Abstract:
:Tert-butyl hydroperoxide (BHP), hydrogen peroxide and diamide caused a rapid and simultaneous release of glutathione disulfide (GSSG) and K+ in the isolated perfused rat liver. Both BHP-induced effluxes were suppressed by prior depletion of hepatic glutathione, but not by co-infusion of desferrioxamine which prevented lipid peroxidation and cell death. High K+ media decreased the GSSG efflux even though hepatic GSSG levels remained high. The GSSG and K+ effluxes were repeatable if cellular K+ recovered after a short BHP exposure. Ouabain inhibited the K+ re-uptake and decreased the response to repeated BHP challenge. Thus, sinusoidal efflux of GSSG under oxidative stress may be driven by a K+ gradient.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Masuda Y,Ozaki M,Aoki Sdoi
10.1016/0014-5793(93)81692-ssubject
Has Abstractpub_date
1993-11-08 00:00:00pages
109-13issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(93)81692-Sjournal_volume
334pub_type
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