Rat prolactin synthesis by lactating mammary epithelial cells.

Abstract:

:It has previously been suggested that the mammary cell could produce prolactin (PRL). This hypothesis was investigated by incubation with [35S]methionine-cysteine followed by SDS-PAGE, immunoblotting and autoradiography of immunoprecipitated PRL, and by electron microscopic analysis after incubation without or with cycloheximide. Immunoreactive 14-, 23-, 25-, 32- and 36-kDa PRL forms were radioactive. By two-dimensional electrophoresis analysis, immunoreactive and radioactive spots, of about 25 kDa and high molecular weight, were also detected. After incubation of mammary epithelial cells with cycloheximide, immunogold electron microscopy showed a drastic decrease of labelling in organelles involved in synthesis and secretion, compared to those incubated in control medium. These results make it possible to conclude that lactating mammary tissue is able to synthesize PRL.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Lkhider M,Delpal S,Le Provost F,Ollivier-Bousquet M

doi

10.1016/s0014-5793(96)01450-0

subject

Has Abstract

pub_date

1997-01-20 00:00:00

pages

117-22

issue

2-3

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(96)01450-0

journal_volume

401

pub_type

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