Abstract:
:Sucrose synthase (SuSy) plays an important role in sucrose degradation and occurs both as a soluble and as a membrane-associated enzyme in higher plants. We show that membrane association can vary in vivo in response to gravistimulation, apparently involving SuSy dephosphorylation, and is a reversible process in vitro. Phosphorylation of SuSy has little effect on its activity but decreases its surface hydrophobicity as reported with the fluorescent probe bis-ANS. We postulate that phosphorylation of SuSy (and perhaps other membrane proteins) is involved in the release of the membrane-bound enzyme in part as a result of decreased surface hydrophobicity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Winter H,Huber JL,Huber SCdoi
10.1016/s0014-5793(97)01506-8subject
Has Abstractpub_date
1997-12-29 00:00:00pages
151-5issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01506-8journal_volume
420pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::By applying the 'recognition mask' strategy to 300 mammalian sequences containing NotI sites we demonstrated that 5' ends of genes are highly enriched in NotI sites. A NotI linking clone NL2-252 (D3S1678) containing transferrin receptor (TFRC) gene was used as an initial point for chromosomal jumping. One of the jumpi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01449-x
更新日期:1997-12-15 00:00:00
abstract::Stroke is characterized by massive inflammation in areas surrounding the injury that magnifies damage to the brain. The liver X receptors (LXRs) are nuclear receptors that regulate cholesterol, lipid, and glucose metabolism. Synthetic LXR agonists have potent anti-inflammatory properties in a variety of settings, incl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.035
更新日期:2008-10-15 00:00:00
abstract::Cathepsin L is a ubiquitously expressed papain-like cysteine protease involved in the endosomal degradation of proteins and has numerous roles in physiological and pathological processes, such as arthritis, osteoporosis, and cancer. Insight into the specificity of cathepsin L is important for elucidating its physiolog...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12140
更新日期:2016-04-01 00:00:00
abstract::In the ameboid eukaryote Dictyostelium discoideum, chemotactic stimulation by cAMP induces an increase of intracellular cGMP and subsequently the phosphorylation of myosin heavy chain II. Resistance to high osmotic stress also requires transient increases of intracellular cGMP and phosphorylation of myosin heavy chain...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00183-5
更新日期:1998-03-13 00:00:00
abstract::The homeodomain protein, Cdx2, has been implicated in the transcriptional regulation of genes expressed in the small intestine. In vitro studies of the carbonic anhydrase 1 (CA1) colon promoter implied that Cdx2 may also play a role in the regulation of colon-specific gene expression. The current work follows up this ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00103-3
更新日期:1998-02-20 00:00:00
abstract::The 36 kDa fragment of actin molecule obtained with the protease from E. coli A2 strain [(1988) FEBS Lett. 228, 172] was shown to begin with Val-43 and retain the COOH-terminal amino acid residues of the parent molecule. The E. coli protease split actin preserves the NH2-terminal part of the polypeptide chain as well ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80247-z
更新日期:1991-02-11 00:00:00
abstract::The minimal functional enzyme unit of Na,K-ATPase consists of an alpha-beta complex. The alpha-subunit bears all functional domains of the enzyme and so far a regulatory role for the beta-subunit in the catalytic cycle has not been established. On the other hand, increasing experimental evidence suggests that the beta...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(91)80801-9
更新日期:1991-07-22 00:00:00
abstract::A monoclonal antibody (mAb) has been produced which reacts with human mitofilin, a mitochondrial inner membrane protein. This mAb immunocaptures its target protein in association with six other proteins, metaxins 1 and 2, SAM50, CHCHD3, CHCHD6 and DnaJC11, respectively. The first three are outer membrane proteins, CHC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.06.052
更新日期:2007-07-24 00:00:00
abstract::Phalloidin and fluorescently labeled phalloidin analogs are established reagents to stabilize and mark actin filaments for the investigation of acto-myosin interactions. In the present study, we employed transient and steady-state kinetic measurements as well as in vitro motility assays to show that phalloidin perturb...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.01.042
更新日期:2011-03-09 00:00:00
abstract::We have purified a 38 kDa protein from bovine brain which is cross-reactive with an affinity purified antibody against the 35 kDa phosphatidylinositol transfer protein from the same source. Controlled trypsinization of the 38 kDa protein yielded an immunoreactive protein of 35 kDa which displayed a 6-fold increase in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80523-l
更新日期:1990-12-10 00:00:00
abstract::Structure/function relationships in troponin C are studied with vertebrate fast-twitch fibers by exchanging the skeletal troponin C with two bacterially synthesized recombinant proteins designed by site-directed mutagenesis of cardiac troponin C. One mutant (CBM1) contained an additional active site, by deleting Val-2...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80420-x
更新日期:1989-05-08 00:00:00
abstract::In order to study the function of the bovine MAP4 microtubule-binding domain (the assembly-promoting (AP) sequence region), a fragment corresponding to the AP sequence region was prepared using an Escherichia coli expression system. When the fragment was mixed with purified tubulin at 37 degrees C, the fragment caused...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01342-2
更新日期:1997-11-24 00:00:00
abstract::Transcriptional responses to growth in high environmental calcium concentrations were characterized and compared between wild-type and mutant Arabidopsis plants containing a knockout mutation in the gene encoding a cyclic nucleotide-gated channel (CNGC2). We show that the transcriptional profile of cngc2 plants grown ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.02.043
更新日期:2008-03-19 00:00:00
abstract::(7-methoxycoumarin-4-yl)Acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L- 2,3-diaminopropionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) has been synthesised as a fluorogenic substrate for the matrix metalloproteinases. The highly fluorescent 7-methoxycoumarin group is efficiently quenched by energy transfer t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80300-6
更新日期:1992-01-27 00:00:00
abstract::To identify suitable promoters for expressing exogenous genes in arthritic joints, the constitutive, simian virus 40 (SV 40) and IL-1 or metal inducible, human stromelysin and metallothionein (MT) gene promoters were tested for their activity in chondrocytes and synovial fibroblasts. Transient transfection with plasmi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01037-x
更新日期:1996-10-21 00:00:00
abstract::The tetraspanins associate with various surface molecules and with each other to build a network of molecular interactions, the tetraspanin web. The interaction of tetraspanins with each other seems to be central for the assembly of the tetraspanin web. All tetraspanins studied, CD9, CD37, CD53, CD63, CD81, CD82 and C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02522-x
更新日期:2002-04-10 00:00:00
abstract::Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01028-9
更新日期:1996-10-21 00:00:00
abstract::Neuronal activity is often associated with changes in gene expression. By a two-dimensional cDNA-display system, restriction landmark cDNA scanning, we identified a novel gene whose expression in the hippocampus was up-regulated by kainate stimulation. The mRNA expression was detected only in brain and up-regulated by...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00497-9
更新日期:1998-05-22 00:00:00
abstract::The CLN3 gene encodes an integral membrane protein of unknown function. Mutations in CLN3 can cause juvenile neuronal ceroid lipofuscinosis, or Batten disease, an inherited neurodegenerative lysosomal storage disease affecting children. Here, we report a topological study of the CLN3 protein using bioinformatic approa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.02.049
更新日期:2008-04-02 00:00:00
abstract::We studied the effect of high-fat diet on the expression and activation of the three caveolins in rat skeletal muscle and their association with the insulin signalling cascade. Initial response was characterized by increased signalling through Cav-1 and Cav-3 phosphorylation, suggesting that both participate in an ini...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.09.016
更新日期:2009-10-06 00:00:00
abstract::Proteins containing the caspase recruiting domain (CARD) have emerged as critical regulators of different signal transduction pathways, including those controlling apoptosis and activation of necrosis factor (NF)-kappaB transcription factor. TUCAN/CARDINAL is a recently identified CARD-containing protein involved in r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02869-7
更新日期:2002-06-19 00:00:00
abstract::The type 1 ribosome-inactivating protein (RIP) saporin 5 isolated from seeds of Saponaria officinalis L. strongly inhibited translation carried out by Vicia sativa L. purified ribosomes. The toxin multidepurinated V. sativa rRNA, which upon treatment with acid aniline releases several RNA fragments including an RNA fr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81091-d
更新日期:1993-07-05 00:00:00
abstract::It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric trans...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00097-x
更新日期:1996-02-26 00:00:00
abstract::Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00269-0
更新日期:1999-03-19 00:00:00
abstract::The release of a GPI-linked oncofetal protein (PLAP) from HEp-2 cells has been studied as an indicator of endogenous PI-specific PLC activation. A non-hydrolysable GTP analogue, EGF and insulin all produced a dose-dependent release of PLAP from these cells. This indicates that an increase in PLC activity following eit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80927-b
更新日期:1990-07-16 00:00:00
abstract::Muscarinic acetylcholine receptors purified from porcine atrium were phosphorylated, depending on the presence of agonists, by a protein kinase partially purified from porcine brain, which had similar properties to the beta-adrenergic receptor kinase. GTP-binding regulatory proteins (Go) had dual effects on the phosph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80968-o
更新日期:1990-07-30 00:00:00
abstract::All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-Jkappa. mNotch2, mNotch3 and int3 (= mNotch4) were shown to interact with RBP-Jkappa by the GST-fusion pull down assay and dominant negative competition with Epstein Barr ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01046-0
更新日期:1996-10-21 00:00:00
abstract::The nuclear magnetic resonance solution structure of alpha-conotoxin SI has been determined at pH 4.2. The 36 lowest energy structures show that alpha-conotoxin SI exists in a single major solution conformation and is stabilized by six hydrogen bonds. Comparisons are made between the SI solution structure and the solu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01724-5
更新日期:2000-07-07 00:00:00
abstract::The enantiomers of U50,488, ligands highly selective for kappa-opioid receptors, have been prepared by a refined procedure and their optical purity demonstrated. The absolute configuration of (+)-trans-2-pyrrolidinyl-N-methylcyclohexylamine, a chemically versatile intermediate for synthesis of analogs of kappa-opioid ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80315-0
更新日期:1987-11-02 00:00:00
abstract::Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the poly-protein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immuno-precipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80069-7
更新日期:1985-11-25 00:00:00