor proteins.

    abstract::Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin-1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin-1 ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Randall TS,Moores C,Stephenson FA

    更新日期:2013-11-29 00:00:00

  • Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae.

    abstract::Rho proteins are down-regulated in vivo by specific GTPase activating proteins (RhoGAP). We have functionally studied three Saccharomyces cerevisiae putative RhoGAP. By first identifying Rho partners with a systematic two-hybrid approach and then using an in vitro assay, we have demonstrated that the Bag7 protein stim...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roumanie O,Weinachter C,Larrieu I,Crouzet M,Doignon F

    更新日期:2001-10-05 00:00:00

  • Structural organization of the bovine cathelicidin gene family and identification of a novel member.

    abstract::Cathelicidins are a group of myeloid antimicrobial peptide precursors found in a variety of mammalian species. Transcripts of this family show a highly conserved 5' region corresponding to the 5' untranslated region, signal peptide and propiece, and diverse 3' regions encoding structurally varied C-terminal sequences ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Scocchi M,Wang S,Zanetti M

    更新日期:1997-11-17 00:00:00

  • Purification and functional characterization of beta-adrenergic receptor kinase expressed in insect cells.

    abstract::The beta-adrenergic receptor kinase mediates agonist-dependent phosphorylation of beta-adrenergic receptors, which is thought to represent the first step of homologous desensitization. We have expressed bovine and human beta ARK1 in Sf9 cells and purified them to apparent homogeneity in milligram quantities. The Km-va...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Söhlemann P,Hekman M,Buchen C,Elce JS,Lohse MJ

    更新日期:1993-06-07 00:00:00

  • EGF induces efficient Cx43 gap junction endocytosis in mouse embryonic stem cell colonies via phosphorylation of Ser262, Ser279/282, and Ser368.

    abstract::Gap junctions (GJs) traverse apposing membranes of neighboring cells to mediate intercellular communication by passive diffusion of signaling molecules. We have shown previously that cells endocytose GJs utilizing the clathrin machinery. Endocytosis generates cytoplasmic double-membrane vesicles termed annular gap jun...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fong JT,Nimlamool W,Falk MM

    更新日期:2014-03-03 00:00:00

  • Nature of respiratory stimulation in hyperthyroidism: the redox behaviour of cytochrome c.

    abstract::Hyperthyroid mitochondria show an increased Km and Vmax in the high affinity phase of cytochrome oxidase kinetics. During inhibitor titrations, cytochrome c shows a different redox behaviour in hyperthyroid with respect to protonophore-treated euthyroid mitochondria. The observations are discussed in terms of a differ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schmehl I,Luvisetto S,Canton M,Gennari F,Azzone GF

    更新日期:1995-11-20 00:00:00

  • Autophosphorylation of the two C-terminal tyrosine residues Tyr1316 and Tyr1322 modulates the activity of the insulin receptor kinase in vitro.

    abstract::Previously, several studies have demonstrated that autophosphorylation of the C-terminal tyrosine residues (Tyr1316 and Tyr1322) affects the signaling properties of the insulin receptor in vivo. To assess the biochemical consequences of the C-terminal phosphorylation in vitro, we have constructed, purified and charact...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tennagels N,Bergschneider E,Al-Hasani H,Klein HW

    更新日期:2000-08-11 00:00:00

  • Molecular cloning of the canine angiotensin II receptor. An AT1-like receptor with reduced affinity for DuP753.

    abstract::Canine aortic strip studies revealed insensitivity of angiotensin II (AII)-induced aortic contraction to inhibition by the non-peptide antagonist DuP753 (pKB = 6.7 +/- 0.1). In order to determine the origin of this phenomenon we cloned the canine homologue of the AT1 AII receptor. Expression of this cDNA in COS-7 cell...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Burns L,Clark KL,Bradley J,Robertson MJ,Clark AJ

    更新日期:1994-04-25 00:00:00

  • Substrate properties of C'-methyl UTP derivatives in T7 RNA polymerase reactions. Evidence for N-type NTP conformation.

    abstract::The number of synthetic UTP analogues containing methyl groups in different positions of the ribose moiety were tested as substrates for T7 RNA polymerase (T7 RNAP). Two of these compounds (containing substituents in the 5' position) were shown to be weak substrates of T7 RNAP. 3'Me-UTP was neither substrate nor inhib...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tunitskaya VL,Rusakova EE,Padyukova NSh,Ermolinsky BS,Chernyi AA,Kochetkov SN,Lysov YuP,Mikhailov SN

    更新日期:1997-01-06 00:00:00

  • A bifunctional Galphai/Galphas modulatory peptide that attenuates adenylyl cyclase activity.

    abstract::Signaling via G-protein coupled receptors is initiated by receptor-catalyzed nucleotide exchange on Galpha subunits normally bound to GDP and Gbetagamma. Activated Galpha . GTP then regulates effectors such as adenylyl cyclase. Except for Gbetagamma, no known regulators bind the adenylyl cyclase-stimulatory subunit Ga...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Johnston CA,Ramer JK,Blaesius R,Fredericks Z,Watts VJ,Siderovski DP

    更新日期:2005-10-24 00:00:00

  • Zwitterionic amphiphiles that raise the bilayer to hexagonal phase transition temperature inhibit protein kinase C. The exception that proves the rule.

    abstract::We synthesized the zwitterionic amphiphile cholesterylphosphorylethylpyridinium. This substance activated protein kinase C (PKC) in a micelle-based assay, but in a vesicle assay it was inhibitory. An analog of this compound, in which the pyridine ring is saturated and the nitrogen methylated, showed similar behaviour ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Epand RM,Stafford A,Wang J,Epand RF

    更新日期:1992-06-15 00:00:00

  • Mutations at residues Tyr771 and Tyr783 of phospholipase C-gamma1 have different effects on cell actin-cytoskeleton organization and cell proliferation in CCL-39 cells.

    abstract::Currently a central question that remains unresolved is the potential importance of phospholipase C (PLC)-gamma1 in cell mitogenesis. In this study, we introduced wild-type PLC-gamma1 plasmid and mutants Y771F and Y783F into CCL-39 fibroblasts and investigated their effect on host cell functions. To our surprise, Y771...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pei ZD,Williamson JR

    更新日期:1998-02-13 00:00:00

  • Sialic acid is an essential moiety of mucin as a hydroxyl radical scavenger.

    abstract::In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; howe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ogasawara Y,Namai T,Yoshino F,Lee MC,Ishii K

    更新日期:2007-05-29 00:00:00

  • Function of a separate NH3-pore in Aquaporin TIP2;2 from wheat.

    abstract::Functional analysis of heterologously expressed TaTIP2;2 by means of stopped-flow spectrometric studies provide evidence for water and ammonia conductivity. A series of experiments under increasing pH indicate that the gaseous NH(3), rather than the ammonium ion NH(4)(+) was transported. Results from inhibitor studies...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bertl A,Kaldenhoff R

    更新日期:2007-11-27 00:00:00

  • Regulation of bioactive beta-endorphin processing in rat pars intermedia.

    abstract::Acid extracts of rat pituitary neuro-intermediate lobes have been shown by ion-exchange chromatography and radio-immunoassay to contain predominantly the inactive derivatives of beta-endorphin, alpha, N-acetyl beta-endorphin 1-27 and alpha, N-acetyl beta-endorphin 1-26; the biologically active form, beta-endorphin 1-3...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ham J,Smyth DG

    更新日期:1984-10-01 00:00:00

  • Searching for the mechanism of signalling by plant photoreceptor cryptochrome.

    abstract::Even though the plant photoreceptors cryptochromes were discovered more than 20 years ago, the mechanism through which they transduce light signals to their partner molecules such as COP1 (Constitutive Photomorphogenic 1) or SPA1 (Suppressor of Phytochrome A) still remains to be established. We propose that a negative...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Müller P,Bouly JP

    更新日期:2015-01-16 00:00:00

  • Biotin synthase mechanism: on the origin of sulphur.

    abstract::Biotin synthase catalyses the last step of the biosynthesis of biotin in microorganisms and plants. The active protein isolated from Bacillus sphaericus and Escherichia coli contains an iron-sulphur (FeS) cluster. The native enzymes were depleted of their iron and inorganic sulphide and the resulting apoenzymes were c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bui BT,Florentin D,Fournier F,Ploux O,Méjean A,Marquet A

    更新日期:1998-11-27 00:00:00

  • Induction of volatile biosynthesis in the lima bean (Phaseolus lunatus) by leucine- and isoleucine conjugates of 1-oxo- and 1-hydroxyindan-4-carboxylic acid: evidence for amino acid conjugates of jasmonic acid as intermediates in the octadecanoid signalli

    abstract::One of the most intriguing plant defense reactions against herbivores is the emission of volatiles as potentially attractive signals for the natural enemies of the attacking species. Like many other low and high molecular weight chemical defenses, volatile production is under the control of the octadecanoid signalling...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Krumm T,Bandemer K,Boland W

    更新日期:1995-12-27 00:00:00

  • Structure des parois cellulaires des nocardia. I-isolement et composition des parois de Nocardia kirovani.

    abstract::Nocardia kirovani cell walls possess a lipidic fraction, a lipolysaccharidic fraction and a peptidoglycan. The lipidic part is composed of glycerides, C(16) and C(18) free fatty acids, nocardic acids and of a carotenoid pigment. The lipopolysaccharidic part is composed of an arabinogalactan esterified by nocardic acid...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guinand M,Vacheron MJ,Michel G

    更新日期:1970-01-15 00:00:00

  • Characterization of cDNA encoding the mouse hepatic triglyceride lipase and expression by in vitro translation.

    abstract::A cDNA coding for the mouse hepatic triglyceride lipase has been isolated from a mouse liver cDNA library. The nucleotide sequence of the cDNA shows an open reading frame encoding a polypeptide of 510 amino acids that is 91.5% and 86% homologous to rat and human hepatic lipase, respectively. The most drastic protein s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chang SF,Netter HJ,Will H

    更新日期:1991-09-02 00:00:00

  • The interaction of fructose 2,6-bisphosphate with an allosteric site of rat liver fructose 1,6-bisphosphatase.

    abstract::Rat liver fructose 1,6-bisphosphatase can be protected against partial inactivation by N-ethylmaleimide by low concentrations of fructose 2,6-bisphosphate or high concentrations of fructose 1,6-bisphosphate. The partially inactivated enzyme has a much reduced sensitivity to high substrate inhibition and has lost the s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Meek DW,Nimmo HG

    更新日期:1983-08-22 00:00:00

  • Role of the C-terminal domain of Bax and Bcl-XL in their localization and function in yeast cells.

    abstract::It has been suggested that the C-terminal domain of Bcl-2 family members may contain a signal anchor sequence that targets these proteins to the mitochondrial outer membrane. We have investigated the consequence of deleting this domain upon cytochrome c release in yeast strains that coexpress truncated forms of Bax (i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Priault M,Camougrand N,Chaudhuri B,Manon S

    更新日期:1999-01-25 00:00:00

  • High concentrations of low density lipoprotein decrease basement membrane-associated heparan sulfate proteoglycan in cultured endothelial cells.

    abstract::The effect of increasing low density lipoprotein (LDL) concentrations on the synthesis of basement membrane components was investigated in proliferating porcine aortic endothelial cells (PAEC) in culture. Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin were determined by enzyme immunoa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Olgemöller B,Schleicher ED,Schwaabe S,Guretzki HJ,Gerbitz KD

    更新日期:1990-05-07 00:00:00

  • Protein NMR resonance assignment by isotropic mixing experiments on random fractionally deuterated samples.

    abstract::The 108-residue protein E. coli thioredoxin has been uniformly enriched to 50% with deuterium at all carbon-bound hydrogen positions. Isotropic mixing (i.e. TOCSY) experiments have been conducted for both the deuterated and natural-abundance samples. Using a 54 ms mixing time correlation peaks can be seen for all four...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: LeMaster DM

    更新日期:1988-06-20 00:00:00

  • Chaos in DNA partition during the last mitoses of the proliferative life-span of human fibroblasts.

    abstract::We have followed with autoradiography the course of DNA synthesis and with cytofluorometry the partition of DNA during mitosis, through the different phases of the life-span of human fibroblasts. Towards the end of the proliferative potential of the cell population, DNA synthesis was disturbed during the late part of ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Macieira-Coelho A

    更新日期:1995-01-23 00:00:00

  • Primary structure of crayfish visual pigment deduced from cDNA.

    abstract::The primary structure of opsin of the crayfish Procambarus clarkii has been deduced from the cDNA sequence. The opsin is composed of 376 amino acid residues including all the conservative residues characteristic of other members of the rhodopsin family. Comparison of sequences of all known opsins reveals that the majo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hariyama T,Ozaki K,Tokunaga F,Tsukahara Y

    更新日期:1993-01-11 00:00:00