Abstract:
:It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric transporters were constructed and their substrate specificities determined. Surprisingly, it is sufficient to replace the first part of the external loop 1 of the HUP1 symporter by the corresponding portion of HUP2 to improve transport and also to decrease the Km value for D-galactose. Additional data indicating the importance of the first loop for substrate recognition and binding are discussed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Will A,Tanner Wdoi
10.1016/0014-5793(96)00097-xsubject
Has Abstractpub_date
1996-02-26 00:00:00pages
127-30issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(96)00097-Xjournal_volume
381pub_type
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