Abstract:
:It has previously been suggested that the mammary cell could produce prolactin (PRL). This hypothesis was investigated by incubation with [35S]methionine-cysteine followed by SDS-PAGE, immunoblotting and autoradiography of immunoprecipitated PRL, and by electron microscopic analysis after incubation without or with cycloheximide. Immunoreactive 14-, 23-, 25-, 32- and 36-kDa PRL forms were radioactive. By two-dimensional electrophoresis analysis, immunoreactive and radioactive spots, of about 25 kDa and high molecular weight, were also detected. After incubation of mammary epithelial cells with cycloheximide, immunogold electron microscopy showed a drastic decrease of labelling in organelles involved in synthesis and secretion, compared to those incubated in control medium. These results make it possible to conclude that lactating mammary tissue is able to synthesize PRL.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lkhider M,Delpal S,Le Provost F,Ollivier-Bousquet Mdoi
10.1016/s0014-5793(96)01450-0subject
Has Abstractpub_date
1997-01-20 00:00:00pages
117-22issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(96)01450-0journal_volume
401pub_type
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