Abstract:
:In order to study the function of the bovine MAP4 microtubule-binding domain (the assembly-promoting (AP) sequence region), a fragment corresponding to the AP sequence region was prepared using an Escherichia coli expression system. When the fragment was mixed with purified tubulin at 37 degrees C, the fragment caused a time- and dose-dependent turbidity increase, and the fragment bound to tubulin. However, the products were cold-stable, and amorphous aggregates were observed by electron microscopy. Using axonemes as the seeds for microtubule assembly, the microtubule-elongating activity of the fragment was examined. A dose-dependent turbidity increase of the sample was observed, and electron microscopic observation revealed that microtubules were dose-dependently elongated from the axonemes. Consequently, the AP sequence region does not nucleate microtubules, but elongates them.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Katsuki M,Tokuraku K,Nakagawa H,Murofushi H,Kotani Sdoi
10.1016/s0014-5793(97)01342-2subject
Has Abstractpub_date
1997-11-24 00:00:00pages
35-8issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01342-2journal_volume
418pub_type
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